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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Study period:
2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Principles of method if other than guideline:
Method is in most parts equivalent to the OECD TG 202 Daphnia sp., Acute immobilisation test and reproductiontest, Part I - The 24h EC50 Acute immobilisation test.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chlorobenzothiazole
EC Number:
210-415-0
EC Name:
2-chlorobenzothiazole
Cas Number:
615-20-3
Molecular formula:
C7H4ClNS
IUPAC Name:
2-chloro-1,3-benzothiazole

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Stock solution:
-Aliquot of the substance (49.9 mg/L) dissolved in water (1 L)
- Mixture treated for 60 s at 8000 rpm with an ultra-turrax
- stirred by magnetic stirrer for 24 h
- filtered

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
neonates

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
Dilution water was reconstituted deionized tap water with a total hardness of 14.7 °dH (German hardiness degrees) measured at
test start
Test temperature:
18-22 °C
pH:
neutral (not reported). pH and oxygen concentration measured at the end of the test
Dissolved oxygen:
not reported but pH and oxygen concentration measured at the end of the test. As no deviation from the guideline is reported, sufficient oxygen saturation assumed for all vessels and tests
Nominal and measured concentrations:
Measured. Stability of the test concentration during exposure was verified by chemical analysis with HPLC at 0 and 48 hours. Recovery was 98.8 - 101.1 % of nominal values at start of incubation (0 h), and 98.0 - 100.5 % of nominal values at end of incubation (48 h)
Details on test conditions:
- 50 ml glass vessels holding 10 neonates in 20 ml of test medium
- 2 replicates per concentration and for control

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour and locomotory actions
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
8.32 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour and locomotory actions
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 10 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour and locomotory actions
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
2.5 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour and locomotory actions
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.17 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour and locomotory actions
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
10 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour and locomotory actions
Reported statistics and error estimates:
Recovery of test substance in incubation media was 98.8 - 101.1 % of nominal values at start of incubation (0 h), and 98.0 - 100.5 % of nominal values at the end of incubation (48 h)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50 of 2-chlorobenzothiazole is 4.17 mg/L (measured) for Daphnia magna in the 48 h acute toxicity test according to Directive 92/69/EEC, C.2.