Tin sulfide (Sn2S3)

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2014-03-26 - 2014-11-07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no GLP, conducted on a structural analogous read-across substance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)

Version / remarks:

, 14 June 1996

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1730 (Fish Bioconcentration Test)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EU Method C.13 (Bioconcentration: Flow-through fish test)

Version / remarks:

, (2008)

Deviations:

no

GLP compliance:

no

Remarks:

Test performed for another registration

Test material

Test material information

Constituent 1

Reference substance name:

Tin sulphide

EC Number:

215-248-7

EC Name:

Tin sulphide

Cas Number:

1314-95-0

Molecular formula:

SSn

IUPAC Name:

Tin(II) sulfide

Constituent 2

Reference substance name:

Tin sulfide

IUPAC Name:

Tin sulfide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Radiolabelling:

no

Test solutions

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

A supersturated solution of WAFS was used. Therefore the test item was added and stirred for 24 hours on a magnetic stirplate at 23- 25°C. After stirring was stopped test substance settled down for one hour. The aqueous part was removed and used as standard solution.
Under exposure conditions the solution was changed in interval of 96 hours (qualitiy control).Fresh test solution was prepared 24 hours in advance.

Test organisms

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

TEST ORGANISM

- Source: Fish supplier of Institute of Hydrobiology, Chinese Academy of Sciences
- Length at study initiation (lenght definition, mean, range and SD): 2.6 +- 0.5 cm
- Weight at study initiation (mean and range, SD): 0.20 +- 0.1 g
- Food type: same as during acclimation
- Amount/feeding frequency: 1-2% of body weight per day
-

ACCLIMATION
- Acclimation period: 15 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: proprietary fish food (Protein < 36%, Fat > 2%, Fibre < 3%, Ash < 12%, Moisture < 10.0%
- Feeding frequency: dayly
- Health during acclimation (any mortality observed): no mortality, free from diseases and abnormalities

Study design

Route of exposure:

aqueous

Test type:

semi-static

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Test conditions

Hardness:

119 - 122 mg/L CaCO3

Test temperature:

22.2 - 22.5°C

pH:

7.76 - 7.84

Dissolved oxygen:

76 - 79%

Reference substance (positive control):

no

Details on estimation of bioconcentration:

Sampling and sample treatment:
At each sampling time 7 fish were removed, were killed instantly and were weigh. For calculation of test item concentration in fish, the named 7 samples were whetted with anhydrous sodium sulfat and were digested by adding 50 ml mixture of 65% nitric acid and 70% perchloric acid. In addition samples were dried and dissolved. Final solution was injected to ICP-MS after filtering with 0.22 µm millipore membran.

Water samples were divided in to two (unfiltered sample and a filtered sample through 0.22 µm millipore membran). Water samples were analysed by ICP-MS.

Results and discussion

Bioaccumulation factor

Type:

BCF

Value:

>= 24 - <= 558 dimensionless

Time of plateau:

7 d

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0.01 mg/L

Reported statistics:

Test Validitiy:
- temperature was maintained at 23 +-2 °C
- dissolved oxygen does not fall below 60% saturation
- less than 10% mortality or adverse effects in control/ treated group

Data Processing:
7 BCF values were calculated. After 0, 3, 7, 10, 14, 21, 28 days a sample of seven fish and water samples were used for calculation. Stady state was
calculated by plotting the concentration of test item in fish against the time. Stady state was reached, if the uptake curve become approximately asymptotic to time axis (changing extent did not exceed +- 20%).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

During the test (28 d) the range of the BCFss value of test substance is 24 - 558, when exposed to the supersaturated solution of the test item tin sulfide.
Accordingly, also ditin trisulfide is not expected to be bioaccumulative.

Executive summary:

The bioconcentration factor of tin sulfide in fish (Gobiocypris rarus) was determined with reference to OECD Guideline 305B "Bioconcentration: Semistatic Fish-Test". Fish were exposed for 28 days. After 0, 3, 7, 10, 14, 21, 28 days of exposure (0.01 mg/L test item in solution) a sample of seven fish and water samples were used for calculation of BCF. Stady state was calculated by plotting the concentration of test item in fish against the time, if the uptake curve become approximately asymptotic to time axis (changing extent did not exceed +- 20%). The results show that BCF reach the steady state at seventh day.Therefore, the steady-state period BCFss for fish was calculated from day 7 to day 28, and the mean is 558 and 24.0, respectively, according to the solubility of 0.6 µg/L of test item tin sulfide and a messured exposure concentration of 14 µg/L.