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Diss Factsheets
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EC number: 204-596-5 | CAS number: 123-05-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: only short notice available
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-ethylhexanal
- EC Number:
- 204-596-5
- EC Name:
- 2-ethylhexanal
- Cas Number:
- 123-05-7
- Molecular formula:
- C8H16O
- IUPAC Name:
- 2-ethylhexanal
- Details on test material:
- - test substance: 2-ethylhexanal, CAS 123-05-7
- purity: > 97 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - analytical monitoring: Gas Liquid Chromatography of samples taken after 0 and 72 hours from freshly prepared media and after 24 and 96 hours from expired media
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- - name of the vehicle: acetone/ tween 80
- preparation of test concentrations: the test substance was dissolved in a 1:99 v/v mixture of acetone/Tween 80 to give an initial stock solution of 500 mg/ml
- serial dilutions of this stock were prepared to give the desired nominal test concentrations; the final solvent concentration in the test was 50 or 100 µl/l of test water
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- - test fish: rainbow trout (Oncorhynchus mykiss)
- mean standard length: 4.9 +/- 0.28 cm
- mean weight: 1.9 +/- 0.27 g
- feeding was stopped 24 h prior to test start
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Details on test conditions:
- - test water: laboratory tap water, dechlorinated by activated carbon and softened by by paasage through an Elga(R) water purification system
- (total hardness: 149 +/- 15 mg/l as CaCO3)
- test vessels: glass aquaria, covered with a glass lid to reduce losses due to the volatile nature of the test compound
- test volume: 50 l
- test concentrations: 0 (control), 0 (solvent control), 1.0, 2.2, 4.6, 10 and 22 mg/l
- No. of fish per concentration: 7
- loading: 0.27 g/l
- photoperiod: 16 h light / 8 h dark
- temperature: 14 +/- 1 °C
- aeration: no
- medium renewal: daily
- test duration: 96 h
- observations: mortality and symptoms of distress after 3, 6, 24, 48, 72, and 96 h
- measurements: pH, temperature, and oxygen content at test start and after 24, 48, 72, and 96 h
- statistics: calculation of LC50 and 95 % confidence limits according to Thompson & Weil (1952)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 8.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
RS-Freetext:
The 96-h LC50 is 5.5 mg/l (geometric mean of 3.7 mg/l [LC0] and 8.2 mg/l [LC100]).
Mortality
---------
Concentration (mg/l) Cumulative mortality (n)
nominal mean measured 3h 6h 24h 48h 72h 96h
0 (control) 0 0 0 0 0 0
0 (solvent control) 0 0 0 0 0 0
1.0 0.81 0 0 0 0 0 0
2.2 1.8 0 0 0 0 0 0
4.6 3.7 0 0 0 0 0 0
10 8.2 0 0 0 7 7 7
22 15 7 7 7 7 7 7
Abnormalities
-------------
In the 3.7 mg/l concentration (mean measured) increased pigmentation was observed already shortly after test start (3 h). After 48 hours, nearly all fish were lying on the bottom and 3 of 7 fish showed loss of equilibrium.
Two of 7 fish were moribund after 24 hours in the 8.2 mg/l concentration. From shortly after test start until found dead, increased pigmentation was seen in nearly all fish and five of 7 fish were lying on the bottom and shoed loss of equilibrium.
The pH was between 7.3 and 7.6 in all test vessels at all
observation times.
The oxygen content was > 7.5 mg/l in all test vessels at all observation times.
In all test vessels the temperature was 15 °C at test start and 14 °C afterwards.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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