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EC number: 253-452-8 | CAS number: 37294-49-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 18, 2013 - July 17, 2014
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was performed in accordance with OECD guideline 111. Non-GLP study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Version / remarks:
- (2004)
- GLP compliance:
- not specified
- Remarks:
- no information
Test material
- Reference substance name:
- Disodium C-isodecyl sulphonatosuccinate
- EC Number:
- 253-452-8
- EC Name:
- Disodium C-isodecyl sulphonatosuccinate
- Cas Number:
- 37294-49-8
- Molecular formula:
- C14H26O7S.2Na
- IUPAC Name:
- disodium 4-[(2-methylnonyl)oxy]-4-oxo-3-sulfonatobutanoate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Name of test material (as cited in study report): Disodium Isodecyl Sulfosuccinate
Description: white powder
Storage conditions of test material: at room temperature in well-sealed container
The substance was tested as received.
Constituent 1
- Radiolabelling:
- no
Study design
- Analytical monitoring:
- yes
- Details on sampling:
- A sample (5 mL) was taken and diluted to 10.0 mL with methanol in a volumetric flask. The resulting solution was shaken well before HPLC analysis. Blank buffer solutions and standard solutions were treated in the same way.
- Buffers:
- pH 4 buffer solution - potassium acid phthalate
pH 7 buffer solution - potassium phosphate monobasic and sodium hydroxide, chlorine titrimeter reagent
pH 9 buffer solution - boric acid, potassium chloride, and sodium hydroxide
The buffers were filtered through a 0.1 µm syringe filter and degassed by ultrasonication. - Details on test conditions:
- -Tier 1 preliminary test at pH 4, 7 and 9 and 50°C
-Tier 2 main study at pH 9 and 65°C, 50°C and 20°C
-The test solutions were incubated in a liquid bath or incubator in the dark at the specific test temperature (± 0.5°C).
-Each sample was injected in triplicate into the HPLC system.
-All equipment and glassware was sterilized.
Details Tier 1 preliminary test
-Test substance solutions were prepared (in duplicate) in the buffer solutions pH 4, 7 and 9 at concentrations ranging from 2.9 and 3.5 g/L.
-Samples were taken from the test substance solutions after 120 h incubation at 50°C.
-Standards were prepared in buffer solution pH 4, 7 and 9 at a concentration of 3 g/L.
-The standards were treated in the same way as the test samples (see Details on sampling).
-The mean (n=3) peak area of each standard or sample was multiplied by the correction factor '3.0/concentration prepared' (see table below) in order to be able to compare the peak areas of the samples with the standards and to calculate the '% of mean initial concentration'.
Details Tier 2 main study
-Test substance solutions were prepared at nominal 3 g/L (two solutions per test temperature).
-Samples were taken from the test substance solution after preparation and after 15, 24, 46, 66, 72, 139 and 216 h of incubation at 65°C.
-Samples were taken from the test substance solution after preparation and after 15, 24, 46, 72, 139, 216 h and 689.5 h of incubation at 50°C.
-Samples were taken from the test substance solution after preparation and after 48, 196, 263, 426.5 h, 719 h and 744 h of incubation at 20°C.
-On each day of analysis standards were prepared in buffer solution pH 9 at a concentration of 3 g/L.
-The standards were treated in the same way as the test samples (see Details on sampling).
-The mean (n=3) peak area of each standard or sample was multiplied by the correction factor '3.0/concentration prepared' (see table below).
-The % peak area relative to the peak area of the fresh standard was calculated (=% of mean initial concentration) (see table below).
-Ct=Concentration at time t = 3.0 x '% of mean of initial concentration' (see table below).
Duration of testopen allclose all
- Duration:
- 216 h
- pH:
- 9
- Temp.:
- 65 °C
- Initial conc. measured:
- 2.99 - 3.01 g/L
- Duration:
- 689.5 h
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 2.99 - 3.01 g/L
- Duration:
- 744 h
- pH:
- 9
- Temp.:
- 20 °C
- Initial conc. measured:
- 2.99 - 3.02 g/L
- Number of replicates:
- two (A and B)
- Positive controls:
- no
- Negative controls:
- no
Results and discussion
- Preliminary study:
- At pH 4 and pH 7, hydrolysis < 10% was observed after 5 days. This corresponds with a half-life time at 25°C of > 1 year (half-life time at 25°C is > 1 year). According to the guideline, no further tests were required at pH 4 and pH 7.
A degree of > 10% of hydrolysis was observed after 5 days at pH 9 and 50°C. In accordance with the guideline, a Tier 2 test was performed at pH 9. - Transformation products:
- not measured
- Details on hydrolysis and appearance of transformation product(s):
- In general carboxylic esters hydrolyze to the carboxylic acid and an alcohol. Proposed hydrolysis product: Sulphonatosuccinate anion; C4H5O7S.
