Trisodium 1-amino-4-[[3-[[4-chloro-6-[(sulphonatophenyl)amino]-1,3,5-triazin-2-yl]amino]-2,4,6-trimethyl-5-sulphonatophenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

Method B-21

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 41001/F
Purity: >90%

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

activated sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

pH:

7.4 - 7.6

Dissolved oxygen:

4.7 - 5.1

Nominal and measured concentrations:

1, 3.2, 10, 32, 100 and 320 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Results with reference substance (positive control):

3h IC50 for 3,5 - Dichlorophenol = 11 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The IC50 of FAT 41001/F was found to be greater than 320 mg/L.

Executive summary:

A study was performed with FAT 41001/F to determine the toxicity to microorganisms. This test was carried out in accordance to OECD test guideline 209 and CIBA internal method B-21.

The concentration of test substance used: 1, 3.2, 10, 32, 100, 320 mg/L. 3,5 - Dichlorophenol was used as reference substance.

Based on the findings of the study, the 3h IC50 for FAT 21030/A was found to be greater than 320 mg/L. The 3h IC50 of reference substance was determined to be 11 mg/L.

Description of key information

3-hour IC50 of FAT 41001 to activated sludge micro-organisms was determined to be >320 mg/L. The most recent studies to evaluate the inhibitory effect of the test item FAT 41001 on the respiration rate of aerobic wastewater micro-organisms of activated sludge in a 3-hour respiration inhibition test according to the OECD Guideline for Testing of Chemicals, No. 209 were performed in 1992. The 3-hour IC50 of FAT 41001 to activated sludge micro-organisms was determined to be >320 mg/L. Several other studies performed between 1982 to 1984 reported 24-hour IC50 to be >300 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

320 mg/L