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EC number: 273-870-4 | CAS number: 69103-20-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study performed under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- inspected August 2005; signature: November 2005
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2-dimethyl-3-(3-methylpenta-2,4-dienyl)oxirane
- EC Number:
- 273-870-4
- EC Name:
- 2,2-dimethyl-3-(3-methylpenta-2,4-dienyl)oxirane
- Cas Number:
- 69103-20-4
- Molecular formula:
- C10H16O
- IUPAC Name:
- 2,2-dimethyl-3-(3-methylpenta-2,4-dienyl)oxirane
- Test material form:
- gas under pressure: refrigerated liquefied gas
- Details on test material:
- - Physical state: Liquid
- Storage condition of test material: Approximately 4°C, in the dark, under nitrogen.
- Other: Pale yelow
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S9
- Test concentrations with justification for top dose:
- Preliminary test (toxicity test): 0, 0.15, 0.5, 1.5, 5, 15, 50, 150, 500, 1500 and 5000 ug/plate.
Range-finding test:
Salmonella strains: 15, 50, 150, 500, 1500, 5000 ug/plate.
E.coli strain WP2uvrA·: 50, 150, 500, 1500, 5000 ug/plate.
Main test: 50, 150, 5000, 1500 and 5000 ug/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: acetone
- Justification for choice of solvent/vehicle: The test material was immiscible in sterile distilled water and dimethyl sulphoxide at 50 mg/ml
but was fully miscible in acetone at the same concentration in solubility checks performed.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- N-ethyl-N-nitro-N-nitrosoguanidine
- benzo(a)pyrene
- other: 2-Aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation)
DURATION
- Exposure duration: All of the plates were incubated at 37ºC for approximately 48 hours and the frequency of revertant colonies assessed using a Domino colony counter.
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- The test material may be considered positive in this test system if the following criteria are met:
The test material should have induced a reproducible, dose-related and statistically significant increase in the revertant count in at least one or more bacterial strain with or without metabolic activation. - Statistics:
- Statistical methods, as recommended by the UKEMS Subcommittee on Guidelines for Mutagenicity Testing, Report - Part III (1989).
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- TA100 at 5000 ug/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1. Range finding test
Range-finding test with and without S9 | |||||||||||
Number of revertants (mean number of colonies per plate) | |||||||||||
S9 Mix | Test substance concentration (ug/plate) | TA100 | Mean | TA1535 | Mean | WP2uvrA- | Mean | TA98 | Mean | TA1537 | Mean |
- | 0 | 74 | 89 | 22 | 27 | 24 | 21 | 24 | 20 | 11 | 10 |
90 | 32 | 16 | 17 | 7 | |||||||
102 | 26 | 22 | 19 | 11 | |||||||
- | 50 | 95 | 97 | 29 | 32 | 17 | 17 | 16 | 17 | 8 | 8 |
90 | 37 | 19 | 16 | 7 | |||||||
105 | 30 | 15 | 19 | 9 | |||||||
- | 150 | 94 | 95 | 21 | 24 | 12 | 14 | 15 | 23 | 8 | 10 |
C | 25 | 13 | 15 | 8 | |||||||
95 | 15 | 17 | 38 | 15 | |||||||
- | 500 | 118 | 105 | 24 | 29 | 20 | 23 | 25 | 23 | 7 | 9 |
