1H-Pyrazole-5-carboxylic acid, 3-bromo-1-(3-chloro-2-pyridinyl)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented GLP & OECD 202 guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Remarks:

The study was conducted according to the guideline in effect at time of study conduct.

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EEC (Method C.2.)

Deviations:

no

Remarks:

The study was conducted according to the guideline in effect at time of study conduct.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

481-150-8

EC Name:

-

Cas Number:

500011-86-9

Molecular formula:

C9H5BrClN3O2

IUPAC Name:

3-bromo-1-(3-chloropyridin-2-yl)-1H-pyrazole-5-carboxylic acid

Constituent 2

Reference substance name:

1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-

IUPAC Name:

1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-

Constituent 3

Reference substance name:

DBC 80

IUPAC Name:

DBC 80

Details on test material:

Sample Code : IN-DBC80
Empirical Formula : C9H5BrClN3O2
Molecular Weight : 302.52
Active Ingredient Content : 96.7% (purity)
Appearance : Tan colour powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Water samples were collected during the definitive experiment after 0 and 48 hours of exposure from the dilution water control, solvent control and the 120 mg/L test solution.

Test solutions

Vehicle:

yes

Details on test solutions:

Stock solution was prepared by dissolving 100 mg of the test substance in 0.08 mL acetone and the volume was made up to 100 mL using distilled water. The appropriate volumes of stock solution were transferred to the exposure medium to obtain the final desired test solution concentrations. The amount of acetone use was such that the final concentration of acetone did not exceed 0.1 mL/L in the test solutions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia magna were reared by IIBAT in 1000 mL bottles. Daphnia magna less than 24 hours old were removed from the culture maintained in thetest room and used for the experiment. During the experiment, daphnids were not fed. Brood daphnids were maintained and cultured in dilution water at the test temperature prior to the start of the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

N/A

Test conditions

Hardness:

The hardness of the dilution water (a 1:1 ratio of hard and soft water) was between 140 and 250 mg/L as CaCO3. The hardness of the control, solvent control, and 120 mg/L test solutions were 194-197 mg/L as CaCO3.

Test temperature:

Temperature ranged from 22.8 to 23.1°Celcius.

pH:

pH ranged from 7.77 to 7.86.

Dissolved oxygen:

Dissolved oxygen concentrations ranged from 7.57 to 7.78 mg/L.

Nominal and measured concentrations:

A nominal concentration of 120 mg/L was chosen for the definitive test based on the results of the preliminary range finding experiment.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Details on results:

No immobility or toxicity signs were observed in the daphnia from any of the test groups after 48 hours of exposure. The 48-hour EC50 was greater than 120 mg/L. The highest concentration tested causing no immobility at the test end was 120 mg/L. The lowest concentration tested causing 100% immobility at the test end was 120 mg/L.

Reported statistics and error estimates:

Statistical analyses were not calculated because there was 100% survival in the limit test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Exposure of Daphnia magna to IN-DBC80 at a concentration of 120 mg/L resulted in no immobilities or sublethal effects.