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EC number: 413-860-0 | CAS number: 1187-74-2 ACETONYLBERNSTEINSÄUREESTER; ASE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well conducted GLP compliant study according to international test guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diethyl 4-oxopentane-1,2-dicarboxylate
- EC Number:
- 413-860-0
- EC Name:
- Diethyl 4-oxopentane-1,2-dicarboxylate
- Cas Number:
- 1187-74-2
- Molecular formula:
- C11H18O5
- IUPAC Name:
- 1,4-diethyl 2-(2-oxopropyl)butanedioate
- Details on test material:
- - Name of test material (as cited in study report): acetonyl succinic acid diethyl ester
- Physical state: Liquid
- Analytical purity: 96.1%
- Lot/batch No.: A548702
- Expiration date of the lot/batch: 1992-05-25
- Storage condition of test material: Room temperature, protected from light and humidity
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent from municipal sewage treatment plant: Waterschap de Aa, Schijndel, Netherlands
- Storage conditions: Aeration at 20±1°C
- Preparation of inoculum for exposure: Collected effluent filtered
- Pretreatment: None
- Concentration of sludge: 100µL/L final volume
- Initial cell/biomass concentration:
- Water filtered: yes
- Type and size of filter used, if any: Reverse osmosis followed by activated carbon and ion-exchange cartridges - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Solution containing potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, ammonium chloride, magnesium sulphate, calcium chloride and ferric chloride dissolved in water in accordance with test guidelines
- Additional substrate: None
- Solubilising agent (type and concentration if used): None
- Test temperature:20.2 - 20.5 °C
- pH: 7.3 - 7.8
- pH adjusted: no
- Aeration of dilution water: Yes
- Suspended solids concentration: 100µL/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: oxygen bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration of medium with oil free air until saturation. Medium left for 67 hours at 20±2°C
- Measuring equipment: Oxygen electrode
SAMPLING
- Sampling frequency: Day 0 (test start), 5, 15 and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes
- Other: Positive control (sodium acetate)
STATISTICAL METHODS: None
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- -34
- Sampling time:
- 28 d
- Remarks on result:
- other: 1 mg/L substance
- Parameter:
- % degradation (O2 consumption)
- Value:
- -9
- Sampling time:
- 28 d
- Remarks on result:
- other: 3 mg/L substance
BOD5 / COD results
- Results with reference substance:
- Biodegradation >60% (72%) within 5 days and 85% within 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test susbtance is not readily biodegradable.
The test substance may be toxic to the bacteria in the inoculum - Executive summary:
The test susbtance is not readily biodegradable.
The test substance may be toxic to the bacteria in the inoculum
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