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EC number: 203-253-7 | CAS number: 104-93-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
Test material
- Reference substance name:
- 4-methylanisole
- EC Number:
- 203-253-7
- EC Name:
- 4-methylanisole
- Cas Number:
- 104-93-8
- Molecular formula:
- C8H10O
- IUPAC Name:
- 1-methoxy-4-methylbenzene
- Details on test material:
- - Name of test material (as cited in study report): p-Methylanisol
- Physical state: liquid - colourless-yellowish
- Analytical purity: > 99.5 %
- Lot/batch No.: P 16
- Stability under test conditions: stability was ensured for at least the study period
- Storage condition of test material: at room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, Germany
- Age at study initiation: approx. 8-9 weeks
- Average weight at study initiation: males 272 +/- 9.6 g; females 192 +/- 4.5 g
- Housing: in groups of 5 animals in cages type D III of Becker without bedding
- Diet: KLIBA rat/mouse laboratory diet 24-343-4 10 mm pellets, Klingentalmühle AG, Kaiseraugst, Switzerland, ad libitum during post exposure observation period
- Water: ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature: 20 - 24 °C
- Humidity: 30 - 70 %
- Air changes per hr: fully air-conditioned rooms
- Photoperiod (hrs dark/hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- Exposure system
Whole-body inhalation system : IKA 02 (glass-steel construction ; BASF Aktiengesellschaft, volume ca. 200 l). The animals were kept singly in compartmentalized wire cages, and were exposed in the chamber.
Generation of the inhalation atmosphere
The test substance was dosed unchanged. A vapor air mixture was generated by means of a
- continuous infusion pump UNITA I (B . Braun)
- glass vaporizer with thermostat (BASF)
By means of the continuous infusion pump the test substance was supplied to the heated vaporizer. The vapors that developed were mixed with supply air and passed into the inhalation system.
Exposure
The following air flow/s (supply air) was set:
blast air : 3000 l/h
The supply air was conditioned via a central air-conditioning system. The exposure system was placed in an air-conditioned laboratory. Temperatures in the exposure system were 19-25°C. Deviations from this specification which would have had any adverse effect on the results of the study did not occur. The inhalation atmosphere was offered to the animals for inhalation for 4 hours. - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Gas chromatographical method.
- Duration of exposure:
- 4 h
- Concentrations:
- 6.1 mg/l (maximum technically achievable concentration)
- No. of animals per sex per dose:
- 5 animals per sex per dose
- Control animals:
- no
- Details on study design:
- Mean concentration of four samples: 6.10 +/- 0.24 mg/l
Control group: historical (air)
Vehicle: air
Air flow: 3000 l/h
Generation of the inhalation atmosphere: via a continuous infusion pump and a heated vaporizer; developed vapors were mixed with supply air; tested was the maximum concentration that could be achieved technically
Observation period: 14 d
Observations:
Body weight: weekly (before the beginning, day 7, day 14)
Clinical findings: at least once/work day
Mortality: daily
Pathology: gross necropsy - Statistics:
- Concentration/effect relationship: Binomial test following Wittig H: Mathematische Statistik 1974, pp. 32-35
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC0
- Effect level:
- 6.1 mg/L air
- Exp. duration:
- 4 h
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 6.1 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- No mortality occured during the study.
- Clinical signs:
- other: Clinical signs during exposure: Accelerated respiration (mainly within the first hour, then turning into irregular or intermittent respiration) and eyelid closure directly after the beginning of the exposure; salivation, abdominal position, squatting pos
- Body weight:
- The body weight gain was retarded in the second week of the observation period in comparison to the historical controls.
- Gross pathology:
- No pathological findings were noted.
Any other information on results incl. tables
Table 1: Mean body weight of the treated animals compared to historical controls
Mean body weight (g) |
before the study |
after 7 days |
after 14 days |
|||
|
male |
female |
male |
female |
male |
female |
test group |
272 |
192 |
300 |
213 |
310 |
220 |
historical (air) control |
248 |
177 |
285 |
196 |
318 |
211 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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