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EC number: 231-659-4 | CAS number: 7681-11-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: TThe published literature fulfilled basically scientific principles without GLP compliance statement.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.21 (In Vitro Mammalian Cell Transformation Test)
- GLP compliance:
- no
- Remarks:
- old publication
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- iodide in potassium
- IUPAC Name:
- iodide in potassium
- Details on test material:
- Potassium iodide was purchased from J.T. Baker (Phillipsburg, N.J.).
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Details on mammalian cell type (if applicable):
- The l5178Y mouse-lymphoma cells were originally derived from a methylcholanthrene induced lymphoma (Fischer, 1958) and have subsequently been selected for heterozygous activity at the thymidine kinase locus (Give et al., 1972, 1973, 1975). The l5178Y cell line used in our studies was kindly supplied by Mr. Ken Palmer (Food and Drug Administration, Washington, D.C).
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- other: BALB/c 3T3
- Details on mammalian cell type (if applicable):
- Balb/c 3T3 cells (Dr. D. Brusick, litton Blonetics, Kensington, Md.), derived from done A31 of the Balb/c 3T3 1ine, were used for the transformation studies. The cells were grown in Eagle's Minimal Essential Medium with Eagle's- Salts (EMEM), supplemented with 10% fetal calf serum and 1 % 200 mM glutamine (Gibco). Stock cultures were maintained at 75% confluence. The maintenance medium was EMEM with 5% fetal calf serum.
- Additional strain / cell type characteristics:
- other:
- Metabolic activation:
- without
- Metabolic activation system:
- Iodide is small ion, so the metabolic actication system is unnecessary to the test
- Test concentrations with justification for top dose:
- Mutagenicity test:
100 μg/ml, 500 μg/ml, I mg/ml; 5 mg/ml, 10 mg/ml.
Transformation test:
100 μg/ml, 500 μg/ml, I mg/ml; 5 mg/ml, 10 mg/ml.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- media
- Negative solvent / vehicle controls:
- no
- Remarks:
- no hazard vehicle used
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- Migrated to IUCLID6: In report it was named EMS
- Positive controls:
- yes
- Positive control substance:
- N-dimethylnitrosamine
- Remarks:
- Migrated to IUCLID6: In report it was named DMN
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- Migrated to IUCLID6: In report it was named MNNG
Results and discussion
Test resultsopen allclose all
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: not applicable
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: BALB/c 3T3
- Metabolic activation:
- without
- Genotoxicity:
- other: not applicable
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- negative
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: no data
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1 Mutagenicity of iodide in the L5178Y Test System
Mutagenicity of iodide in the L5178Y Test System | ||
Compound | concentration | .Mutational Frenquency |
Iodide (KI solution) | 10 mg/ml | 1.0 |
5 mg/ml | 0.76 | |
1 mg/ml | 1.33 | |
500μg/ml | 1.67 | |
100 μg/ml | 1.0 | |
Negative control (Media) | 1.0 | |
EMS | 500 μg/ml | 12.0 |
MNNG | 5 μg/ml | 15.0 |
Table 2 Effects of iodide on the Transformation of Balb/c 313 Cells
Effects of iodide on the Transformation of Balb/c 313 Cells | ||
Compound | concentration | .# of foci |
iodide | 10 mg/ml | 0.33 |
5 mg/ml | 2.0 | |
1 mg/ml | 0.61 | |
500μg/ml | 0.61 | |
100 μg/ml | 0.83 | |
Negative control (Media) | 1.22 | |
MNNG | 5 μg/ml | 7.50 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative No effects can be found in either mutagenicity or BALB/c 3T3 cell transformation assays.
Solutions of potassium iodide at concentrations of 0.1–10 mg/ml did not cause mutagenic effects in L5178Y mouse lymphoma cells or transforming activity in Balb/c3T3 cells grown in culture. - Executive summary:
The mutagenic potential to iodide (in potassium iodide ) was studied using the L5178Y mouse (TK+/-) lymphoma assay. The established mutagens ethylmethanesulphonate (EMS) and dimethylnitrosamine (DMN) were highly active in this assay, whereas iodide (KI) was inactive. Using the BALB/c 3T3transformation assayweassessed the transformational capacities of these same agents and the positive mutagen N-ethyl-N-nitro-N-nitrosoguanidine(MNNG). All concentrations of the iodide tested were inactive in this assay. It can be concluded that KI did not possess any biologically significant mutagenic or cell transforming ability.
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