Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 414-310-2 | CAS number: 191358-81-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-12-18 to 2008-05-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline Study (OECD 202) including analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF Test Guidelines, 2-7-2-1, Daphnia immobilization, 2001
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: limit test concentration and control
- Sampling method: For the determination of the actual test item concentrations, duplicate samples were taken from each treatment before the start of the test and at the end of the test after 48 hours.
For the 48-hour stability samples additional flasks with adequate volumes of the freshly prepared test medium and of the control were incubated during the test period under the same conditions as in the actual test (but without daphnids). Sampling from the test beakers with daphnids was not possible because the test medium volume per beaker (50 mL) was too small for the analytical requirements (400 mL per sample).
- Sample storage conditions before analysis: All samples were stored deep-frozen (at about -20 °C) immediately after sampling. Based on pre-experiments for investigation of the storage stability (without GLP), the test item was found to be sufficiently stable in the test water under these storage conditions.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
A limit test was performed in accordance with the test guidelines to demonstrate that the test item has no toxic effect on the daphnids up to the loading rate of 100 mg/L. Thus, a single loading rate of 100 mg/L was tested.
- Controls:
Additionally, a control was tested in parallel (test water without addition of the test item). The limit test was based on the results of a range-finding test and on results of a pre-experiment to determine the solubility of the test item in the test water.
- Test medium:
For the preparation of the test medium, a dispersion of the test item with the loading rate of 100 mg/L was prepared. The dispersion was stirred for 3 hours to dissolve a maximum amount of the different compounds of the test item in the dispersion. Then, the dispersion was filtered through a membrane filter (0.45 μm pore size) and the undiluted filtrate was tested as WAF.
- Standard Solutions for Sample Quantification:
The test item (20.14 mg) was dissolved in dimethylacetamid, made up to the mark in a 100 mL volumetric flask and sonicated for 10 minutes to prepare a stock solution of 201 mg/L. Defined volumes of this stock solution were diluted with dimethylacetamid to obtain standard solutions of the test item in the range of 0.0504 to 20.1 mg/L.
These solutions were used to calibrate the HPLC-system.
- Preparation of Spiked Test Water Samples:
The test item (22.24 mg) was dissolved in dimethylacetamid, made up to the mark in a 100 mL volumetric flask and sonicated for 10 minutes to prepare a stock solution of 222 mg/L. 1 mL of this stock solution was made up to 100 mL with dimethylacetamid to obtain a solution with a concentration of 2.22 mg/L of the test item. 0.5 mL of this solution was diluted with test water to obtain spiked samples with a concentration of 0.00278 mg/L. This solution was freshly prepared in duplicate and subjected to the same treatment as a sample without any storage.
In addition, test water without the test item was analyzed (analytical blank).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- The study was performed with young daphnids of the species Daphnia magna Straus.
- Source: A clone of this species (defined by the supplier as clone 5) was originally supplied by the University of Sheffield / UK in 1992. Since that time, the clone has been bred in the laboratories of RCC in reconstituted water of the quality identical to the water quality used in the tests (in respect to pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests.
- Age at study initiation: At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20 °C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- 8.0 - 8.4 mg/L
- Salinity:
- Alkalinity: 0.8 mmol/L;
- Nominal and measured concentrations:
- Loading rate: 100 mg/L;
Measured concnetration: 0.000161 mg/L; - Details on test conditions:
- TEST SYSTEM
A static test without test medium renewal was performed.
- Test vessel: The test was performed in 100-mL glass beakers filled with 50 mL of test medium.
- Type: The test vessels were covered with glass plates to reduce the loss of water by evaporation and to avoid the entry of dust into the solutions.
The test was performed in a temperature-controlled room with continuous monitoring of the room temperature.
TEST MEDIUM / WATER PARAMETERS
- Photoperiod: A 16-hour light to 8-hour dark cycle with a 30 minute transition period was used.
- Light intensity: Light intensity during the light period was between approximately 490 and 650 Lux.
- The daphnids were not fed during the test.
- No. of organisms per vessel: For each treatment, 20 daphnids were used divided into four replicates of five daphnids each.
- The volume of test solution provided for each daphnia was 10 mL.
- The daphnids were randomly distributed to the test vessels at initiation of the test. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: EC50 was estimated; no acute toxic effects within the range of solubility.
- Details on results:
- A limit test was performed in accordance with the test guidelines to demonstrate that water accommodated fractions (WAFs) of the test item have no toxic effect on Daphnia magna up to the loading rate of 100 mg/L. Thus, the only concentration tested was a WAF with the loading rate of 100 mg/L in parallel to a control.
In the control and at the loading rate of 100 mg/L, no immobilized daphnids were determined during the test period of 48 hours.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- With high probability not acutely harmful to aquatic invertebrates. No acute toxic effects within the range of solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Deși ECHA oferă o varietate bogată de materiale online în limba dumneavoastră, o parte din conţinutul acestei pagini este disponibil numai în limba engleză. Pentru mai multe informaţii referitoare la practica multilingvă a ECHA.
Bun venit pe site-ul ECHA. Acest site nu este deplin acceptat în Internet Explorer 7 (și versiuni anterioare). Vă rugăm să vă actualizați versiunea de Internet Explorer.
Acest site folosește module cookie pentru a garanta că aveți parte de cea mai bună experiență pe paginile noastre.
Aflați mai multe despre cum folosim modulele cookie.