Soybean oil, reaction products with ethaneperoxoic acid

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 February - 13 April 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP/guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EPA-821-R-02-013

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Soybean oil, reaction products with ethaneperoxoic acid

Cas Number:

693217-63-9

Molecular formula:

C59 H106 O11

IUPAC Name:

Soybean oil, reaction products with ethaneperoxoic acid

Test material form:

liquid: viscous

Details on test material:

Agrol Polyol (CAS# 693217-63-9)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable.

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

Methanol was used as a vehicle to extract the water soluble portions of the Agrol@ Polyol product. The amount of methanol used was limited to the 100-mg/L outlined in OECD Guidelines. The test material was stirred and then allowed to equilibrate for one hour before the supernatant was used. A concurrent methanol blank was conducted to verify that this solvent did not inhibit growth.

Test solutions

Vehicle:

yes

Details on test solutions:

Methanol was used as a vehicle to extract the water soluble portions of the Agrol@ Polyol product. The amount of methanol used was limited to the 100-mg/L outlined in OECD Guidelines. A concurrent methanol blank was conducted to verify that this solvent did not inhibit growth.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

The test organism used in this study was the unicellular coccoid green alga, Selenastrum capricornutum. The original culture was obtained from Aquatic Biosystems, Fort Collins, Co. which in tum was obtained from the University of Texas Culture Collection, Austin, TX (UTEX Number 1648). The culture designation for this report is 10013.SLN. S capricornutum was cultured according to the EPA Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

No data

Test temperature:

During these tests, in all test vessels the temperature remained within the limits established in the Quality Manual. Range was 25.0 - 25.6C.

pH:

the pH range was normal. There was a noted increase in pH during the study. Increasing pH in this type of bioassay is normal as the log phase growth of algae occurs. The rate of increase is greatest toward the end of the 96-hour period. Increases of more than I pH unit toward the end of the 96-hour period are normal. Control pH ranged from 6.4 (initial) to 8.7 (after 96 hrs). Initial pH for all test groups of Agrol were within 6.4-6.5. After 96 hrs pH for all test groups of Agrol were within 7.5 - 8.8

Dissolved oxygen:

No data.

Salinity:

No data.

Nominal and measured concentrations:

0, 0 (methanol control), 6.25, 12, 25, 50 and 100 mg/L extractable fraction of Agrol.

Details on test conditions:

Lighting was accomplished with cool-white fluorescent lamps and was measured at between 360 and 440-footcandles. The photoperiod was 24-hours light.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

cell number

Effect concentrations 2

Duration:

96 h

Dose descriptor:

other: IC25

Effect conc.:

60.4 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

cell number

Effect concentrations 3

Duration:

96 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

cell number

Details on results:

See Table 1.

Results with reference substance (positive control):

No data.

Reported statistics and error estimates:

1C25 = 60.357 +/- 5.319 (CI = 42.124-74489)
IC50 = >100

Any other information on results incl. tables

Table 1 96 hour Chronic with S. capricornutum

Test Concentration	Cells/mL (x 10^6)
Lab Control, 0	1.15
Methanol Control, 0	1.17
6.25 mg/L	1.20
12.5 mg/L	1.28
25 mg/L	1.07
50 mg/L	0.97
100 mg/L	0.71
NOEC	12.5 mg/L
IC25	60.4 mg/L
IC50	>100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC was 12.5 mg/L and the IC25 and IC50 were 60.4 and >100 mg/L, respectively.

Executive summary:

A 96 -hour Algal growth inhibition study was conducted with S. capricornutum. The NOEC was 12.5 mg/L and the IC₂₅and IC₅₀were 60.4 and >100 mg/L, respectively.