6,10-dimethylundeca-5,9-dien-2-one

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed according to OECD and GLP guidelines

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

6,10-dimethylundeca-5,9-dien-2-one

EC Number:

211-711-2

EC Name:

6,10-dimethylundeca-5,9-dien-2-one

Cas Number:

689-67-8

Molecular formula:

C13H22O

IUPAC Name:

6,10-dimethylundeca-5,9-dien-2-one

Method

Target gene:

TA97a: hisD6610, TA 98: hisD3052; TA 100 and TA 1535 hisG46, TA102: hisG428

Species / strain

Species / strain / cell type:

other: TA1535, TA97, TA98, TA100, and TA102

Additional strain / cell type characteristics:

acetyltransferase deficient

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

0, 1.6, 5, 16.6, 50, 166 ug/plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

2-nitrofluorene

sodium azide

mitomycin C

other: 2-aminoanthracene

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA1535, TA97, TA98, TA100, and TA102

Metabolic activation:

with and without

Genotoxicity:

negative

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

-

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Neither Geranylaceton per se, nor any of its metabolites formed under the experimental conditions, induced genetic damage in
the Ames test.

Executive summary:

Geranylaceton was evaluated for mutagenic activity in the Ames test. A standard plate incorporation and a preincubation modification assay in absence and in presence of an exogenous metabolic activation system (S9) were performed. Five

Salmonella typhimurium tester strains (TA1535, TA97, TA98, TA100, and TA102) were

employed. The activity of the S9-mix and the responsiveness of the tester strains were

verified by including appropriate controls into each experiment. Geranylaceton was dissolved in dimethylsulfoxide (DMSO). Toxic effects were observed in a preliminary toxicity experiment, starting at 158 ug/plate (slightly reduced background growth). Therefore concentrations ranging from 1.6 to 166 ug/plate were evaluated in the main experiments. Upon addition to the aqueous

medium, a slight milky suspension was observed at 50 ug/plate (preincubation assay) and 166 ug/plate (both methods). Strain dependent toxicity (reduction in the background growth and/or reduction in the number of revertant colonies) was observed

in the selected concentration range. The toxic effects were more pronounced in absence of an exogenous metabolic activation system and in the experiment using the preincubation method, known to be more sensitive for several class of compounds.

Geranylaceton did not cause a mutagenic effect in any of the five investigated strains.

Thus it can be concluded, that neither Geranylaceton per se, nor any of its metabolites formed under the experimental conditions, induced genetic damage in the Ames test.