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EC number: 269-085-1 | CAS number: 68187-30-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The temperature for the main test was slightly out of the demanded range (17.8 to 21.6 °C instead of 18 to 22 °C). Taking a mean of 19.7 °C the values are not varying more than ± 2 °C. Since no significant effects on reproductive output occurred during the test, this deviation is considered to be uncritical and not invalidating the test. The deviation was signed and assessed by the study director on March 03, 2016.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all treatments
- Sampling method: Samples for analytical determinations were taken from the new and the old solutions at each medium renewal. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution containing nominal 100 mg/L in dilution water was prepared. The solutions of the amount needed were prepared by dilution of this stock solution with dilution water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
-Strain: Berlin
- Source: in-house culture originally obtained from Umweltbundesamt , Berlin
- Age at study initiation (mean and range, SD): < 24h
- Feeding during test: Yes
- Food type: green algae (Desmodesmus subspicatus), 0.15 - 0.2 mg OC/animal/day (amount of carbon was based on photometric measurement) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 267 - 445 mg/L as CaC03
- Test temperature:
- 17.8 - 21.6 °C; Taking a mean of 19.7 °C the values were not varying more than ± 2 °C.
- pH:
- 7.7 - 8.5
- Dissolved oxygen:
- 8.4 - 9.6 mg/L
- Nominal and measured concentrations:
- Nominal: 1.1, 2.2., 4.6, 10 and 22 mg product/L
Nominal: 0.275, 0.55, 1.15, 2,5 and 5.5 mg AS/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mg glass beakers with 80 mL test solution
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): three times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (Elendt M4) is used as media for culture and test purposes
- TOC content: < 2 mg/L
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen content and hardness were measured in the new and the old test solutions (control and highest test concentration) once a week.
OTHER TEST CONDITIONS
- Photoperiod: 16:8 light/dark
- Light intensity: 1000 lux
EFFECT PARAMETERS MEASURED: Mortality, reproduction and other sublethal effects were measured daily. - Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 5.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals: 20%
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is not harmful to aquatic invertebrates at chronic exposure.
Reference
The content of the test item in the test solutions was estimated by DOC measurement. Only in the highest concentrated treatment 22 mg/L, the measured DOC concentrations in the fresh prepared solutions showed the presence of dissolved test item. The measured DOC concentration in the fresh prepared solution of this treatment ranged from 3.6 – 4.0 mg/L. These concentrations lay in the range of the theoretical value of 3.1 mg/L, based on a carbon content of the test item of 14.43 %. The DOC concentration in the other treatments and the blank control were in the same range. Therefore, the determination of the results was based on the nominal concentrations.
Fecundity
Treatment [mg/L] | Total offspring | Surviving parental animals | Mean offspring/surviving parental animal | Inhibition of reproduction [%] |
control | 550 | 8 | 69 | 0 |
1.1 | 792 | 10 | 79 | -15 |
2.2 | 684 | 10 | 68 | 1 |
4.6 | 652 | 10 | 65 | 5 |
10 | 720 | 9 | 80 | -16 |
22 | 909 | 10 | 91 | -32 |
Description of key information
NOEC (21d) >= 5.5 mg/L (AS, nominal) for Daphnia magna (OECD 211)
Key value for chemical safety assessment
Additional information
One study investigating the long-term toxicity of l-Glutamic acid, N-coco acyl derivs., disodium salts (CAS No. 68187-30-4) to aquatic invertebrates is available. The study was performed according to GLP and OECD guideline 211 using Daphnia magna as test organism (Muckle 2016). Nominal concentrations between 1.1 and 22 mg/L were tested, which correspond to test concentrations of 0.275 to 5.5 mg/L based on active matter. Nominal concentrations were confirmed using TOC analysis. No inhibition of reproduction was observed in any treatment. Thus, a 21d-NOEC is determined to be > 5.5 mg AS/L based on nominal concentrations.
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