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Diss Factsheets
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EC number: 700-093-4 | CAS number: 176969-34-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 120 mg/L as nominal concentrations
- Sampling method: Sampling was performed at the start of the exposure (0 h) from the inoculated replicate 7 of each concentration and the control. At the end of the exposure (72 h) samples were taken of the inoculated replicates 1-6 of the test concentrations and of the inoculated replicates 1-6 of the control group. The replicates were combined before sampling. Additionally, samples were collected from the uninoculated replicate 0 of each test concentration and the control at the end of the exposure (72 h).
- Sample storage conditions before analysis: Samples were transported to the Analytical Laboratory on the day of sampling. Ambient temperature
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock test solution (133.2 mg/L) was prepared by weighing 266.36 mg test substance and diluting it in 2 L test water. The mixture was stirred vigorously for about 10 minutes on a magnetic stir plate. To reach the correct nominal concentration (120 mg/L) at test start, 10 mL of inoculum culture was added to 90 mL of the stock test solution. The pH value of the stock solution was initially 4.3 and was adjusted with 1 molar NaOH to pH 8.4 prior to addition of algal inoculum.
The stock solution was visibly clear and slightly pink following preparation.
- Controls: The analytically determined concentrations of the test substance in the test solutions during the exposure period were within the range of + 20% of the nominal concentrations.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: KORSHIKOV SAG 61.81
- Source (laboratory, culture collection): The algae were obtained at regular intervals from SAG (Collection of algal cultures in Göttingen) and were kept in liquid culture in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology, BASF SE, Ludwigshafen, Germany.
- Age (on study day 0): A stock algal culture is maintained continuously at the test facility. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 3 days at 21 – 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test (study day 0).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.5 – 22.7°C
- pH:
- 7.8-8.6
- Nominal and measured concentrations:
- 0 (control), 120 mg/L as nominal concentrations
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): closed, plugged with gas permeable silicone sponge caps
- Material, size, headspace, fill volume: glas, nominal volume 250 mL, fill volume: 100 mL
- Initial cells density: 5000 cells/mL
- No. of vessels per concentration (replicates): 6 replicates for each test substance concentration
- No. of vessels per control (replicates): 6 replicates for the Control
- 1 additional replicate per test group for initial concentration control analysis only.
- 1 additional uninoculated (without algae) replicate per test group for background fluorescence correction and to determine the influence of the test design on test substance stability.
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test medium was prepared according to OECD Guideline for Testing of Chemicals, No. 201 Algal growth inhibition test, Mar 2006. The pH value of the test medium was 8.1.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: 7.8-8.6
- Light intensity and quality: Average 4900 lux (within ± 15% variability) at a wave length of 400 - 700 nm
- Shaking rate: Continuous (approx. 85 rpm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] Test chamber: Vötsch Industrietechnik GmbH Bioline (VB1014) controlled climate cabinet.
- Chlorophyll measurement: Algal growth measured as in vivo chlorophyll-a fluorescence (pulsed excitation with light flashes having a wavelength of 432 nm)
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0 (control), 120 mg/L as nominal concentrations - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: algal yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: algal yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: algal yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- The cell multiplication factor in the untreated control was 198-fold after 72 hours. (counted cell density: 99 0000 cells/mL)
- Observation of abnormalities (for algal test): no remarkable observations - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: The ErC50 (72 h) of the control substance potassium dichromate was 0.97 mg/L (Date of the last control experiment: 29 Mar 2010 , project number: 60E0063/043031). These results indicate that the algae are responding normally to toxicant stress.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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