87 | 34 | 21 | 22 | 15 | |||||||
110 | 30 | 29 | 22 | 6 | |||||||
- | 1500 | 89 | 95 | 28 | 26 | 20 | 21 | 18 | 17 | 4 | 7 |
106 | 26 | 24 | 12 | 12 | |||||||
89 | 24 | 18 | 21 | 6 | |||||||
- | 5000 | 97 | 106 | 33 | 34 | 20 | 20 | 19 | 12 | 5 | 6 |
111 | 30 | 18 | 7 | 10 | |||||||
109 | 39 | 22 | 11 | 4 | |||||||
+ | 0 | 85 | 89 | 13 | 21 | 21 | 21 | 24 | 24 | 16 | 11 |
93 | 11 | 25 | 25 | 10 | |||||||
89 | 10 | 16 | 23 | 6 | |||||||
+ | 50 | 88 | 81 | 12 | 24 | 20 | 24 | 29 | 24 | 6 | 12 |
74 | 12 | 23 | 30 | 13 | |||||||
82 | 9 | 29 | 12 | 17 | |||||||
+ | 150 | 85 | 91 | 11 | 15 | 15 | 15 | 25 | 26 | 15 | 20 |
89 | 6 | 17 | 30 | 19 | |||||||
98 | 7 | 13 | 23 | 26 | |||||||
+ | 500 | 72 | 78 | 10 | 21 | 21 | 21 | 22 | 24 | 10 | 14 |
79 | 10 | 21 | 23 | 15 | |||||||
83 | 3 | 20 | 26 | 18 | |||||||
+ | 1500 | 94 | 79 | 1 | 21 | 21 | 21 | 16 | 19 | 19 | 14 |
76 | 13 | 22 | 16 | 11 | |||||||
67 | 10 | 20 | 24 | 13 | |||||||
+ | 5000 | 78 | 79 | 7 | 23 | 22 | 23 | 24 | 15 | 15 | 15 |
77 | 11 | 27 | 9 | 17 | |||||||
81 | 7 | 20 | 13 | 13 | |||||||
Positive controls | ENNG | ENNG | ENNG | 4NQO | 9AA | ||||||
Concentration (ug/plate) | 3 | 5 | 2 | 0.2 | 80 | ||||||
without S9 | 436 | 469 | 193 | 191 | 828 | 858 | 120 | 128 | 1034 | 869 | |
487 | 202 | 894 | 146 | 1160 | |||||||
484 | 179 | 851 | 118 | 412 | |||||||
Positive controls | 2AA | 2AA | 2AA | BP | 2AA | ||||||
Concentration (ug/plate) | 1 | 2 | 10 | 5 | 2 | ||||||
With S9 | 767 | 834 | 187 | 221 | 314 | 282 | 166 | 214 | 222 | 250 | |
845 | 245 | 277 | 223 | 273 | |||||||
891 | 232 | 252 | 254 | 254 |
Table 2. Main test
Main test with and without S9 | |||||||||||
Number of revertants (mean number of colonies per plate) | |||||||||||
S9 Mix | Test substance concentration (ug/plate) | TA100 | Mean | TA1535 | Mean | WP2uvrA- | Mean | TA98 | Mean | TA1537 | Mean |
- | 0 | 84 | 94 | 28 | 23 | 20 | 21 | 17 | 18 | 10 | 9 |
88 | 17 | 18 | 18 | 9 | |||||||
111 | 25 | 24 | 19 | 9 | |||||||
- | 50 | 94 | 93 | 29 | 22 | 20 | 22 | 21 | 20 | 8 | 12 |
86 | 22 | 20 | 16 | 17 | |||||||
98 | 29 | 26 | 24 | 10 | |||||||
- | 150 | 109 | 107 | 30 | 21 | 17 | 21 | 17 | 14 | 6 | 8 |
116 | 32 | 24 | 15 | 8 | |||||||
97 | 29 | 33 | 9 | 9 | |||||||
- | 500 | 112 | 109 | 32 | 18 | 16 | 18 | 19 | 20 | 10 | 8 |
103 | 30 | 20 | 21 | 4 | |||||||
113 | 22 | 19 | 21 | 10 | |||||||
- | 1500 | 96 | 98 | 30 | 18 | 11 | 18 | 18 | 20 | 11 | 11 |
102 | 39 | 17 | 30 | 15 | |||||||
96 | 19 | 25 | 11 | 8 | |||||||
- | 5000 | 113 | 110 | 31 | 13 | 5 | 13 | 6 | 11 | 5 | 6 |
100 | 28 | 23 | 8 | 6 | |||||||
118 | 32 | 11 | 18 | 6 | |||||||
+ | 0 | 103 | 98 | 10 | 10 | 36 | 30 | 21 | 27 | 15 | 19 |
96 | 10 | 26 | 29 | 20 | |||||||
96 | 11 | 27 | 31 | 21 | |||||||
+ | 50 | 104 | 103 | 10 | 13 | 24 | 27 | 23 | 24 | 13 | 18 |
101 | 19 | 22 | 26 | 24 | |||||||
105 | 11 | 35 | 24 | 16 | |||||||
+ | 150 | 106 | 104 | 11 | 9 | 19 | 21 | 27 | 25 | 11 | 14 |
105 | 9 | 24 | 18 | 19 | |||||||
100 | 7 | 19 | 29 | 13 | |||||||
+ | 500 | 101 | 97 | 15 | 13 | 26 | 21 | 23 | 22 | 12 | 13 |
102 | 9 | 24 | 22 | 13 | |||||||
89 | 16 | 12 | 22 | 13 | |||||||
+ | 1500 | 82 | 93 | 12 | 13 | 19 | 23 | 28 | 23 | 11 | 18 |
