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Diss Factsheets
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EC number: 203-401-0 | CAS number: 106-47-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication which meets basic scientific principles
Data source
Reference
- Reference Type:
- publication
- Title:
- Testing for the toxicity of chemicals with Tetrahymena pyriformis
- Author:
- Yoshioka Y, Ose Y, and Sato T
- Year:
- 1 985
- Bibliographic source:
- Sci. Total Environ. 43, 149-157
Materials and methods
- Principles of method if other than guideline:
- other: cell multiplication inhibition test
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-chloroaniline
- EC Number:
- 203-401-0
- EC Name:
- 4-chloroaniline
- Cas Number:
- 106-47-8
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 4-chloroaniline
- Details on test material:
- - Name of test material (as cited in study report): p-Chloroaniline
- Analytical purity: "analytical grade"
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- - Stock solution of test substance was added to the sterile medium to provide a concentration ratio
of 1.8 in 10 ml of 2 % proteose peptone.
- The solutions were then inoculated with 0.2 ml of the pre-cultured T. pyriformis and cultivated at
a temperature of 30 °C for 24 h without agitation.
Test organisms
- Test organisms (species):
- Tetrahymena pyriformis
- Details on inoculum:
- - Tetrahymena pyriformis was preserved in a sterile medium (of 2 % proteose peptone at a temperature of 20 °C) which was renewed at 2-4 week intervals.
- Tetrahymena pyriformis was pre-cultured at a temperature of 30 °C for 24 h.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Test temperature:
- 30 °C
Results and discussion
Effect concentrations
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Applicant's summary and conclusion
- Executive summary:
A cell-multiplication-inhibition test on the acute toxicity of 4-chloroaniline to Tetrahymena pyriformis was performed for a test period of 24 hours. An EC50 value of 10 mg/l was reported (Yoshioka et al., 1985).
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