Pentasodium 4-amino-6-[[5-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-2-sulphonatophenyl]azo]-3-[(2,5-disulphonatophenyl)azo]-5-hydroxynaphthalene-2,7-disulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Principles of method if other than guideline:

Guidelines followed

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

The concentration of the test substance at the beginning and at the end of the test was analysed. All test medium samples for the analytical determinations were taken after mixing of the test solutions.

Vehicle:

no

Details on test solutions:

Test concentrations: Based on a pretest, the test concentrations were chosen as 58, 100 and 180 mg/L nominal.

Test organisms (species):

Daphnia magna

Details on test organisms:

The daphnids were bred in the laboratories of the Textile Dyes Division, Product Ecology TF under standardized conditions. For the test, animals with age of less than 24 h were used. The quality of the daphnids will be checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8-1.5 mg/L). For each concentration, 20 Daphnia magna were used.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

None

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

21.2 °C

pH:

8.0

Dissolved oxygen:

97-98 %

Salinity:

No data

Nominal and measured concentrations:

Nominal: 0, 58, 100 and 180 mg/L
Measured: 0, 63, 107.9 and 193.7 mg/L

Details on test conditions:

Experimental conditions: 48 h at 21 °C, 16 h light/8 h dark
The daphnids were exposed to the test substance in 250 mL beakers containing 100 - 200 mL test medium with 10 animals per beaker. All tests are running in duplicate. During exposure the animals were kept at 21±1°C with 16 h illumination.

Reference substance (positive control):

yes

Remarks:

(potassium dichromate)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

183.1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

63 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

The reported results are related to the analytical concentrations of the test substance. During test duration the test substance concentrations in the analysed test media were in the range of 106.3 - 109.3 % of the nominal values. Therefore under the test conditions the test substance was sufficiently stable. However, in all test concentrations a sedimentation of the test substance after 48 h was observed. In the control no immobilization of the daphnids was observed after 48 h. Similarly no immolization was observed at 58 mg/L test concentration, while 10 and 50% immobilization was observed at 100 and 180 mg/L concentrations respectively. Based on these findings, the EC50 value was calculated to be 183.1 mg/L. The EC100 value was clearly higher than 193.7 mg/L.

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Analytical results of the test substance:

Concentration start			Concentration end
Nominal concentration mg/L	mg/L	% of nominal	mg/L	% of nominal	Average concentration mg/L
58	62.5	107.8	63.4	109.3	63.0
100	106.5	106.5	109.3	109.3	107.9
180	191.4	106.3	196.0	108.9	193.7

Validity criteria fulfilled:

yes

Conclusions:

The 48 h EC50 of the test substance to Daphnia magna for immobilisation was 183.1 mg/L (measured concentration).

Executive summary:

The acute toxicity of the test substance (of 100 % purity) to Daphnia magna was determined in a 48-h static test according to OECD Guideline 202 and EU Method C.2.

Daphnia magna were exposed to nominal concentrations of the test substance (0, 58, 100 and 180 mg/L) for 48-h in static test conditions at 21 °C. During test duration the test substance concentrations in the analyzed test media were in the range of 106.3 - 109.3 % of the nominal values. Therefore, under test conditions the test substance was sufficiently stable.

In the control no immobilization of the daphnids was observed after 48 h. Similarly, no immobilization was observed at 58 mg/L test concentration, while 10 and 50 % immobilization was observed at 100 and 180 mg/L concentrations respectively. Based on these findings, the EC50 value was calculated to be 183.1 mg/L. The EC100 value was clearly higher than 193.7 mg/L.

Description of key information

FAT 40171/D  had no acute toxic effects on Daphnia magna with a 48-hour EC50 of 183.1 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

183.1 mg/L

Additional information

The acute toxicity of Reactive Black 039 (of 100 % purity) to Daphnia magna was determined in a 48 h static test according to OECD Guideline 202 and EU Method C.2.

Daphnia magna were exposed to nominal concentrations of the test substance (0, 58, 100 and 180 mg/L) for 48 h in static test conditions at 21 °C. During test duration the test substance concentrations in the analyzed test media were in the range of 106.3 - 109.3 % of the nominal values. Therefore, under test conditions the test substance was sufficiently stable.

In the control no immobilization of the daphnids was observed after 48 h. Similarly no immolization was observed at 58 mg/L test concentration, while 10 and 50 % immobilization was observed at 100 and 180 mg/L concentrations respectively. Based on these findings, the EC50 value was calculated to be 183.1 mg/L. The EC100 value was clearly higher than 193.7 mg/L.