115 | 17 | 24 | 21 | 27 | |||||||
83 | 11 | 26 | 19 | 15 | |||||||
+ | 5000 | 104 | 93 | 9 | 14 | 25 | 25 | 20 | 20 | 16 | 16 |
101 | 18 | 25 | 21 | 12 | |||||||
73 | C | 26 | 19 | 19 | |||||||
Positive controls | ENNG | ENNG | ENNG | 4NQO | 9AA | ||||||
Concentration (ug/plate) | 3 | 5 | 2 | 0.2 | 80 | ||||||
without S9 | 493 | 493 | 307 | 302 | 600 | 600 | 161 | 153 | 1145 | 1179 | |
497 | 323 | 613 | 133 | 1131 | |||||||
488 | 277 | 588 | 165 | 1262 | |||||||
Positive controls | 2AA | 2AA | 2AA | BP | 2AA | ||||||
Concentration (ug/plate) | 1 | 2 | 10 | 5 | 2 | ||||||
With S9 | 482 | 706 | 266 | 249 | 310 | 299 | 264 | 249 | 119 | 189 | |
807 | 263 | 309 | 237 | 117 | |||||||
828 | 218 | 278 | 245 | 332 |
BP - Benzo(a)pyrene
2AA - 2-Aminoanthracene
ENNG - N-ethyl-N'-nitro-N-nitrosoguanidine
4NQO - 4 -Nitroquinoline-1-oxide
9AA - 9 -Aminoacridine
C - Contaminated
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the conditions of this study the test material was considered to be non-mutagenic in the presence and absence of S9 activation. - Executive summary:
The study was performed to the requirements of OECD Guideline 471, EU Method B13/14 and Japanese guidelines for bacterial mutagenicity testing under GLP, to evaluate the potential mutagenicity of the test substance in a bacterial reverse mutation assay using S.typhimurium strains TA98, TA100, TA1535, TA1537 and E.coli strain WP2uvrA- in both the presence and absence of S-9 mix. A preliminary test was performed to determine the toxicity of the test material. A range-finding study was performed to determine the doses used for the main test. In the main test, the plate incorporation method was used and was evaluated at a concentration of up to 5000 µg/plate. Positive controls appropriate for each strain, in the presence and absence of S9 -mix, were included. The test substance did not induce any significant, reproducible increases in the observed number of revertant colonies in any of the strains tested, either in the presence or absence of S9-mix. The vehicle (acetone) control plates gave counts of revertant colonies within the normal range. All of the positive control chemicals used in the test induced marked increases in the frequency of revertant colonies, both with or without metabolic activation. Thus, the sensitivity of the assay and the efficacy of the S9-mix were validated. The test material caused a visible reduction in the growth of the bacterial background lawns at 5000 µg/plate to the majority of the Salmonella strains in the absence of S9 and to TA100 and TA1535 in the presence of S9. No toxicity was noted for Escherichia coli strain WP2uvrA-. The test material was, therefore, tested up to the maximum recommended dose level of 5000 µg/plate. No test material precipitate was observed on the plates at any of the doses tested in either the presence or absence of S9-mix. It was concluded that, under the conditions of this assay, the test substance gave a negative, i.e. non-mutagenic response in S.typhimurium strains TA98, TA100, TA1535, TA1537 and E.coli strain WP2uvrA- in the presence and absence of S-9 mix.
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