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EC number: 939-529-5 | CAS number: 1471313-51-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-01-23 to 2013-03-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study, no deficiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acid chlorides, C12-18 (even numbered) and C18 unsatd., reaction products with sodium N-methyltaurinate
- EC Number:
- 939-529-5
- Cas Number:
- 1471313-51-5
- Molecular formula:
- not applicable
- IUPAC Name:
- Fatty acid chlorides, C12-18 (even numbered) and C18 unsatd., reaction products with sodium N-methyltaurinate
- Details on test material:
- - Name of test material (as cited in study report): Sodium methyl cocoyl taurate
- Physical state: Slightly yellowish coarse powder, solid
- Stability under test conditions: stable
- Storage condition of test material: Room temperature, protected from light, in original container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.500 – 1.00 – 2.00 – 4.00 – 8.00 mg/L
- Sampling method: All concentration levels of the test item and the control were analysed by LC-MS/MS at the start of the exposure intervals on days 7, 9, 14 (0 h) and at the end of the exposure intervals on days 9, 12, 16 (48 or 72 h).
For the analyses of the old media, separate replicates without daphnids and algae were prepared and stored under test conditions until sampling.
- Sample storage conditions before analysis: All original samples were stored room temperature until sample preparation. Prepared samples were stored at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (8.00 mg/L of the test item were weighed out) was freshly prepared with dilution water at the start of the test period and at every water renewal day.Dispersion treatment was agitation.
- Eluate: Dilution water
- Differential loading: 0.500 – 1.00 – 2.00 – 4.00 – 8.00 mg/L
- Controls: 10 replicates of dilution water without test item.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: Clone 5
- Justification for species other than prescribed by test guideline: Daphnia magna STRAUS is recommended in the guideline
- Source: Own breeding (Origin: Institut fuer Wasser- Boden- und Lufthygiene)
- Age of parental stock (mean and range, SD): > 14 days
- Feeding during test
- Food type: Mix of Pseudokirchneriella subcapitata and Desmodesmus subspicatus
- Amount: 0.2 mg C/daphnia per day
- Frequency: renewals 3 times per week
ACCLIMATION
- Acclimation period: 2 h in dilution water
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: During acclimation the daphnids were not fed
- Feeding frequency: None
- Health during acclimation (any mortality observed): Healthy
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: The parent animals were removed from the culture medium and the juveniles collected over a sieve and flushed into fresh medium
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- Not observed
Test conditions
- Hardness:
- Total Hardness [mg/L] as CaCO3
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-02-06 2013-02-08 2013-02-15 2013-02-18 2013-02-20 2013-02-22
8.00 173 171 178 155 166 146
Control 162 164 178 171 169 148 - Test temperature:
- Temperature [°C]
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-02-06 2013-02-08 2013-02-15 2013-02-18 2013-02-20 2013-02-22
8.00 21.2 20.6 19.9 20.3 21.0 20.9
Control 19.7 20.5 19.6 20.3 20.2 21.0 - pH:
- pH-Values
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-02-06 2013-02-08 2013-02-15 2013-02-18 2013-02-20 2013-02-22
8.00 7.81 7.60 8.74 7.78 7.82 7.51
Control 8.14 7.66 8.89 7.84 8.34 7.64 - Dissolved oxygen:
- Dissolved Oxygen Concentration [mg/L]
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-02-06 2013-02-08 2013-02-15 2013-02-18 2013-02-20 2013-02-22
8.00 8.59 7.16 8.69 7.82 8.90 6.76
Control 8.75 6.75 10.29 7.88 8.96 7.85 - Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to "Any other information on materials and methods"
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: 100 mL glass beaker, filled with 50 mL test solution
- Aeration: No
- Renewal rate of test solution (frequency): 3 x per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control and pH-control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4, according to ELENDT (1990), modified to a total hardness of > 140 mg CaCO3/L.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark per day
- Light intensity: Max. 20 µE*m-2*s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Adult mortality: daily
- Number of juveniles: daily
- Stillborn juveniles and aborted eggs: daily
- Appearance of first brood
- Intrinsic rate of natural increase: test end
- Growth (total length and dry weight): test end
RANGE-FINDING STUDY
Based on the results of an acute immobilisation test (NOACK Lab-ID: 120425CS/DAI15129) conducted at the test facility, the definitive reproduction test was carried out with 5 concentration levels of the test item in the range of 0.500 to 8.00 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- is UVCB means 100% act.ingr.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals:
The test item did not induced biologically significant adult mortality of > 20 % at any of the tested concentration levels during the test period of 21 days.
