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EC number: 201-279-3 | CAS number: 80-43-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-12-07 to 21-04-2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.400, 0.200, 0.100 0.0500, 0.0250 mg/L (nominal)
- Sampling method: Samples were taken 6 times (3 times repeated before and after changing the water) during the exposure period for all test plots (one per test vessel), and after removing food from the samples before changing the water by centrifugation. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the necessary amount of test substance to achieve a concentration of 10,000 mg/L was dissolved in DMSO. The test solution was
prepared by adding a necessary amount of the undiluted test solution to the dilution water put in a preparation vessel while stirring .
- Controls: 10 replicates each of dilution water and solvent control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethyl sulfoxide (DMSO)
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 µl/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea ,Ohmijinko (japanese), (Daphnia magna)
- Strain/clone: Clone A
- Source: Sheffield University. Sheffield S10 2Uq, United Kingdom
- Feeding during test
- Food type: Chlorella vulgaris
- Amount: 0.1-0.2 mg C (organic carbon) per day per Daphnia
ACCLIMATION
- Acclimation period: 2000.01.03-2000.31.03
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Chlorella vulgaris. 0.1-0.2 mg C per day per Daphnia
- Health during acclimation (any mortality observed): Death rate was 5 % or less
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 37.0-48.4 mg/L as CaCO3
- Test temperature:
- 20.0-20.5 °C
- pH:
- 7.4-7.9
- Dissolved oxygen:
- 8.3-8.8 mg/L
- Nominal and measured concentrations:
- 0.0250, 0.0500, 0.100, 0.200, 0.400 mg/L (nominal)
0.187, 0.0331, 0.06, 0.117, 0.247 mg/L (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL beaker:
- Renewal rate of test solution (frequency/flow rate): once per day
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Kurume City tap water
- Metals: Tin, 0.02 mg/L
- Chlorine: 0.02 mg/L or lower
- Alkalinity: 27.5 mg/L
- Ca/Mg ratio: 2.62
- Conductivity: 146 µS/cm
- Culture medium different from test medium: No
- Intervals of water quality measurement: Water quality test performed on Februrary 8, 2000
OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- Light intensity: 1200 lux or lower
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Reproduction and mortality. Every day, the number of surviving and immobilized Daphnia were recorded. Every day, the number of live and dead juveniles, occurences of aborted eggs and diapause eggs were recorded.
VEHICLE CONTROL PERFORMED: Yes
RANGE-FINDING STUDY: Yes, but results were not reported. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.231 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 95% CL: 0.209 - 0.265 mg/L
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.195 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 95% CL: 0.177 - 0.221 mg/L
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 0.195 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: mortality of parental Daphnia
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 0.208 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: mortality oft parental Daphnia
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.177 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks on result:
- other: average cumulative number of brood
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.247 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks on result:
- other: average cumulative number of brood
- Details on results:
- - Mortality of parent animals: No mortalities were observed in any of the control plots. Among all test plots, only the concentration at 0.247 mg/L showed any mortalities. On day 4, seven mortalities were observed and then one further mortality occured on day 17, bringing the total number of mortalities to 8 after 21 days. The other concentration plots did not show any deaths.
- No. of offspring produced per day per female: See table 1
- Time to first brood release or time to hatch: The mean brood release time for all concentrations and controls was 8 days, except the test concentration at 0.267 mg/L, which took on average 8.7 days for brood production.
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- 48-hour median immobilization concentration (EiC50) by the reference material (potassium dichromate, reagent chemical of highest quality, Wako Pure Chemical Industries, Ltd.) = 0.141 mg/L [EiC50 after January 1998 in Kurume Business Office: Within the range of 0.128~0.234 mg/L (n = 5).]
- Reported statistics and error estimates:
- Calculation of EC50:
EC50 was calculated by the moving average method. The calculation was made by applying the maximum average cumulative number of brood (rounding up digits to the right of the decimal point) over all the test plots to the number of specimens under testing, and the average cumulative number of brood in each concentration plot to the number of survivers. EC50 of the 14th and 21st days were calculated, and their 95 % confidence limits are also shown.
Calculation of maximum no observed effect concentration (NOEC) and lowest observed effective concentration (LOEC):
The cumulative number of brood per one parent for each test vessel was calculated, after performing homoscedastic testing by the Bartlett method.
Significant difference between each concentration plot and the solvent control plot was determined by one-way analysis of variance and Dunnett's multiple comparison method.
Also, the significant difference between each concentration plot and the control plot for the number of dead parental Daphnia was determined by the Kruskal-Wallis rank sum test and the nonparametric multiple comparison method (Dunnett). The nonparametric multiple comparison method (Scheffé) was used for the primiparous day. NOEC and LOEC were determined including the states of observed parents and produced juveniles in this result. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 21-day-chronic toxicity of dicumylperoxide to Daphnia magna was studied under static renewal conditions. The parental 21-day LC50 based on mortality was 0.195 mg/L, the 21-day EC50 for reproduction was 0.231 mg/L. The 21-day NOEC based on average cumulative number of brood was 0.117 mg/L, the 21-day LOEC was 0.247 mg/L. Sublethal effects were observed in the 0.247 mg/L plot.
