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Diss Factsheets
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EC number: 200-663-8 | CAS number: 67-66-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Six valid studies using static, semi-static or flow-through systems and investigating the acute toxicity to different freshwater fish species have been identified. Only one study on acute toxicity to a marine fish species has been found. The 96-hours LC50 value in the most sensitive freshwater fish species was 18 mg/L. The LC50 value found for the only tested marine fish species was 28 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 18 mg/L
Marine water fish
Marine water fish
- Effect concentration:
- 28 mg/L
Additional information
The experiments carried out with different freshwater fish species had testing periods ranging from 96 hours to 14 days. The LC50 values found in the studies range from 18 mg/L for Oncorhynchus mykiss to 171 mg/L for Pimephales promelas (Röderer 1990, Anderson and Lustry 1980, Könemann 1981, Mayes et al. 1983, Geiger et al. 1990). Thus, the rainbow trout Oncorhynchus mykiss tends to represent the most sensitive freshwater fish species. The 96 hours LC50 value of 18 mg/L was obtained with daily analytical monitoring of the chloroform concentration in the test water and a flow-through toxicant delivery system (Anderson and Lustry 1980) and is retained for the risk assessment.
Only one study delivers information about the acute toxicity of chloroform to the saltwater fish species Limanda limanda. Very few data are given with regard to the test substance and the conditions of the test. However, the 96 -hours LC50 value given for chloroform is 28 mg/L (Pearson et al. 1975), which is in good agreement with that of the most sensitive freshwater species. Thus, the study tends to show that the acute toxicity of chloroform to fish species is rather similar in freshwater and saltwater systems.
In conclusion, the LC50 value of 18 mg/L is used to characterise the short-term toxicity of chloroform to fish and considered to be representative of both, the freshwater and the saltwater systems.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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