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Diss Factsheets
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EC number: 201-069-1 | CAS number: 77-92-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- The study was well documented and meets generally accepted scientific principles, but was not conducted in compliance with GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute and subacute toxicological studies of TAKEDA - Citric acid in mice and rats
- Author:
- Yokotani et al.
- Year:
- 1 971
- Bibliographic source:
- J.Takeda Res. Lab. 30 (1) 25-31 (1971)
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Citric acid
- EC Number:
- 201-069-1
- EC Name:
- Citric acid
- Cas Number:
- 77-92-9
- Molecular formula:
- C6H8O7
- IUPAC Name:
- 2-hydroxypropane-1,2,3-tricarboxylic acid
- Details on test material:
- - Name of test material (as cited in study report): TAKEDA-citric acid
- Analytical purity: 99.8% citric acid monohydrate, heavy metals and normal paraffins were present at the concentration below 0.001, and below 0.1ppm, respectively.
Constituent 1
Test animals
- Species:
- other: rat (ICR-JCL) and mice (SD-JCL)
- Sex:
- male
Administration / exposure
- Route of administration:
- intraperitoneal
- Doses:
- 1250 mg/kg, 1042 mg/kg (other doses administered, but not clear from the report what they were)
- No. of animals per sex per dose:
- 6 males
- Control animals:
- not specified
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 940 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 872 - 1 019
- Remarks on result:
- other: mouse
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 725 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 690 - 762
- Remarks on result:
- other: rat
Any other information on results incl. tables
Immediately after administration the animals showed stretching and slow crawling thereafter some laid down on their side. Death of mice given 1250 mg/kg of test compound occurred within 6 to 180 minutes by respiratory and cardiac failures. Some of mice and rats given doses below 1042 mg/kg survived but showed tremor, slight opisthotonus and a slow avoidance reaction to external stimuli within 30 minutes after administration. Some of the animals died by emaciation 2 to 5 days after injection, while others were found to recover within 3 days. At necropsy the surface of the spleen and liver was found to be covered with a thin whitish membrane and there was slight hypertrophy of liver.
Applicant's summary and conclusion
- Conclusions:
- Acute intraperitoneal LD50 values of 940 in mice and 725 mg/kg in rats (males only) were determined in a reliable study conducted according to an appropriate test protocol. The study was not conducted according to GLP.
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