At the concentration level of 8.00 mg/L, the adult mortality was 10 %. At the concentration levels of 0.500 to 4.00 mg/L and in the control, all parental daphnids survived until the end of the test after 21 days. The EC10 for the adult mortality was deduced directly from the observation data to be 8.00 mg/L. An EC50 could not be determined, because adult mortality of > 50 % was not observed.
- No. of offspring produced per day per female:
The average number of living juveniles per surviving parental daphnid at the end of the test after 21 days was 93 in the control group. The reproductive output was statistically significant increased in comparison to the control at the concentration level of 8.00 mg/L (One Way Analysis of Variance, DUNNETT’S method, p = 0.05). At the concentration level of 8.00 mg/L, an increased number of living juveniles per brood were observed and a reduced number of broods at two of nine surviving daphnids. Furthernore, the calculated coefficient of variation of the number of living offspring of 29 % for the concentration level 8.00 mg/L was biologically noticeable increased.
At the concentration levels of 0.500 to 4.00 mg/L, the reproductive output was comparable to the reproductive output at the control.
The EC10 for the increase of the reproductive output was assed to be within the range of 4.00 to 8.00 mg/L. An EC50 could not be calculated, because the increase of the reproductive output was limited to a maximum of 33 %.
- Body length and weight of parent animals:
The mean values of the body length of the surviving parental daphnids in the tested concentration levels of 0.500 to 8.00 mg/L were determined to be in the range of 5.28 to 5.60 mm per daphnid, 5.45 mm per daphnid in the control group.
The mean dry body weight was determined to be in the range of 1.21 to 1.42 mg per daphnid in the concentration levels 0.500 to 8.00 mg/L and 1.49 mg per daphnid in the contro
- Type and number of morphological / behavioural abnormalities:
please refer to the respective table
- Time to first brood release or time to hatch:
The first brood was released between days 8 and 10 by all parental daphnids of the control and the concentration levels of 0.500 to 4.00 mg/L. At the concentration level of 8.00 mg/L, one of nine surviving daphnids released the first brood noticeably delayed on day 18.
Four broods were produced by the surviving parental daphnids at the tested concentration levels of 0.500 to 4.00 mg/L and in the control. At the concentration level of 8.00 mg/L, two of nine surviving daphnids released less than four broods - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (24 h) = 1.69 (CI 1.41 - 2.12) mg/L - Reported statistics and error estimates:
- The NOEC and the LOEC, based on the increase of the reproductive output, were deduced directly from the results. Significant deviations were determined in comparison to the control using statistical standard procedures as normality test, equal variance test and analysis of variance (parametric ANOVA, DUNNETT’S method).
Prior to running the parametric ANOVA, a normality test and an equal variance test were performed. P-values of the normality and equal variance tests should be 0.05. Actually, the P-value was < 0.05 and therefore the normality test failed for the statistical evaluation of the reproduction rates and the intrinsic rates of natural increase. Furthermore, the equal variance test for the evaluation of the reproduction rates failed (P value < 0.05).
The a-value (acceptable probability of incorrectly concluding that there is a difference) was a=0.05.
For the determination of significant deviations of the reproduction rates and the intrinsic rates of natural increase, One Way Analysis of Variance, DUNNETT’S method (p = 0.05) was used.
