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EC number: 603-562-0 | CAS number: 13223-43-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 10 - 24, 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to GLP in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1200 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 5,7-dimethoxy-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine
- EC Number:
- 603-562-0
- Cas Number:
- 13223-43-3
- Molecular formula:
- C7H9N5O2
- IUPAC Name:
- 5,7-dimethoxy-[1,2,4]triazolo[1,5-a]pyrimidin-2-amine
- Details on test material:
- - Name of test material (as cited in study report): ADTP
- Physical state: off-white solid
- Analytical purity: 99.8%
- Lot/batch No.: 041206-5D
- Expiration date of the lot/batch: n/a
- Stability under test conditions: Test substance was expected to be stable for the duration of testing.
- Storage condition of test material: room temperature
- pH: 7-8 (by wetted pH paper)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Received from Charles River Laboratories, Raleigh, NC on December 28, 2004.
- Age at study initiation: Young adult (9-10 weeks)
- Weight at study initiation: males 198-212 grams and females 126-132 grams at experimental start.
- Fasting period before study: no
- Housing: The animals were singly housed in suspended stainless steel caging with mesh floors which conform to the size recommendations in the most recent Guide for the Care and Use of Laboratory Animals DHEW (NIH). Litter paper was placed beneath the cage and was changed at least three times per week.
- Diet (e.g. ad libitum): Certified Rodent Diet (PMI #5002)
- Water (e.g. ad libitum): Filtered tap water was supplied ad-libitum by an automatic water dispensing system.
- Acclimation period: 13 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-22°C
- Humidity (%): 30-67% RH
- Photoperiod (hrs dark / hrs light): 12-hour light/dark cycle
Administration / exposure
- Type of coverage:
- semiocclusive
- Details on dermal exposure:
- TEST SITE
- Area of exposure: 2-inch x 3-inch
- % coverage: approximately 10% of the body surface
- Type of wrap if used: A 4-ply gauze pad was placed on a dose area. The gauze pad and entire trunk of each animal were then wrapped with 3-inch Durapore tape to avoid dislocation of the pad and to minimize loss of the test substance.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): The pads were removed and the test sites were cleansed of residual test substance.
- Time after start of exposure: After 24 hours of exposure to the test substance
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): Five thousand mg/kg of body weight of the test substance was applied.
- Concentration (if solution): Prior to application, the test substance was moistened with distilled water to achieve a dry paste by preparing a 60% w/w mixture.
- Constant volume or concentration used: yes
- For solids, paste formed: yes
VEHICLE
- Amount(s) applied (volume or weight with unit): In order to ensure adequate contact with the skin, the sample was applied as a dry paste (60% w/w mixture in distilled water). Preliminary solubility testing indicated mixtures in excess of 60% (i.e., 70%-90%) were too dry to ensure adequate skin contact. - Duration of exposure:
- 24 hours
- Doses:
- 5000 mg/kg
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The animals were observed for mortality, signs of gross toxicity, and behavioral changes for several hours after application and at least once daily thereafter for 14 days. Observations included gross evaluation of skin and fur, eyes and mucous membranes, respiratory, circulatory, autonomic and central nervous systems, somatomotor activity and behavior pattern. Particular attention was directed to observation of tremors, convulsions, salivation, diarrhea, and coma.
Individual body weights of the animals were recorded prior to test substance application (initial) and again on Days 7 and 14 (termination).
- Necropsy of survivors performed: yes
- Other examinations performed: All rats were euthanized via CO2 inhalation on Day 14. Gross necropsies were performed on all animals. The external surface of the body and all orifices, tissues, and organs of the thoracic and abdominal cavities were examined. - Statistics:
- no data
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- All animals survived exposure to the test substance.
- Clinical signs:
- other: Apart from reduced fecal volume noted for most animals between Days 1 and 2, there were no other signs of gross toxicity, dermal irritation, adverse clinical signs, or abnormal behavior.
- Gross pathology:
- No gross abnormalities were noted for the animals when necropsied at the conclusion of the 14-day observation period.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the conditions of this study, the single dose acute dermal LD50 of ADTP was greater than 5,000 mg/kg of body weight in male and female rats.
- Executive summary:
An acute dermal toxicity test was conducted in accordance with EPA OPPTS 870.1200 (Acute Dermal Toxicity) with F344 rats to determine the potential for ADTP to produce toxicity from a single topical application. Under the conditions of this study, the single dose acute dermal LD50 of the test substance was greater than 5,000 mg/kg of body weight in male and female rats.
Five thousand milligrams of the test substance per kilogram of body weight was moistened with distilled water and applied to the skin of five male and five female healthy rats for 24 hours. The animals were observed for mortality, signs of gross toxicity, and behavioral changes at least once daily for 14 days. Body weights were recorded prior to application on Day 0, and again on Days 7 and 14 (termination). Necropsies were perfolllled on all animals at sacrifice.
All animals survived exposure to the test substance and gained body weight during the study.
Apart from reduced fecal volume noted for most animals between Days 1 and 2, there were no other signs of gross toxicity, dermal irritation, adverse clinical signs, or abnormal behavior. No gross abnormalities were noted for the animals when necropsied at the conclusion of the 14-day observation period.
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