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EC number: 229-761-9 | CAS number: 6711-48-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, conducted to GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Name of test substance: 3-Diethylaminopropylamine
Test substance No.: 05/0264-1
Charge: 21418856P0
CAS No.: 104-78-9
- Purity: >99.5
- Homogeneity: homogeneous
- Stability: The stability of the test substance under storage conditions over the test period is guaranteed by the sponsor and the sponsor holds this responsibility. - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage and synthetic wastewater.
- Laboratory culture: yes
- Concentration of sludge: 30 mg/L (dry substance) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Initial conc.:
- 32 mg/L
- Based on:
- test mat.
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
915-920 ml dest. water
13 ml inorganic medium
66 ml test substance solution
= Total test volume: 1000 ml
- Aeration of dilution water: yes
TEST SYSTEM
- Method used to create aerobic conditions: aeration
SAMPLING
- Sampling frequency: day 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27, 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
- Abiotic sterile control: yes - Reference substance:
- aniline
- Parameter:
- % degradation (DOC removal)
- Value:
- >= 90 - <= 100
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
= 3 after 10 day(s)
= 20 after 14 day(s)
= 86 after 17 day(s)
Kinetic of control substance (in %):
= 78 after 3 day(s) - Results with reference substance:
- 90-100 % biodegradation after 14 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Data from the related substance 3-aminopropyldiethylamine is used for the read across. The justification for read across is attached to IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- >= 90 - <= 100
- Sampling time:
- 28 d
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-08-10 - 2011-09-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Bis-DMAPA
- Physical state: liquid
- Analytical purity: 97.8%
- Purity test date: 30 May 2011
- Total amine: 16.02 meq/g
- Lot/batch No.: PFW110019
- Supplier: SGS Belgium N.V.
- Stability under test conditions: not reported
- Storage condition of test material: lab - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Totnes Sewage Treatment Works, Totnes, Devon, UK, collected on 9 August 2011
- Storage conditions: aerated at room temperature and pH maintained at 7.0 ± 1.0
- Preparation of inoculum for exposure: 7 days prior to start of exposure the activated sludge was centrifuged, washed and resuspended in the mineral medium and the solids concentration determined
- Initial concentration of sludge in test vessels: 30 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: OECD standard medium
- Test temperature: 22 ± 1 °C
- pH: 7.1-7.2 in the inoculum blank, 4.9-5.1 in the bis-DMAPA bottles
- pH adjusted: yes, to 7.4 prior to testing
- Suspended solids concentration (initial): 30 mg/L
- Continuous darkness: yes, test performed in dark glass bottles
TEST SYSTEM
- Culturing apparatus: Oxitop™ respirometer (Wissenschaftlich-Technische Werkstätten, GmbH, Germany) - each unit consisting of a dark glass 500 mL bottle with Oxitop™ bottle top containing a piezoresistive electronic pressure sensor
- Bottles were situated on a magnetic stirrer in a constant temperature incubator
- Number of culture flasks/concentration: 3 inoculum blanks (inoculum only), 3 positive controls (reference substance (100 mg/L sodium benzoate) + inoculum), 3 test substance bottles (test substance (100 mg/L Bis-DMAPA) + inoculum)
- CO2 produced by respiration was absorbed by a potassium hydroxide (KOH) solution placed in a seal cup in the neck of each bottle
- The Oxitop™ controller collected pressure values from the measuring tops and calculated the BOD (mg/L)
- Oxygen uptake values were corrected for the inoculum blank and biodegradation calculated as % of ThOD
STOCK SOLUTIONS
- A primary stock solution containing 1000 mg/L Bis-DMAPA or 1000 mg/L sodium benzoate was prepared in RO water
- Stock solutions were clear and colourless
- Stock solutions were prepared on day of exposure start
SAMPLING
- Sampling frequency:
Oxygen uptake was recorded automatically every 112 minutes during 28 days.
At day 0 pH was measured in a single bottle of the inoculum blanks and positive controls and was measured and adjusted in all bottles containing the test substance.
At the end of testing pH was measured in all test bottles (day 28).
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes, with sodium benzoate 100 mg/L
DATA ANALYSIS
- Oxygen uptake values were corrected for the inoculum blank and biodegradation calculated as % of ThOD of the substance under consideration. - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- Not applicable.
- Test performance:
- Maximum mean level of biodegradation (based on BOD:ThOD ratio) of sodium benzoate was 63%, > 60% of which was achieved within the 10-day window, thus confirming that the activated sludge was viable.
- Key result
- Parameter:
- other: ThOD
- Value:
- 60
- Sampling time:
- 28 d
- Remarks on result:
- other: 60% degradation was not reached within 10-d window
- Details on results:
- Lag phase days 0 to 6
Degradation phase days 6 to 16
10-day window days 6 to 16
Mean oxygen uptake of inoculum blank was < 60 mg/L, as required in the OECD guideline.
Variation between replicate biodegradation values was < 20% after the 10-day window (at plateau), and on day 28, as required in the OECD guideline.
ThOD of Bis-DMAPA was calculated as 3.42 g O2/g, assuming nitrification. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- This study determined the ready biodegradability of the test substance in a 28-day manometric respirometry test according to OECD guideline 301F in the presence of activated sludge (30 mg SS/L) from a waste water treatment plant treating mainly domestic sewage. The maximum mean level of biodegradation was 60% (expressed as % of the ThOD), showing good potential for biodegradation, however, failing the 10-day window. The results of this test can be considered reliable.
Referenceopen allclose all
Description of key information
Two key studies are available for endpoint coverage. The study of Commander and Stevens (2011) was carried out with the test substance according to the OECD guideline 301F, while the study of BASF (2005) was carried out according to the OECD guideline 301A with a structural analogue substance and used in a read across. Based on the results from both studies, the substance is concluded to be readily biodegradable according to the OECD criteria.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The study of Commander and Stevens (2011) was performed under GLP and assigned a Klimisch score of 1. This study determined the ready biodegradability of the test substance in a 28-day manometric respirometry test according to OECD guideline 301F in the presence of activated sludge (30 mg SS/L) from a waste water treatment plant treating predominantly domestic sewage. The maximum mean level of biodegradation was 60% (expressed as % of the ThOD), showing good potential for biodegradation, however, failing the 10-day window.
Additionally, the ready biodegradability of the substance is further supported by read across to the structural analogue substance 3-aminopropyldiethylamine (CAS 104-78-9). The study of BASF (2005) was carried out according to the OECD guideline 301 under GLP and was assigned a Klimisch score of 1. In this study, a lag phase of 10-14 days was observed. Nevertheless, the substance was considered to be readily biodegradable as the degradation reached 90 to 100% after 20 days and the 10-day window was kept.
Based on the results from both studies, the substance is concluded to be readily biodegradable.
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