The coefficients of variation around the mean number of living offspring produced per parent in the control and the test groups were evaluated.
The EC10-values for the increase of the reproductive output and the adult mortality after 21 days were deduced directly from the observation data without mathematical calculation.
The EC50-value for the reference item was calculated by sigmoidal dose-response regression. The 95 % confidence limits for the EC-values were calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad prism5.
An EC50-value for the increase of the reproductive output or the adult mortality after 21 days could not be calculated because effects > 50 % were not observed.
Any other information on results incl. tables
Number of Juveniles in the Control and Test Groups after 21 Days
Nominal test item conc. [mg/L] |
No. of parents prod. juv. |
Mean no. of Juveniles per parent producing juveniles |
Comparison versus control |
||||||||||||||
Number of living juveniles in replicate no. |
Total no. |
CV |
|||||||||||||||
Inc. |
Stat. |
||||||||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
å |
N |
MV ± SD |
[%] |
[%] |
|||
8.00 |
141 |
149 |
140 |
92 |
45 |
124 |
167 |
137 |
119 |
-- |
1114 |
9 |
124 ± |
36 |
29* |
33 |
yes |
4.00 |
101 |
114 |
102 |
86 |
79 |
84 |
82 |
85 |
97 |
92 |
922 |
10 |
92 ± |
11 |
12 |
-1 |
no |
2.00 |
89 |
111 |
101 |
115 |
104 |
101 |
119 |
93 |
93 |
93 |
1019 |
10 |
102 ± |
10 |
10 |
10 |
no |
1.00 |
77 |
111 |
99 |
87 |
98 |
101 |
76 |
85 |
107 |
91 |
932 |
10 |
93 ± |
12 |
13 |
1 |
no |
0.500 |
95 |
108 |
97 |
92 |
92 |
98 |
95 |
82 |
87 |
93 |
939 |
10 |
94 ± |
7 |
7 |
1 |
no |
Control |
80 |
89 |
103 |
90 |
94 |
103 |
85 |
89 |
103 |
93 |
929 |
10 |
93 ± |
8 |
9 |
— |
— |
First Appearance of Living Juveniles in the Individual Groups
Nominal test item concentration [mg/L] |
Day of first appearance of living juveniles |
First |
Mean |
|||||||||
in replicate no. |
appearance |
number |
||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
mean day |
of broods |
|
8.00 |
8 |
9 |
8 |
8 |
181) |
9 |
8 |
8 |
9 |
-- |
9.4 |
3.562) |
4.00 |
8 |
8 |
9 |
8 |
8 |
8 |
8 |
9 |
8 |
9 |
8.3 |
4.00 |
2.00 |
9 |
8 |
9 |
9 |
8 |
9 |
9 |
8 |
8 |
9 |
8.6 |
4.00 |
1.00 |
10 |
8 |
9 |
8 |
9 |
8 |
8 |
8 |
9 |
8 |
8.5 |
4.00 |
0.500 |
8 |
9 |
9 |
9 |
8 |
9 |
9 |
9 |
9 |
8 |
8.7 |
4.00 |
Control |
8 |
8 |
8 |
8 |
8 |
8 |
9 |
8 |
9 |
8 |
8.2 |
4.00 |
Relative Number of Dead (Stillborn + Aborted Eggs) to Total Number of Juveniles
Nominal test item concentration [mg/L] |
Number of juveniles |
Percentage of dead juveniles#) [%] |
||
Dead |
Alive |
Total |
||
8.00 |
45 |
1114 |
1159 |
4 |
4.00 |
55 |
922 |
977 |
6 |
2.00 |
48 |
1019 |
1067 |
5 |
1.00 |
10 |
932 |
942 |
1 |
0.500 |
5 |
939 |
944 |
1 |
Control |
0 |
929 |
929 |
0 |
Mortality [%] of the Adult Daphnids after 7, 14 and 21 Days of Exposure
(n = 10)
Nominal Test Item Concentration [mg/L] |
Adult Mortality [%] |
||
7 days |
14 days |
21 days |
|
8.00 |
10 |
10 |
10 |
4.00 |
0 |
0 |
