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EC number: 246-870-7 | CAS number: 25339-53-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Decene
- EC Number:
- 246-870-7
- EC Name:
- Decene
- Cas Number:
- 25339-53-1
- Molecular formula:
- C10H20
- IUPAC Name:
- dec-1-ene
- Details on test material:
- - Name of test material (as cited in study report): n-Decene (Commercial name: n-OLEFINA C10)
- Substance type: pure active substance
- Physical state: liquid
- Stability under test conditions: no data
- Storage condition of test material: no data
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: from the aeration basin of the municipal water treatment plant of the city of Novara, Italy
- Laboratory culture: no
- Storage conditions: aerated at room temperature
- Storage length: 24 h
- Preparation of inoculum for exposure: no data
- Pretreatment: no data
- Concentration of sludge: 30 mg/L suspended solids
- Initial cell/biomass concentration: no data
- Water filtered: no data - Duration of test (contact time):
- 30 d
Initial test substance concentration
- Initial conc.:
- 9.3 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to Standard Operating Procedures of the Test Institute, no details mentioned
- Test temperature: 22 ± 0.2 °C
- pH: no data
- Aeration of dilution water: no data
- Suspended solids concentration: 30 mg/L
- Continuous darkness: no data
TEST SYSTEM
- Culturing apparatus: closed flasks with a test volume of 500 mL, no further details mentioned
- Number of culture flasks/concentration: 2
- Measuring equipment: volume reduction and calculated oxygen uptake were measured daily, no further details mentioned
- Details of trap for CO2: evolved CO2 was absorbed in soda lime
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no
STATISTICAL METHODS: no statistics performed
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 15.5 mg/L
Results and discussion
- Preliminary study:
- not performed
- Test performance:
- Biodegradability was assessed by means of manometric respirometry. A measured volume (500 mL) of inoculated medium containing a known amount of test substance (18.6 mg) was stirred in a closed flask. The consumption of oxygen was determined from the reduction in volume of the air contained in the apparatus. The test compound was directly added to the test flasks by means of a microsyringe due to insolubility in water. For each day, volume reduction and calculated oxygen uptake for the test compound and for the blank, in duplicate, were reported together with the corrected values. The same set of values for the reference control was also reported.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 80.9
- Sampling time:
- 28 d
- Details on results:
- Lag phase: 3 days
Degradation phase: 14 days
10-day window: days 3 to day 13
Degradation at the end of the 10-day window: 64.9 % (mean)
Degradation at plateau: ¿ 81 % (mean)
BOD5 / COD results
- Results with reference substance:
- The reference compound reached the pass-level of 60% at day 5.
Any other information on results incl. tables
Table: Biodegradation kinetics
Time [days] | % biodegradation | |||
test 1 | test 2 | mean | reference substance | |
1 | 0 | 0 | 0 | 14.8 |
2 | 4.5 | 6.5 | 5.5 | 39.7 |
5 | 28.5 | 27.5 | 28.0 | 68.1 |
6 | 35.5 | 33.5 | 34.5 | 74.7 |
7 | 39.6 | 39.6 | 39.6 | 74.7 |
8 | 45.6 | 44.6 | 45.1 | 78.0 |
9 | 50.9 | 50.9 | 50.9 | 80.8 |
12 | 63.8 | 63.8 | 63.8 | 80.7 |
13 | 64.0 | 65.9 | 64.9 | 83.3 |
14 | 67.1 | 70.1 | 68.6 | 86.0 |
15 | 67.1 | 70.1 | 68.6 | 86.0 |
16 | 70.8 | 72.8 | 71.8 | 86.9 |
19 | 74.3 | 75.3 | 74.8 | 84.9 |
21 | 76.1 | 76.1 | 76.1 | 84.9 |
22 | 78.8 | 78.8 | 78.8 | 85.7 |
23 | 79.3 | 79.3 | 79.3 | 84.9 |
26 | 81.0 | 83.0 | 82.0 | 87.1 |
28 | 80.9 | 80.9 | 80.9 | 88.0 |
30 | 78.9 | 80.9 | 79.9 | 86.9 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance n-OLEFINA C10 was readily biodegradable, measured by manometric respirometry.
The reported test was considered valid meeting the following conditions:
Oxygen uptake of inoculum blank was less than 60 mg/L at day 28, the difference between replicate values was less than 20% at day 28, percentage of degradation of reference control reached 60% pass-level before day 14. - Executive summary:
The biodegradation of the test substance n-Decene measured by manometric respirometry according to OECD 301F was 80.9 % after 28 days of incubation.
The full set of relevant degradation parameters, as derived from the graph of percentage degradation , was as follows:
Lag phase: 3 days
Degradation phase: 14 days
10-day window: days 3 to day 13
Degradation at the end of the 10-day window: 64.9 % (mean)
Degradation at plateau: ¿ 81 % (mean)
Therefore the test substance was considered to be readily biodegradable.
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