Registration Dossier
Registration Dossier
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EC number: 296-866-4 | CAS number: 93164-85-3
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-03-3 / 2010-04-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to the OECD Guideline and it is GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Amines, C20-22-alkyldimethyl
- EC Number:
- 296-866-4
- EC Name:
- Amines, C20-22-alkyldimethyl
- Cas Number:
- 93164-85-3
- Molecular formula:
- CnH2nN(CH3)2 n: from 18 to 22
- IUPAC Name:
- Amines, C20-22-alkyldimethyl
- Details on test material:
- - Physical state: white solid
- Storage: at room temperature in the dark under nitrogen
- Stability: stable
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: activated sludge freshly, sewage treatment plant
- Details on inoculum:
- The substance was tested in duplicate at 16 mg/L. corresponding to 13 mg TOC/L.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 13 mg/L
- Based on:
- other: TOC
Results and discussion
% Degradationopen allclose all
- Value:
- 7
- Sampling time:
- 28 d
- Value:
- 12
- Sampling time:
- 28 d
Any other information on results incl. tables
| Test medium | At the start of the test | On day 28 |
| Blank control (A) | 7.7 - *7.6 | 8.0 |
| Blank control (B) | 7.8 - *7.6 | 7.9 |
| Positive control | 7.7- *7.6 | 8.1 |
| The substance (A) | 7.7- *7.6 | 7.9 |
| The substance (B) | 7.8- *7.6 | 7.9 |
| Toxicity control | 7.7- *7.6 | 8.1 |
* pH adjuested with 1 M HCl
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- The substance was not readily biodegradable under the conditions of the modified Sturm test presently performed.
- Executive summary:
The study procedures describes in this report were based on the OECD Guideline 301B. The study consisted of six bottles, 2 inoculum, 2 test bottles, 1 positive control and 1 toxicity control.Since the substance was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to the 2 -litres test bottles containing medium with microbial organisms and mineral components. The relative biodegradation values calculated from the measurements performed during the test period revealed 7 and 12% biodegradation of the substance, for the duplicate bottles tested. The substance is designated as not readily biodegradable.
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