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EC number: 223-912-2 | CAS number: 4118-16-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- 1997
- Deviations:
- yes
- Remarks:
- Difference in treatment schedule (12 week treatment, 3 times a week), No positive control
- GLP compliance:
- not specified
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bisanthraquinone
- EC Number:
- 223-912-2
- EC Name:
- 1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]bisanthraquinone
- Cas Number:
- 4118-16-5
- Molecular formula:
- C37H21N5O4
- IUPAC Name:
- 1,1'-[(6-phenyl-1,3,5-triazine-2,4-diyl)diimino]di(9,10-anthraquinone)
Constituent 1
Test animals
- Species:
- hamster, Chinese
- Strain:
- other: Cricetulus griseus
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: female: 23-30 g, male: 22-34 g
- Diet: NAFAG No. 924
- Water: Tap water ad libitum
- Acclimation period: Air conditioned room
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-24 °C
- Humidity (%): 50-72 %
- Photoperiod: Illuminated for 12 hours
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle used: Polyethylene glycole 400
- Concentration of test material in vehicle: 250, 500, 1000 and 2000 mg/kg in 20 mL/kg PEG 400 - Details on exposure:
- Treatment schedule: The preparation was administered orally to groups of 6 female and 6 male animals each, thrice weekly for twelve weeks. Six hours after the last application the animals were sacrificed by dislocation of the cervical vertebra.
- Duration of treatment / exposure:
- 12 weeks
- Frequency of treatment:
- thrice weekly
- Post exposure period:
- no
Doses / concentrationsopen allclose all
- Dose / conc.:
- 250 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 500 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 1 000 mg/kg bw/day (actual dose received)
- Dose / conc.:
- 2 000 mg/kg bw/day (actual dose received)
- No. of animals per sex per dose:
- 6 males
6 females - Control animals:
- yes, concurrent vehicle
- Positive control(s):
- none
Examinations
- Tissues and cell types examined:
- Bone marrow from the shafts of both femurs.
Cell types examined: Single Jolly bodies, fragments of nuclei in erythrocytes, micronuclei in erythroblasts, micronucleiin leuocpoietic cells, polyploid cells. - Details of tissue and slide preparation:
- PREPARATION OF BONE MARROW:
The animals were sacrificed six hours after the last application. From the bone marrow smears were made.Bone marrow was harvested from the shafts of both femurs. In a siliconized pipette filled with approx. 0.5 µL rat serum the bone marrow was drawn up. In order to receive a homogeneous suspension the content of pipette was aspirated gently about three times. Small drops of the mixture were transferred on the end of a slide, spread out by pulling it behind a polished cover glass and the preparations were airdried. Three hours later, the slides were stained in undiluted May-Griinwald solution for 2 min then in May-Griinwald solution/water 1/1 for 2 min and then in Giemsa's, 40% for 20 min. After being rinsed in methanol, 55% for 5-8 sec and washed off twice in water, they are left immersed in water for approx. 2 min. After rinsing with distilled water and air-drying the slides were cleared in Xylol and mounted in Eukitt.
DETAILS OF SLIDE PREPARATION:
The slides of three female and three male animals per group were examined. 1000 bone marrow cells each were scored per animal and the following anomalies were registered: a) Single Jolly bodies, b) fragments of nuclei in erythrocytes, c) micronuclei in erythroblasts, d) micronucleiin leucopoietic cells, e) polyploid cells. - Statistics:
- The significance of difference was assessed by X^2 test.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Additional information on results:
- Of the twelve animals in the control group one female and one male animal died during the seventh week of the experiment. Of the animals treated with the lowest dose (250 mg/kg) one female and one male animal died during the second week of treatment. In the group treated with 500 mg/kg one male animal of the twelve animals died by the sixth week. Of the twelve animals in the group given 1000 mg/kg of the test substance all survived. In the group treated with the highest dose (2000 mg/kg) two male animals died during the second and the sixth week respectively of the treatment and one male animal died during the seventh week. In all dosage groups the percentage of cells displaying anomalies of nuclei did not differ significantly from the negative control.
Any other information on results incl. tables
|
Number of animals |
Sex of females |
Single Jolly bodies |
Fragments of nuclei |
Micronuclei in |
Micronuclei in |
Polyploid cells |
Total |
Control |
1 |
m |
|
|
|
|
|
0.0 |
(PEG 400) |
2 |
m |
0.1 |
|
|
|
|
0.1 |
|
3 |
m |
0.1 |
|
|
|
|
0.1 |
|
4 |
f |
|
|
|
|
|
0.0 |
|
5 |
f |
0.2 |
|
|
|
|
0.2 |
|
6 |
f |
0.1 |
|
|
|
|
0.1 |
Test item |
1 |
m |
0.1 |
|
0.1 |
|
|
0.2 |
(250 mg/kg) |
2 |
m |
0.1 |
|
|
|
|
0.1 |
|
3 |
m |
|
|
|
|
|
0.0 |
|
4 |
f |
|
|
0.3 |
|
|
0.3 |
|
5 |
f |
0.1 |
|
|
|
|
0.1 |
|
6 |
f |
0.1 |
|
|
|
|
0.1 |
Test item |
1 |
m |
|
|
|
|
|
0.0 |
(500 mg/kg) |
2 |
m |
0.1 |
|
|
|
|
0.1 |
|
3 |
m |
0.2 |
|
|
|
|
0.2 |
|
4 |
f |
|
|
|
|
|
0.0 |
|
5 |
f |
0.1 |
|
|
0.1 |
|
0.2 |
Test item |
6 |
f |
|
|
|
|
|
0.0 |
(1000 mg/kg) |
1 |
m |
|
|
|
|
|
0.0 |
|
2 |
m |
0.1 |
|
|
|
|
0.1 |
|
3 |
m |
0.2 |
|
|
|
|
0.2 |
|
4 |
f |
0.1 |
|
|
|
|
0.1 |
|
5 |
f |
|
|
|
|
|
0.0 |
|
6 |
f |
0.1 |
|
|
|
0.1 |
0.2 |
Test item |
1 |
m |
|
|
|
|
|
0.0 |
(2000 mg/kg) |
2 |
m |
|
|
|
0.1 |
|
0.1 |
|
3 |
m |
0.1 |
|
|
|
|
0.1 |
|
4 |
f |
|
|
|
0.1 |
|
0.1 |
|
5 |
f |
0.4 |
|
|
|
|
0.4 |
|
6 |
f |
0.1 |
|
|
|
|
0.1 |
Applicant's summary and conclusion
- Conclusions:
- The incidence of bone marrow cells with anomalies of nuclei corresponds to the frequency observed in the control group. It is concluded that under the conditions of this experiment, no evidence of mutagenic effects was obtained in Chinese hamsters treated with the test item.
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