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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-propylvaleronitrile
EC Number:
236-338-2
EC Name:
2-propylvaleronitrile
Cas Number:
13310-75-3
Molecular formula:
C8H15N
IUPAC Name:
2-propylpentanenitrile

Sampling and analysis

Analytical monitoring:
yes
Remarks:
GC-FID

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Strain No. 61.81 SAG

Study design

Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
Microscopic examination of the shape of the algal cells after 72 hours.
Determination of cell density by spectrophotometrical measurement after 24h, 48h, 72h.

Test conditions

Hardness:
0,24 mmol/L (=24 mg/L) as CaCO3
Test temperature:
22.4 to 23.5°C (water temperature)
pH:
pH values at test start 8.2 to 8.3, at the end of the test 8.6 to 9.9
Dissolved oxygen:
Not specified
Nominal and measured concentrations:
Nominal: 0.32, 1.0, 3.2, 10, 32, 100 mg test item/L
Measured: 1.20, 1.20, 1.20, 6.90, 18.2, 51.2 mg test item/L
Details on test conditions:
Continuous illumination (mean light intensity: 5873 lux)
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
<= 27.5 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
<= 6.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
<= 18.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
<= 14.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
<= 6.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
<= 18.2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the experimental results, the 72-h EyC50 (yield) was calculated to be 14.7 mg test item/L and the 72-h ErC50 (growth rate) was calculated to be 27.5 mg test item/L.
The 72-h NOEyC (yield) was determined to be 6.90 mg test item/L and the associated 72-h LOEyC (yield) was 18.2 mg test item/L.
The 72-h NOErC (growth rate) was determined to be 6.90 mg test item/L and the associated 72-h LOErC (growth rate) was 18.2 mg test item/L.