4-methylmorpholine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-04-09 - 1991-04-11

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (e.g. non-GLP, no analytical verification of test item concentrations)

Qualifier:

according to guideline

Guideline:

other: EC Directive 79/831/EEC, Annex V, Part C

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): 4-Methylmorpholin
- Substance type: pure active substance
- Physical state: liquid
- Analytical purity: >= 99%
- Lot/batch No.: Tank 8; Prod. no. 2 019 120/1-53
- Stability under test conditions: stable

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution prepared (100 mg/L)
- Differential loading: yes
- Controls: negative control (test medium only)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Waterflea
- Strain: Institut National de Recherche Chimique Appliquée, France
- Source: originally supplied from 'Institut National de Recherche Chimique appliquee, France', and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation (mean and range, SD): 2 to 24 h
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

total hardness: 2.71 mmol/L

Test temperature:

21.3 to 21.5 °C

pH:

7.9 to 8.6

Dissolved oxygen:

7.9 to 8.3 mg/L

Nominal and measured concentrations:

nominal: 0 (control), 12.5, 25, 50, and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20-mL reagent tubes with flat bottom
- Material, size, headspace, fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline (synthetic freshwater prepared on the basis of clean and deionized water with a conductivity of < 0.05 µS/cm)
- Alkalinity: up to pH 4.3: 0.90 ± 0.10 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 674 µSiemens/cm
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h
- Light intensity: diffuse light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- temperature: After 0, 24, and 48 h
- pH values and dissolved oxygen: After 0 and 48 hours
- Mobile daphnids: after 0, 3, 6, 24 and 48 hours


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Mobility:

Concentration (mg/L)	Mobile daphnids after
	3 h	6 h	24 h	48 h
Control	20	20	20	20
12.5	20	20	20	20
25.0	20	20	20	20
50.0	20	20	20	20
100.0	20	20	20	19

pH:

Concentration (mg/L)	0 h	48 h
Control	8.0	7.9
12.5	8.1	8.0
25.0	8.2	8.0
50.0	8.4	8.1
100.0	8.6	8.1

Oxygen content:

- range of O2 (mg/L): 7.9 - 8.3

Temperature:

- range (°C): 21.3 - 21.5

 

Conclusions:

Short-term toxicity of the test substance to invertebrates was investigated by exposing Daphnia magna (waterflea) during 48 hours in a static test, according to the EC directive 79/831/EEC, Annex V, Part C guideline. This resulted in an unbounded EC50 value of > 100 mg/L and a NOEC of 100 mg/L.

Description of key information

Although the key study provided an unbounded EC50 value, a bounded value is used as key value for the chemical safety assessment.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Short-term toxicity of the test substance to invertebrates was investigated by exposing Daphnia magna (waterflea) during 48 hours in a static test, according to the EC directive 79/831/EEC, Annex V, Part C guideline. This resulted in an unbounded EC50 value of > 100 mg/L and a NOEC of 100 mg/L.