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EC number: 248-236-5 | CAS number: 27121-30-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test performed according to relevant guideline and compliant to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- [29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper
- EC Number:
- 248-236-5
- EC Name:
- [29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper
- Cas Number:
- 27121-30-8
- Molecular formula:
- C32H13Cl3CuN8O6S3
- IUPAC Name:
- Copper, [29H,31H-phthalocyanine-C,C,C-trisulfonyl trichloridato(2-)-kN29,kN30,kN31,kN32]-
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling
- Representative sample of total water amount 10L: 20mL
- Appearance of sample : blue solution
- Temperature of water samples during the toxicity testing 22°C+1°C
- 0h, 48h, 96h: one control and two treatment samples respectively
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was weighed into a beaker. Water for dilution was added and the mixture homogenized using an Ultra-Turrax and poured into the chamber under stirring with a glass rod. The chamber contents were then stirred for approximately 30 minutes with a KPG-stirrer and a glass rod. All tested concentrations were present as a blue solution. Slight particulate matter was observed on the bottom of the test chamber.
In order to determine the substance concentration, water samples were taken from the middle of the test chamber by pipetting approx. 20 mL into a screw-neck glass bottle at study start, and after 48 and 96 hours.
The following values were determined during the study:
Nominal concentration: 100 mg/L
Measured concentrations after 0h: 105 mg/L, 98.3 mg/L
Measured concentrations 48h: 101 mg/L, 105 mg/L
Measured concentrations 96h: 103 mg/L, 99.8 mg/L
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Strain: Brachydanio rerio (HAMILTON-BUCHANAN)
- Source: breeding station Corporate Toxicology (Hoechst AG)
- Age at study initiation (mean and range, SD): appr. 7 months
- Length at study initiation (length definition, mean, range and SD): the body length of 7 representative fish from the batch was measured: 2.8 - 3.2 cm (mean 3.0, SD 0.14)
- Food type: Tetra Min, Tetra Werke, Melle
- Frequency: twice daily ad libitum
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test): 22 ± 1 °C, light period 12 h daily, oxygen content > 80 % of the saturation value, population density < 1 g fish/L water (200 L vessel)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- The total hardness of the dilution water was determined weekly and was 2.3 mmol [Ca(2+) + Mg(2+)] / L during the study.
- Test temperature:
- Concentration group: 21.4 - 22.0
Control group: 21.4 - 22.0 - pH:
- Concentration group: 7.8 - 8.2
Control group: 7.9 - 8.2 - Dissolved oxygen:
- [mg/L]
Concentration group: 7.4 - 7.8
Control group: 7.9 - 8.3 - Nominal and measured concentrations:
- Nominal concentration: 100 mg/L (corresponds to 41,5 mg/L registration substance and 58.5 mg/L water)
Measured concentrations after 0h: 105 mg/L, 98.3 mg/L
Measured concentrations 48h: 101 mg/L, 105 mg/L
Measured concentrations 96h: 103 mg/L, 99.8 mg/L
All measured concentrations were in a range of ± 10 % of the theoretical value. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 L glass chambers, 30 cm x 22 cm x 24 cm
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water accordiing to ISO/DIS 7346/1, aeration until oxygen saturation, pH 8.5
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: 700 lux
After the test concentrations had been prepared and water parameters recorded, 7 fish were assigned to each test and control chamber. The fish received no feed for the entire study period. Inspection of the fish took place after 3, 6, 24, 48, 72 and 96 hours and involved recording the lethality and visible changes in appearance and behavior. Dead fish were removed from the chambers. Fish were considered dead when there was a lack of opercular movement and no response to slight mechanical stimulus. The water parameters were measured and recorded before study start and after 0, 24, 48, 72 and 96 hours. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: (corresponds to 41.5 mg/L registration substance and 58.5 mg/L water
- Details on results:
- Lethality:
In this 96-hour acute toxicity study in zebra fish (Brachydanio rerio) lethality occurred neither in the control nor in the 100 mg/L-group .
The LC50 after 96 hours therefore exceeds 100 mg/L
Appearance and behavior:
No changes in appearance and behaviour could be observed, but due to the blue colour of the solution observations were disturbed. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96h acute toxicity test with Brachydanio rerio had been performed as a limit test (poor solubility of test item) according to OECD 203 and compliant to GLP. LC50 (96h) > 100 mg/L nominal (corresponds to 41.5 mg/L registration substance and 58.5 mg/L water).
- Executive summary:
When testing the lethal effect of the test item in zebra fish (Brachydanio rerio) over a total of 96 hours, no lethality occurred at 100 mg/L (nominal) whichcorresponds to 41.5 mg/L registration substance and 58.5 mg/L water. The LC50 after 96 hours therefore exceeds 100 mg/L. The nominal concentration tested was 100 mg/L and a negative control (0 mg/L). No changes in behaviour and appearance could be observed, but the observation was disturbed by the blue colour of the test item solution. The substance concentration was determined in the 100 mg/L group. The measured concentration values were within a range of ± 10 % of the theoretical value.
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