N-(hydroxymethyl)acrylamide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(hydroxymethyl)acrylamide

EC Number:

213-103-2

EC Name:

N-(hydroxymethyl)acrylamide

Cas Number:

924-42-5

Molecular formula:

C4H7NO2

IUPAC Name:

N-(hydroxymethyl)acrylamide

Details on test material:

The test article was identified as CT-444-90E, containing 40-44% N-Methylolacrylamide in water (with < 5% acrylamide and < 3% formaldehyde).
Test Substance: H20 (~50-54%), C4H7O2N (~40-44%), C3H5NO (<5“%), CH2O (<3%), C7H8O2 (~0.003%), Cu.H2O4S (~0.0002%). Carbon content was 23.7%.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of activated sludge: Bergen Co., New Jersey
- Storage conditions: no data given
- Storage length: no data given
- Concentration of sludge: no data given
- Initial cell/biomass concentration: no data given
- Water filtered: no data given
- Type and size of filter used, if any: no data given

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

ca. 1 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

ca. 2 mg/L

Based on:

test mat.

Initial test substance concentration 3

Initial conc.:

ca. 5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Stock solution was prepared by adding 2 g of sample to 1 liter of distilled water. The stock solution was screened to determine if it had a similar
percent carbon content as stated in the formula provided by the supplier. Stock solution was diluted to 100 ppm as carbon after analysis. The diluted
stock was then added to BOD bottles at 3.33 ml, 6.67 ml and 16.65 ml to yield test concentrations of 1 mg, 2 mg and 5 mg as carbon, respectively.
Test solutions were inoculated with a low concentration of microorganisms from a mixed population and kept in closed bottles in the dark at a constant
temperature of 20 ± 1° C. The activated sludge bacteria was from Bergen Co., New Jersey.
The degradation was followed by oxygen analyses with the YSI Dissolved Oxygen Analyzer 54A over a 28-day period.
Degradability was based on a comparison of readings of actual oxygen demand to the theoretically expected oxygen demand.
A parallel control with inoculum, but without test material, was run as a blank correction factor.
The procedure was validated by means of a reference substance (aniline, 2 mg/l) of known biodegradability.

Reference substance

Reference substance:

aniline

Remarks:

2mg/L

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

15 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

ca. 16.7

Sampling time:

15 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

ca. 51.9

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Aniline: day 5 = 18.5 %, day 15 = 66.7 %, day 28 = 98.1 %

Any other information on results incl. tables

Sample conc. (mg/l)	Degradation at:
	5 days	15 days	28 days
1	0%	0%	40.7%
2	0%	16.7%	51.9%
5	0%	14.8%	40.7%
Average	0%	10.5%	44.3%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

Under the test conditions, the test substance was not readily biodegradable (51.9% in 28 days)