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EC number: 208-796-3 | CAS number: 542-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-10-31 till 1989-11-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Reliable without restrictions; Guideline study (OECD 404; Irritant effects on rabbit skin) according to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,4-Diamino-6-methyl-1,3,5-triazine
- IUPAC Name:
- 2,4-Diamino-6-methyl-1,3,5-triazine
- Reference substance name:
- 6-methyl-1,3,5-triazine-2,4-diyldiamine
- EC Number:
- 208-796-3
- EC Name:
- 6-methyl-1,3,5-triazine-2,4-diyldiamine
- Cas Number:
- 542-02-9
- Molecular formula:
- C4H7N5
- IUPAC Name:
- 6-methyl-1,3,5-triazine-2,4-diamine
- Details on test material:
- - Name of test material (as cited in study report): Acetoguanamine
- Physical state: solid (white powder)
- Analytical purity: 99.2 %
- Lot/batch No.: 10/08/89 AG
Constituent 1
Constituent 2
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Froxfield Farms (U.K.) Ltd., Petersfield, Hampshire, England
- Age at study initiation: approx. 12 weeks
- Weight at study initiation: 2.5 - 2.7 kg
- Housing: idividually in metal cages with perforated floors.
- Diet (e.g. ad libitum): SDS Standard Rabbit Diet (ad libitum)
- Water (e.g. ad libitum): tap water (ad libitum)
- Acclimation period: "the rabbit selected were all acclimated to the laboratory environment", but no data on time period.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): approx. 19 °C
- Humidity (%): 30-70 %
- Air changes (per hr): approx. 19 per hour
- Photoperiod (hrs dark / hrs light): lighting was controlled by means of a time switch to give 12 hours of artificial light (0700-1900 hours) in each 24 hours period.
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- water
- Controls:
- no
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 g Acetoguanamine
VEHICLE
- Amount(s) applied (volume or weight with unit): 0.5 mL distilled water
- Lot/batch no. (if required): 10/08/89 AG
- Purity: 99.2 % - Duration of treatment / exposure:
- four hours
- Observation period:
- Examintation of the treated skin was made on Day 1 (i.e. approximately 30 minutes after removal of the patches) and on Days 2, 3 and 4.
- Number of animals:
- three
- Details on study design:
- TEST SITE
- Area of exposure: dorso-lumbar region
- Type of wrap if used: 2.5 cm square gauze pad
REMOVAL OF TEST SUBSTANCE
- Washing (if done): treatment site was washed using water to remove any residual test substance
- Time after start of exposure: four hours
SCORING SYSTEM:
Erythema and eschar formation:
0: No erythema
1: Very slight erythema (barely perceptible)
2: Well-defined erythema
3: Moderate to severe erytehma
4: Severe erythema (beet redness) to slight eschar formation(injuries in depth)
Oedema formation:
0: No oedema
1: Very slight oedema (barely perceptible)
2: Slight oedema (edges of area well-defined by definite raising)
3: Moderate oedema (raised approximately 1 millimetre)
4: Severe oedema (raised more than 1 millimetre and extending beyond the area of exposure)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- other: 30 minutes after removal of the patches and on days 2, 3 and 4
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- other: 30 minutes after removal of the patches and on days 2, 3 and 4
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- other: 30 minutes after removal of the patches and on days 2, 3 and 4
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- other: 30 minutes after removal of the patches and on days 2, 3 and 4
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- other: 30 minutes after removal of the patches and on days 2, 3 and 4
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- other: 30 minutes after removal of the patches and on days 2, 3 and 4
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritant / corrosive response data:
- The treated skin of all three animals was scored for erythema and oedema. The test substance caused no adverse effects to the skin.
- Other effects:
- None of the animals showed any response to treatment throughout the observation period.
Any other information on results incl. tables
Table 1: Dermal reactions elicited by Acetoguanamine.
Rabbit Number and Sex |
Bodyweight (Day 1) |
E = Erythema O = Oedema |
Day |
|||
1* |
2 |
3 |
4 |
|||
803 (female) |
2525 g |
E O |
0 0 |
0 0 |
0 0 |
0 0 |
804(female) |
2650 g |
E O |
0 0 |
0 0 |
0 0 |
0 0 |
805(female) |
2670 g |
E O |
0 0 |
0 0 |
0 0 |
0 0 |
* Approximately 30 minutes after removal of the dressing
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: other: EEC Directive 83/467/EEC Annex VI Part II
- Conclusions:
- In a dermal irritation study according to OECD 404 three New Zealand White strain rabbits were semi-occlusive dermally exposed to 0.5 g Acetoguanamine for four hours. Examination of the treated skin was made on Day 1 (i.e. approx. 30 minutes after removal of the test substance) and on Day 2, 3 and 4. None of the animals showed any response to treatment throughout the observation period.
Therefore no classification for skin irritation is required for Acetoguanamine. - Executive summary:
In a dermal irritation study according to OECD 404 three New Zealand White strain rabbits were semi-occlusive dermally exposed to 0.5 g of Acetoguanamine for four hours. Examination of the treated skin was made on Day 1 (i.e. approx. 30 minutes after removal of the test substance) and on Day 2, 3 and 4. None of the animals showed any response to treatment throughout the observation period. Therefore no classification for skin irritation is required for Acetoguanamine.
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