Dissipation DT50 of parent compoundopen allclose all
- pH:
- 9
- Temp.:
- 65 °C
- Hydrolysis rate constant:
- 0.013 h-1
- DT50:
- 53.8 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: y= -0.0129x + 1.0064; r2=0.9905
- pH:
- 9
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.003 h-1
- DT50:
- 226.5 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: y= -0.00306+1.0692;r2=0.9984
- pH:
- 9
- Temp.:
- 20 °C
- Hydrolysis rate constant:
- 0 h-1
- DT50:
- 5 338 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: Y= -0.00013x + 1.1322; r2=0.5396
- pH:
- 9
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0 h-1
- DT50:
- 2 874.6 h
- Type:
- other: from the Arrhenius plot
- Remarks on result:
- other: t1/2= 120 days
- Details on results:
- No significant peaks were observed at the approximate retention time of the test substance on analysis of any matrix blank solutions.
Any other information on results incl. tables
Table: Results of the preliminary (Tier 1) test at pH 4, 7 and 9 at 50°C
pH |
Solution |
Concentration prepared (g/L) |
Mean (n=3) peak area |
Corrected peak area |
% of Mean Initial Concentration |
4 |
Standard A |
3.4 |
786.6 |
684.0 |
100 |
Sample 1 (120 h) |
3.0 |
726.3 |
719.1 |
105.1 |
|
Sample 2 (120 h) |
2.9 |
692.9 |
729.4 |
106.6 |
|
7 |
Standard B |
3.5 |
795.9 |
684.2 |
100 |
Sample 3 (120 h) |
3.5 |
796.3 |
692.5 |
101.2 |
|
Sample 4 (120 h) |
3.3 |
796.7 |
715.6 |
104.6 |
|
9 |
Standard C |
3.0 |
670.2 |
667.9 |
100 |
Sample 5 (120 h) |
3.0 |
485.6 |
482.4 |
72.2 |
|
Sample 6 (120 h) |
3.2 |
509.0 |
465.6 |
69.7 |
Table: Tier 2 results of the test at pH 9 and 65°C
Solution |
Mean (n=3) peak area |
Corrected peak area |
% of Mean Initial Concentration |
Initial sample A, 3.01 mg/mL |
1061.7 |
1055.9 |
|
Initial sample B, 2.99 mg/mL |
1085.3 |
1088.4 |
|
Standard 1, 3.02 mg/mL |
1041.6 |
1033.3 |
|
15 hrs, sample A |
807.9 |
803.5 |
77.8 |
15 hrs, sample A |
805.8 |
808.1 |
78.2 |
24 hrs, sample A |
714.1 |
710.2 |
68.7 |
24 hrs, sample B |
716.8 |
718.8 |
69.6 |
Standard 2, 3.02 mg/mL |
1017.9 |
1009.9 |
|
46 hrs, sample A |
504.8 |
502.0 |
49.7 |
46 hrs, sample B |
588.6 |
590.2 |
58.4 |
Standard 3, 3.02 mg/mL |
996.9 |
990.3 |
|
66 hrs, sample A |
364.1 |
362.1 |
36.6 |
66 hrs, sample B |
364.2 |
365.3 |
36.9 |
72 hrs, sample A |
349.3 |
347.4 |
35.1 |
72 hrs, sample B |
339.2 |
340.2 |
34.4 |
Standard 4, 3.04 mg/mL |
1017.8 |
1004.4 |
|
139 hrs, sample A |
137.2 |
136.1 |
13.6 |
139 hrs, sample B |
130.8 |
130.4 |
13.1 |
Standard 5, 3.00 mg/mL |
1006.9 |
1005.6 |
|
216 hrs, sample A |
59.8 |
59.3 |
5.9 |
216 hrs, sample B |
65.8 |
65.5 |
6.6 |
Table: Tier 2 results of the test at pH 9 and 50°C
pH 9 Buffer Solution |
Mean (n=3) peak area |
Corrected peak area |
% of Mean Initial Concentration |
Initial sample A, 3.01 mg/mL |
1061.7 |
1055.9 |
|
Initial sample B, 2.99 mg/mL |
1085.3 |
1088.4 |
|
Standard 1, 3.02 mg/mL |
1041.6 |
1033.3 |
|
15 hrs, sample A |
989.1 |
983.7 |
95.2 |
15 hrs, sample A |
975.2 |
977.9 |
94.6 |
24 hrs, sample A |
929.9 |
924.8 |
89.5 |
24 hrs, sample B |
944.0 |
946.7 |
91.6 |
Standard 2, 3.02 mg/mL |
1017.9 |
1009.9 |
|
46 hrs, sample A |
876.1 |
871.3 |
86.3 |
46 hrs, sample B |
868.1 |
870.5 |
86.2 |
Standard 3, 3.02 mg/mL |
996.9 |
990.3 |
|
72 hrs, sample A |
776.2 |
772.0 |
78.0 |
72 hrs, sample B |
739.7 |
741.8 |
74.9 |
Standard 4, 3.04 mg/mL |
