Dimethyl maleate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VI

Author:

Smyth HF, Carpenter, CP; Weil CS, Pozzani UC, Striegel JA

Year:

1962

Bibliographic source:

Am. Ind. Hyg. Assoc. J. 23, 95-107

Materials and methods

Principles of method if other than guideline:

Concentrated vapour inhalation (no detailed information). consists of subjecting groups of six male and female albino rats to a flowing stream of vapour-ladened air.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl maleate

EC Number:

210-848-5

EC Name:

Dimethyl maleate

Cas Number:

624-48-6

Molecular formula:

C6H8O4

IUPAC Name:

dimethyl (Z)-but-2-enedioate

Details on test material:

- Name of test material (as cited in study report): Melic aicd, dimethyl ester.
- Analytical purity: not reported.

Test animals

Species:

rat

Strain:

other: albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

inhalation: vapour

Details on inhalation exposure:

The vapour-air mixture was generated by passing 2.5 liters/minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 mL of the test chemical contained in a gas-washing bottle. Inhalations were continued for time periods in a logarithmic series with a ratio of two extending from 1.25 to 8 hours, until the inhalation period killing about half the number of rats within 14 days was defined. For inhalation periods of ten, five, and two minutes in duration, a static technique was used whereby 50 to 100 grams of materials, spread over a shallow tray 200 square inches in area, was placed in a 120-liter sealed chamber for at least 24 hours. Six rats were then rapidly introduced by means of a drawer –type cage designed to minimized vapour loss. This static technique, continuing for a maximum if eight hours if necessary, was also employed for mixtures of liquids and for solids. Inhalation of metered vapour concentrations by rats was conducted with flowing streams of vapour prepared by various styles of proportioning pumps.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Remarks on duration:

Inhalation periods are usually of four hours´ duration unless slight toxicity enforces an eight hour period.

Concentrations:

They are in an essentially logarithmic series with a factor of two.

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: no LD50 identified.

Mortality:

The longest inhalation period which permitted all rats to survive the 2-week observation period was 4 hours.