Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 287-828-8 | CAS number: 85586-25-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 28 Oct - 01 Nov 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids, C12-18, Me esters
- EC Number:
- 273-095-1
- EC Name:
- Fatty acids, C12-18, Me esters
- Cas Number:
- 68937-84-8
- IUPAC Name:
- 68937-84-8
- Details on test material:
- - Name of test material (as cited in study report): C12-18 fatty acid methyl ester
- Analytical purity: 100% AS
- Expiration date of the lot/batch: until 12 Oct 1992
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighing of test substance into test solution, careful stirring
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: Company "Westaquarium"
ACCLIMATION
- Acclimation period: 8 weeks
- Acclimation conditions (same as test or not): same as test, except for feeding
- Type and amount of food: Altromin 14513, specification 1410, identical with 1413, but pelleted instead of milled
- Health during acclimation (any mortality observed): <0.1% mortality in the week prior to testing
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- not relevant
Test conditions
- Test temperature:
- 22 - 23 °C
- pH:
- Test begin: 8.2-8.3
Test end: 7.8-8.0 - Dissolved oxygen:
- Test begin: 93-97%
Test end: 65-77% (lowest oxygen saturation with the highest TS concentration) - Nominal and measured concentrations:
- Nominal: Control, 100, 300 and 1000 mg/L
Measured (as DOC): for 100 mg/L 1.2 to 6.1 mg/L were measured at 0 and 24 hours and with new solution at 24 and 48 hours; for 300 mg/L, 1.7 to 6.3 mg/L were measured at 0 and 24 hours and with a new solution at 24 and 48 hours; in all cases a random distribution from within the range. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 550 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: Only the soluble fraction of the TS could be found with the analytical testing, however, the remainder of the TS remained in disperse or seperated form in the test solution (no volatility of substance and according to the oxygen values also no biodegradation). The testing laboratory stated no problems with the test substance. however, considering the low water solubility of the substance, the observed effects are expected to be caused rather by physical interference with undissolved particles than due to toxicity
- Reported statistics and error estimates:
- Determination of LC50 according to Stephan (Square root of LC0 times LC100)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mortality in %:
Concentration
Incubation time (h)
in mg/L
0
6
24
48
72
96
0
0
0
0
0
0
0
100
0
0
0
0
0
0
300
0
0
0
0
0
0
1000
0
0
0
30
90
100
Applicant's summary and conclusion
- Executive summary:
No effects up to the limit of water solubility (WS < 1 mg/L)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.