0 |
2.00 |
0 |
0 |
0 |
1.00 |
0 |
0 |
0 |
0.500 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
Total Body Length of the Parental Daphnids
Nominal test item concentration [mg/L] |
Total length of the parent animals [mm] |
MV |
N |
Dry weight |
||||||||||
Replicate no. |
||||||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
[mm] |
S |
MV |
||
8.00 |
5.00 |
5.75 |
5.50 |
5.50 |
5.25 |
5.00 |
5.50 |
5.75 |
5.50 |
-- |
5.42 |
9 |
10.9 |
1.21 |
4.00 |
5.25 |
5.25 |
5.00 |
5.25 |
5.50 |
5.50 |
5.25 |
5.75 |
5.50 |
5.50 |
5.38 |
10 |
13.6 |
1.36 |
2.00 |
5.75 |
5.75 |
5.50 |
5.50 |
6.00 |
5.50 |
5.50 |
5.75 |
5.75 |
5.00 |
5.60 |
10 |
14.2 |
1.42 |
1.00 |
5.25 |
5.00 |
5.50 |
5.25 |
5.00 |
5.25 |
5.50 |
5.50 |
5.25 |
5.25 |
5.28 |
10 |
13.4 |
1.34 |
0.500 |
5.50 |
5.00 |
5.50 |
5.25 |
5.25 |
5.25 |
5.25 |
5.25 |
5.75 |
5.00 |
5.30 |
10 |
13.9 |
1.39 |
Control |
5.75 |
5.25 |
5.25 |
5.50 |
5.50 |
5.75 |
5.50 |
5.25 |
5.50 |
5.25 |
5.45 |
10 |
14.9 |
1.49 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The statistically significant increase of the reproductive output at the concentration level of 8.00 mg/L and the noticeably increased coefficient of variation of the number of living offspring were the most sensitive effects in this study. At the concentration level of 8.00 mg/L, an increased number of living juveniles per brood was observed.
Based on the significant increase of the reproductive output and the noticeably increased coefficient of variation of the number of living offspring at the concentration level of 8.00 mg/L, the No Observed Effect Concentration (NOEC) after 21 days was assessed at 4.00 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 8.00 mg/L.
Based on the nominal concentrations of the active ingredient, the NOEC was 2.89 mg a.i./L and the LOEC was 5.78 mg a.i./L.
The EC10 for the increase of the reproductive output was assed to be within the range of 4.00 to 8.00 mg/L, which corresponds to 2.89 – 5.78 mg a.i./L. An EC50 could not be calculated, because the increase of the reproductive output was limited to a maximum of 33 %.
At the concentration level of 8.00 mg/L, the adult mortality was 10 %. Therefore, the EC10 for the adult mortality was assessed at 8.00 mg/L, which corresponds to 5.78 mg a.i./L. An EC50 could not be determined, because adult mortality of > 50 % was not observed.
Endpoints assessed Sodium methyl cocoyl taurate
Nominal concentration
of the test item
[mg/L] Nominal concentration
of the active ingredient
[mg a.i./L]
EC10 Reproduction 4.00 mg/L < EC10 < 8.00 2.89 mg/L < EC10 < 5.78
EC10 Adult Mortality 8.00 5.78
NOECReproduction 4.00 2.89
LOECReproduction 8.00 5.78 - Executive summary:
The Daphnia magna Reproduction Test (semi-static, 21 d) of the test item Sodium methyl cocoyl taurate (batch number: DEB4003323) was conducted according to OECD 211 (2008) from 2013-01-23 to 2013-03-01, with the definitive exposure phase from 2013-02-06 to 2013-02-27, at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany.