Production of offspring in the treated groups indicated that dicumylperoxide had an effect on reproduction at concentrations greater than 0.117 mg/L. The most sensitive end point was reproduction. - Executive summary:
The 21-day-chronic toxicity of dicumylperoxide to Daphnia magna was studied under static renewal conditions. Daphnids were exposed to control, solvent control, and test chemical at measured concentrations of 0.187, 0.0331, 0.0600, 0.117, 0.247 mg/L. The parental 21-day LC50 based on mortality was 0.195 mg/L, the 21-day EC50 for reproduction was 0.231 mg/L. The 21-day NOEC based on average cumulative number of brood was 0.117 mg/L, the 21-day LOEC was 0.247 mg/L. The sublethal effects included were body colour brightening and growth inhibition in the 0.247 mg/L plot compared with the control plot, besides lethargy, immobility, and activity drop were observed. No symptoms were observed in the other concentration plots. Dead larvae were observed in all the test plots, and the 0.247 mg/L plot had somewhat more than the control plot. Fallen eggs were also observed in all of the test plots, the 0.117 mg/L showed a statistically significant difference.
Production of offspring in the treated groups indicates that dicumylperoxide had an effect on the reproduction at concentrations greater than 0.117 mg/L. The most sensitive endpoint was reproduction.
This study is classified as reliable without restriction and satisfies the guideline requirements for a chronic toxicity study with freshwater invertebrates.
Results synopsis
Test organism age: First instar
Test type (static renewal): OECD guideline 211 (Daphnia magna reproduction test)
21-day EC50 (reproduction): 0.231 mg/L (95% CL: 0.209–0.265 mg/L)
21-day LC50 (parental mortality): 0.195 mg/L
21-day NOEC (average cumulative number of brood): 0.117 mg/L
21-day LOEC (average cumulative number of brood): 0.247 mg/L
Endpoint(s) effected: Reproduction, parental mortality
Reference
Table 1: Mean cumulative number of juveniles produced per adult (Σ F1/P) during exposure to di-α-cumyl peroxide and average juvenile produced per day over 21 days.
Measured concentration (mg/L) |
Exposure time (days) |
|||||||
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
|
Control |
13.9 |
NC |
NC |
40.3 |
NC |
NC |
71.2 |
74.1 |
Solvent control |
13.5 |
NC |
NC |
35.9 |
37.1 |
NC |
69.4 |
72.6 |
0.0187 |
14.5 |
NC |
NC |
42.2 |
NC |
NC |
75.0 |
75.6 |
0.0331 |
14.4 |
NC |
NC |
36.9 |
37.6 |
NC |
69.8 |
NC |
0.600 |
13.3 |
NC |
NC |
38.3 |
NC |
NC |
72.3 |
72.6 |
0.117 |
11.2 |
NC |
NC |
35.2 |
NC |
NC |
65.9 |
66.1 |
0.247 |
2.5 |
4.5 |
NC |
14.0 |
23.5 |
NC |
NC |
39.0 |
* table begins on day 8 when the first juveniles appeared
*NC = no change from the previous day´s value.
Table 1 continued:
Measured concentration (mg/L) |
Exposure time (days) |
Average number produced per day |
|||||
16 |
17 |
18 |
19 |
20 |
21 |
||
Control |
74.1 |
NC |
103 |
NC |
NC |
128 |
6.10 |
Solvent control |
72.6 |
NC |
104 |
NC |
NC |
129 |
6.14 |
0.0187 |
75.6 |
NC |
108 |
NC |
NC |
134 |
6.38 |
0.0331 |
72.0 |
NC |
107 |
NC |
NC |
131 |
6.24 |
0.0600 |
75.8 |
NC |
106 |
NC |
NC |
133 |
6.33 |
0.117 |
68.3 |
NC |
96.4 |
NC |
NC |
120 |
5.71 |
0.247 |
39.0 |
NC |
52.0 |
53.0 |
NC |
60.0 |
2.86 |
The average cumulative number of brood per parental Daphnia in the 0.247 mg/L plot was 60, showing a significant difference compared with that in the solvent control plot. The average cumulative numbers of brood per parental Daphnia in the 0.117~0.0187 mg/L plots were 120, 133, 131, and 134, respectively, almost the same as those in the solvent control plot and the control.
Size and state of parental Daphnia
During the exposure period, body color brightening and growth inhibition were observed in the 0.247 mg/L plot compared with the control plot, besides lethargy, immobility, and activity drop were observed. No symptoms were observed in the other concentration plots.
Occurrence of diapause eggs, etc.
Throughout the entire exposure period, no occurrence of diapause eggs was observed in any test plot.
Dead larvae were observed in all the test plots, and the 0.247 mg/L plot had somewhat more than the control plot. Fallen eggs were also observed in all of the test plots, the 0.117 mg/L showed a statistically significant difference.
Description of key information
21-day NOEC (average cumulative number of brood): 0.117 mg/L
Endpoint(s) effected: ReproductionKey value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.117 mg/L
Additional information
The 21-day chronic toxicity of dicumyl peroxide to Daphnia magna was studied under static renewal conditions. The parental 21-day LC50 based on mortality was 0.195 mg/L, the 21-day EC50 for reproduction was 0.231 mg/L. The 21-day NOEC based on average cumulative number of brood was 0.117 mg/L, the 21-day LOEC was 0.247 mg/L. Sublethal effects were observed in the 0.247 mg/L plot (body colour brightening and growth inhibition, besides lethargy, immobility, and activity drop). No symptoms were observed in the other concentration plots. Dead larvae were observed in all the test plots, and the 0.247 mg/L plot had somewhat more than the control plot. Fallen eggs were also observed in all of the test plots, the 0.117 mg/L showed a statistically significant difference. Production of offspring in the treated groups indicated that dicumyl peroxide had an effect on the reproduction at concentrations greater than 0.117 mg/L. The most sensitive end point was reproduction.
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