1017.8 |
1004.4 |
|
139 hrs, sample A |
634.3 |
628.9 |
62.6 |
139 hrs, sample B |
636.3 |
634.2 |
63.1 |
Standard 5, 3.00 mg/mL |
1006.9 |
1005.6 |
|
216 hrs, sample A |
505.8 |
503.1 |
50.0 |
216 hrs, sample B |
471.6 |
472.9 |
47.0 |
Standard 7, 3.03 mg/mL |
931.6 |
921.8 |
|
689.5 hrs, sample A |
113.8 |
113.2 |
12.3 |
689.5 hrs, sample B |
106.1 |
106.4 |
11.5 |
Table: Tier 2 results of the test at pH 9 and 20°C
pH 9 Buffer Solution |
Mean (n=3) peak area |
Corrected Peak area |
% of Mean Initial Concentration |
Initial sample C, 2.99 mg/mL |
959.2 |
960.6 |
|
Initial sample D 3.02 mg/mL |
993.2 |
993.7 |
|
Standard 8, 3.05 mg/mL |
948.2 |
932.0 |
|
48 hrs, sample C |
923.8 |
925.2 |
99.3 |
48 hrs, sample D |
980.5 |
981.0 |
105.3 |
Standard 9, 3.07 mg/mL |
893.4 |
873.6 |
|
196 hrs, sample C |
877.4 |
878.7 |
100.6 |
196 hrs, sample D |
930.1 |
930.6 |
106.5 |
Standard 10, 3.04 mg/mL |
965.2 |
952.5 |
|
263 hrs, sample C |
909.5 |
910.8 |
95.6 |
263 hrs, sample D |
955.6 |
956.1 |
100.4 |
Standard 11, 3.06 mg/mL |
936.6 |
918.2 |
|
263 hrs, sample C |
873.4 |
874.7 |
95.3 |
263 hrs, sample D |
911.5 |
912.0 |
99.3 |
Standard 12, 3.07 mg/mL |
976.4 |
954.8 |
|
426.5 hrs, sample C |
902.8 |
904.1 |
94.7 |
426.5 hrs, sample D |
938.9 |
939.4 |
98.4 |
Standard 13, 2.98 mg/mL |
948.1 |
953.2 |
|
719 hrs, sample C |
881.3 |
882.6 |
92.6 |
719 hrs, sample D |
936.7 |
937.2 |
98.3 |
Standard 14, 3.03 mg/mL |
1002.1 |
991.5 |
|
744 hrs, sample C |
895.7 |
897.0 |
90.5 |
744 hrs, sample D |
944.6 |
945.1 |
95.3 |
Table: Tier 2 results at pH 9 and 65°C
t [h] |
lnCt (Ct in g/L) |
15 |
0.85 |
15 |
0.85 |
24 |
0.72 |
24 |
0.74 |
46 |
0.40 |
46 |
0.56 |
66 |
0.09 |
66 |
0.10 |
72 |
0.05 |
72 |
0.03 |
139 |
-0.90 |
139 |
-0.94 |
216 |
-1.73 |
216 |
-1.63 |
Table: Tier 2 results at pH 9 and 50°C
t [h] |
lnCt (Ct in g/L) |
15 |
1.05 |
15 |
1.04 |
24 |
0.99 |
24 |
1.01 |
46 |
0.95 |
46 |
0.95 |
72 |
0.85 |
72 |
0.81 |
139 |
0.63 |
139 |
0.64 |
216 |
0.41 |
216 |
0.34 |
689.5 |
-1.00 |
689.5 |
-1.06 |
Table: Tier 2 results at pH 9 and 20°C
t [h] |
lnCt (Ctin g/L) |
48 |
1.09 |
48 |
1.15 |
196 |
1.10 |
196 |
1.16 |
263 |
1.05 |
263 |
1.10 |
426.5 |
1.05 |
426.5 |
1.09 |
575 |
1.04 |
575 |
1.08 |
719 |
1.02 |
719 |
1.08 |
744 |
1.00 |
744 |
1.05 |
Applicant's summary and conclusion
- Conclusions:
- The substance is hydrolytically stable at pH 4 and 7 (half-life time at 25°C of > 1 year). Hydrolysis of the test substance at pH 9 shows a pseudo-first order behaviour. Half-life time at 25°C and pH 9 was determined to be 120 days.
- Executive summary:
The rate of hydrolysis of the substance at pH values normally found in the environment (pH 4-9) was determined in a OECD TG 111 study. At pH 4 and 7, a degree of < 10% of hydrolysis was observed after 5 days at 50 °C. This corresponds with a half-life time at 25°C of > 1 year. The substance can be considered hydrolytically stable at pH 4 and 7.
At pH 9 and 50°C, a degree of ca. 30% of hydrolysis was observed after 5 days and therefore a Tier 2 study was performed at pH 9. Hydrolysis rates of the substance at 65, 50 and 20°C were determined from which the hydrolysis rate at 25°C was calculated. The test substance was shown to degrade by a pseudo-first order behaviour. Half-life time at 25°C and pH 9 was determined to be 120 days.
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