Test species was Daphnia magna STRAUS (Clone 5). Ten daphnids, individually held, were used per concentration level, and control. At test start the daphnids were 2 to 24 hours old. The study was carried out undersemi-static conditions with renewal of the test solutions three times per week. Aim of the Daphnia Reproduction Test over 21 days was to assess effects on the reproduction capacity and other test item-related effects or parameters such as time of production of first brood, adult mortality, intrinsic rate of natural increase, occurrence of aborted eggs and stillborn juveniles and body length of the parental daphnids.
Nominal concentrations of Sodium methyl cocoyl taurate were selected as follows: 0.500 – 1.00 – 2.00 – 4.00 – 8.00 mg/L. This corresponds to the nominal concentrations of the active ingredient of 0.362 – 0.723 – 1.45 – 2.89 – 5.78 mg a.i./L based on the nominal content of 72.3 % (w/w).
The concentrations of the test item Sodium methyl cocoyl taurate were analytically verified by LC-MS/MS of samples taken at the start of the exposure intervals on days 7, 9, 14 (0 h) and at the end of the exposure intervals on days 9, 12, 16 (48 or 72 h) of all concentration levels and the control. The main component of the active ingredient (C12 part) was analytically verified. Details of the analytical method are presented in part 11.
The measured concentrations of the active ingredient at the start of the exposure intervals were in the range of 97 to 117 % of the nominal values. At the end of the exposure intervals (48 or 72 h), the measured concentrations of the active ingredient were in the range of 92 to 108 % of the nominal values. This indicates, that the test item concentrations were successfully maintained for the duration of the test.
The endpoints, based on the nominal concentrations of the test item and the active ingredient, are presented in Table 1.
· The average number of living juveniles per surviving parental daphnid at the end of the test after 21 days was 93 in the control group. The reproductive output was statistically significant increased in comparison to the control at the concentration level of 8.00 mg/L (One Way Analysis of Variance, Dunnett’smethod, p = 0.05). At the concentration level of 8.00 mg/L, an increased number of living juveniles per brood were observed and a reduced number of broods at two of nine surviving daphnids. Furthernore, the calculated coefficient of variation of the number of living offspring of 29 % for the concentration level 8.00 mg/L was biologically noticeable increased.
At the concentration levels of 0.500 to 4.00 mg/L, the reproductive output was comparable to the reproductive output at the control.
The EC10 for the increase of the reproductive output was assed to be within the range of 4.00 to 8.00 mg/L. An EC50 could not be calculated, because the increase of the reproductive output was limited to a maximum of 33 %.
· The coefficient of variation of the number of living offspring produced per parent was 9 % in the control. At the concentration levels of 0.500 to 4.00 mg/L, the coefficient of variation was in the range of 7 to 13%. The calculated coefficient of variation of 29 % for the concentration level 8.00 mg/L was biologically noticeable increased. A coefficient of variation above 25 % is regarded to be biologically noticeable
· Based on the significant increase of the reproductive output and the noticeably increased coefficient of variation of the number of living offspring at the concentration level of 8.00 mg/L, the No Observed Effect Concentration (NOEC) after 21 days was assessed at 4.00 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 8.00 mg/L.
· The first brood was released between days 8 and 10 by all parental daphnids of the control and the concentration levels of 0.500 to 4.00 mg/L. At the concentration level of 8.00 mg/L, one of nine surviving daphnids released the first brood noticeably delayed on day 18.
Four broods were produced by the surviving parental daphnids at the tested concentration levels of 0.500 to 4.00 mg/L and in the control. At the concentration level of 8.00 mg/L, two of nine surviving daphnids released less than four broods· The intrinsic rates of natural increase (IR) of the surviving parental daphnids accounting for generation time and number of offspring were used for calculation of population growth and maintenance. The intrinsic rates of natural increase determined for the surviving daphnids at the concentration levels of 0.500 to 8.00 mg/L were comparable to the IR at the control (One Way Analysis of Variance, Dunnett’s method, p = 0.05).
