Ibuprofen

Toxicity to other aquatic organisms

Administrative data

Endpoint:

toxicity to other aquatic vertebrates

Remarks:

thyroid hormone signaling and endocrine disruptor action in Rana (Lithobates) catesbeiana tadpoles

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

main objective of test set up was the investigation of effects by temperature. Exposure of living tadpoles to ibuprofen, effects (thyroid hormone signaling and endocrine disruptor action) studied in cell cultures

Data source

Reference

Reference Type:

publication

Title:

Influence of temperature on thyroid hormone signaling and endocrine disruptor action in Rana (Lithobates) catesbeiana tadpoles

Author:

S. Austin Hammond, Nik Veldhoen, Caren C. Helbing

Year:

2015

Bibliographic source:

General and Comparative Endocrinology 219: 6–15

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Investigation of the impact of temperature on the TH-induced gene expression programs of premetamorphic Rana (Lithobates) catesbeiana tadpoles following a single injection of 10 pmol/g body wet weight 3,30,5-triiodothyronine (T3). Gene transcript profiling was performed on back skin and tail fin cultured at either 5 or 24 °C following whole animal exposure
at nonpermissive (5 °C) temperature to T3 in the presence and absence of ibuprofen.

GLP compliance:

no

Test material

Test material information

Constituent 1

Specific details on test material used for the study:

no information given on purity

Test organisms

Aquatic vertebrate type (other than fish):

frog

Test organisms (species):

Rana catesbeiana

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

5 to 24 °C

Results and discussion

Details on results:

The transcript profiles of thra, rlk1, dio2, dio3, cirbp, sod, and cat were identical for T3 treated animals with or without added IBUPROFEN suggesting that the temperature effect was the overriding influence on these transcripts. However, T3-specific responses were detected for thibz, thrb, klf9 and cebp1 with the latter three showing indications of perturbation by IBUPROFEN. Both tail fin and back skin demonstrated an approximately 2-fold increase in thibz mRNAs at the nonpermissive temperature that was maintained regardless of the additional IBUPROFEN exposure (p <= 0.002). All cultured tissue samples from animals exposed to T3 ± IBUPROFEN showed augmentation of thibz mRNA levels of 3- to 5-fold-upon shift to the permissive temperature when compared to the low temperature condition. T3 + IBUPROFEN treatment was not different from T3 alone regarding hormone mediated elevation of thrb transcript levels. The T3-induced response at permissive temperature was not affected by IBUPROFEN in the back skin. At the nonpermissive temperature, a _2-fold elevation of klf9 in the back skin from animals coexposed to T3 and IBUPROFEN relative to T3 alone was observed (p <= 0.031).