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EC number: 201-963-1 | CAS number: 90-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
Additional information
There are no in vivo studies on the absorption of o-anisidine. Oral, dermal and resorption after inhalational exposure can be assumed as systemic effects were observed in toxicological studies. In vitro test showed that metabolism seems to be comparable to other aromatic amines: it encompasses amine group acetylation and ring oxidation. Furthermore activation occurs via O-acetylation as well as peroxidation via enzymes like prostaglandin-H-synthase (note: o-anisidine is also a substrate of thyroid peroxidase, Thyroid peroxidase inhibition with concomitantly decreased thyroid hormone formation is known to induce thyroid tumors. This might be one reason for the increased thyroid tumor incidence observed in male rats in a two year feeding study.). Furthermore, O-demethylation may appear as can be concluded from in vitro studies with microsomes from rat liver.
In urine, the follwing metabolites were found:
N-acetyl-2-methoxyaniline and N-acetyl-4-hydroxy-2-methoxyaniline (in vivo findings). In vitro studies revealed further metabolites e.g. N-(2 -methoxyphenyl)hydroxylamine and o-aminophenol (as well as one structurally unknoown compound).
Subsequently N-(2-Methooxyphenyl)hydroxylamine can be either oxidized to o-nitroanisole or reduced to parental o-anisidin which then can be proceeded to o-aminophenol. The submission substance appears to be a good substrate of a variety of microsomal P450 enzymes (see test with purified cytochromes). P450 2E1 of rabbits seems to be the most efficient enzyme catalyzing the conversion of o-anisidine.
In vivo the highest levels of substance was found in liver, kidneys and in the muscle tissue after 12 h post injection. Over time only a moderate decline in ³H levels was seen in most tissues. Nevertheless, the authors of this study state that this "prolonged ³H retention observed" in most of the examined tissues indicate that "o-anisidine, especially in case of repeated exposure, might accumulate in the body." This is in contrast to the RAR where the authors concluded that due to the log Pow and the rapid excretion o-anisidine is unlikely to bioaccumulate in the organism, this new data indicate that there is a potential for bioaccumulation inherited in o-anisidine.
In several in vivo studies the main excretion route is proved to be urine, about 70 to >90% within 72 to 96 h.The majority was excreted within the first 24 h.
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