· No stillborn juveniles or aborted egg were observed in the control group during the exposure period of 21 days. Related to the total number of produced juveniles (dead + alive) the percentage of dead juveniles was in the range of 1 to 6 % in the tested concentration levels of 0.500 to 8.00 mg/L.
· The test item did not induced biologically significant adult mortality of > 20 % at any of the tested concentration levels during the test period of 21 days.At the concentration level of 8.00 mg/L, the adult mortality was 10 %. At the concentration levels of 0.500 to 4.00 mg/L and in the control, all parental daphnids survived until the end of the test after 21 days. The EC10for the adult mortality was deduced directly from the observation data to be 8.00 mg/L. An EC50 could not be determined, because adult mortality of > 50 % was not observed.
· The mean values of the body length of the surviving parental daphnids in the tested concentration levels of 0.500 to 8.00 mg/L were determined to be in the range of 5.28 to 5.60 mm per daphnid, 5.45 mm per daphnid in the control group.
The mean dry body weight was determined to be in the range of 1.21 to 1.42 mg per daphnid in the concentration levels 0.500 to 8.00 mg/L and 1.49 mg per daphnid in the control
· No males or ephippia (winter eggs) were observed in the control or in the test groups.
· Water quality parameters as pH-value, dissolved oxygen, water hardness and temperature were determined to be within the acceptable limits.
IMPORTANT NOTE TO AVOID CONFUSION:
As the registration substance is a UVCB this means that the test item is 100% active ingredient.
A summary of all test item related effects and the assessed endpoints based on the nominal concentrations of the test item is given in Table 1.
Table 1: Test Item Related Effects on the Reproduction (NOEC, LOEC and EC10)
(based on the nominal concentrations of the test item )
Effects
Sodium methyl cocoyl taurate
Nominal concentrations of the test item [mg/L]
Control
0.500
0.362
1.00
0.723
2.00
1.45
4.00
2.89
8.00
5.78
Mean Number of Living Juveniles per
Producing Parent (Reproduction Rate ± SD)
93 ± 8
94 ± 7
93 ± 12
102 ± 10
92 ± 11
124 ± 361)
Coefficient of Variation of the Mean Number of Juveniles per Producing Parent [%]
9
7
13
10
12
292)
Mean Number of Broods
4.00
4.00
4.00
4.00
4.00
3.563)
Appearance of First Brood [Mean Day]
8.2
8.7
8.5
8.6
8.3
9.44)
Mean Intrinsic Rates of Natural Increase
0.47
0.46
0.46
0.47
0.47
0.46
Percentage of Dead Juveniles Related to the Total Number of Juveniles [%]
0
1
1
5
6
4
Parental Daphnids: Mean Dry Weight [mg]
1.49
1.39
1.34
1.42
1.36
1.21
Parental Daphnids: Mean Body Length [mm]
5.45
5.30
5.28
5.60
5.38
5.42
Adult Mortality after 21 Days [%]
0
0
0
0
0
10
EC10 Reproduction
4.00 mg/L EC10 Reproduction 8.00 mg/L
EC10 Adult mortality
8.00 mg/L
NOECReproduction
4.00 mg/L
LOECReproduction
8.00 mg/L
SD = standard deviation
1) = statistical significance (One Way Analysis of Variance, Dunnett’smethod, p = 0.05)
2) = biologically noticeable coefficient of variation (> 25 %)
3) = reduced number of broods at two of nine surviving daphnids (less than 4 broods)
4) = delayed appearance of the 1st brood on day 18 at one of nine surviving daphnids
a.i. = active ingredient
· In order to prove the validity of the test system and test conditions at the test facility, an acute immobilisation test according to DIN 38412 L 11 is monthly carried out with potassium dichromate as reference item.
The EC50 of the reference item was 1.69 mg/L (95% confidence limits: 1.41 - 2.12 mg/L) after 24 hours and therefore within the prescribed concentration range of 1.0 - 2.5 mg/L of quality criteria according to AQS P 9/2 (05/1996) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.
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