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EC number: 262-134-8 | CAS number: 60270-33-9
Endpoint summary
Administrative data
Description of key information
Additional information
BIODEGRADATION IN WATER: SCREENING TEST
The biodegradation of DIMAPDO lactate was investigated over a 28-day period in a CO2 Evolution Test according to EC method C.4-C (92/69/EEC) and OECD guideline 301 B (1992). The test medium was inoculated with microorganisms from the aeration stage of a sewage treatment plant, which treats predominantly domestic sewage.
The test substance was dispersed in reverse osmosis water with the help of ultrasonication to give a 200 mg/L stock dispersion. An aliquot of this stock dispersion was dispersed in inoculated culture medium to give a final concentration of 13.1 mg/L (9.17 mg C/L). The test solutions were aerated by the passage of CO2 free air at a controlled rate in closed flasks at 21°C for 28 days. The rate of degradation was monitored by measuring the carbon dioxide produced over the 28-d period. The amount of carbon dioxide produced by the microbial population during biodegradation of the test item at a concentration of 13.1 mg/L, corrected for that derived from the blank inoculum run in parallel, was expressed as a percentage of the nominal DOC loading initially present. In order to check the procedure, sodium benzoate was used as a degradable reference item at a concentration of 10 mg C/L, along with a toxicity control at 13.1 mg/L DIMAPDO lactate, and 10 mg C/L sodium benzoate.
The degradation of DIMAPDO lactate in the static test was found to be 120% after 28 days. Biodegradation within the 10-day-window was found to be 87% at day 10.
Based on the worst-case assumption that during biodegradation first only the lactic acid is degraded and only then the degradation of DIMAPDO starts the degradation of DIMAPDO within the 10-day-window can be calculated based on the number of carbon atoms as 85.6%. Therefore the degradation of DIMAPDO satisfied the 10-day window validation criterion even under this worst-case assumption.
The degradation of the reference substance sodium benzoate had reached 100% within the first 14 days. The difference of extremes of replicate values of the removal of the test item at the end of the test is 0.2%. Therefore, the test can be considered as valid.
No inhibitory effects of the test item were observed (more than 25% degradation occurred within 14 days) in the toxicity control.
DIMAPDO fulfilled the 10-day window criterion and the test substance can be regarded as readily biodegradable.
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