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EC number: 259-766-1 | CAS number: 55699-10-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study in compliance with GLP-criteria.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-tert-butyl-3-hydroxy-2,6-xylylacetonitrile
- EC Number:
- 259-766-1
- EC Name:
- 4-tert-butyl-3-hydroxy-2,6-xylylacetonitrile
- Cas Number:
- 55699-10-0
- Molecular formula:
- C14H19NO
- IUPAC Name:
- 2-(4-tert-butyl-3-hydroxy-2,6-dimethylphenyl)acetonitrile
- Details on test material:
- - Name of test material (as cited in study report): 4-tert-Butyl-3-hydroxy-2,6-xylylacetonitrile
- Physical state/ Appearance: Solid / white to yellowish
- Homogeneity: Homogeneous
- Storage conditions: Storage at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- TEST SUBSTANCE:
The test substance was weighed in the required amounts for the test concentrations directly to the test vessels. Then 180 mL demineralized water were added. The mixtures were stirred for 20 ± 2 hours at room temperature.
REFERENCE SUBSTANCE:
0.5 g of the reference substance was added to about 800 mL of demineralized water and stirred at room temperature until the reference substance was completely dissolved. The pH value of the stock solution was measured and adjusted to 7.4 with 1 M sodium hydroxide solution. Following this the stock solution was made up to 1L with demineralized water. The stock solution appeared colourless-clear.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant of Mannheim/Baden Württemberg, Germany was collected on 19 September 2007 from the aeration tank of the plant. The activated sludge suspension was washed 3 times with tap water and sieved with a fine woven mesh (mesh size about 1 mm). This suspension was adjusted to a concentration of 7.5 g/L dry weight and pre-aerated over night. After the pre-aeration the lost water of the suspension was replaced. An aliquot of this suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- ambient (20±2 °C)
- Dissolved oxygen:
- - Oxygen concentration during aeration: > 2.5 mg/L
- Oxygen concentration immediately before measurement: > 6.5 mg/L - Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 62.5, 125, 250, 500 and 1000 mg/L.
- Details on test conditions:
- EXPERIMENTAL PROCEDURE:
8 mL of the synthetic medium were added to the test vessels with test substance preparations, which were prepared the previous day. Aliquots of the stock solution with reference substance were pipetted to the test vessels and made up with demineralized water to a volume 180 mL. After that 8 mL synthetic medium were pipetted in each test vessel with reference substance. To prepare the blank control assays 180 mL of demineralized water and 8 mL synthetic medium were mixed. The pH-values were measured in all test vessels. An adjustment was not necessary. 12 mL demineralized water were added to each test vessel. After addition of 50 mL of the inoculum suspension (dry weight 7.5 g/L) the incubation was started by aeration of the test vessels with pressure air. The vessels for the blank control assays were prepared according to the same procedure without addition of test- or reference substance. After 180 minutes incubation the mixtures in the test vessels were placed subsequently into a closed oxygen measuring cell. A chart recorder recorded the oxygen respiration rates. The oxygen consumption of the blank control BC1 was measured first. The oxygen consumption of the reference substance assays (RS) was measured in the sequence RS1, RS2 and RS3. Subsequently the oxygen consumption of the test substance assays was measured in the sequence TS1, TS2, TS3, TS4 and TS5. The oxygen consumption of the blank control BC2 was measured at last.
TEST PARAMETERS:
- Incubation time: 180 minutes
- Test vessels: Erlenmeyer-vessel (nominal volume 250 mL)
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
- Duration of the measurement of oxygen consumption: 8 - 10 min
- Sludge concentration in the test vessel: 1.5 g/L
- Number of test assays per concentration: 1 - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- EC10
- Effect conc.:
- 490 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC50 = 6 mg/L
- Reported statistics and error estimates:
- The values were given with an accuracy of 2 significant digits. The degree of inhibition was evaluated by Probit analysis according to Finney.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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