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EC number: 904-155-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30/07/12 - 01/08/12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was carried out in accordance with OECD 209 and GLP standards. As such, this study is considered to be Klimisch 1.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- A nominal 5000 mg/l stock solution of HB TMP was prepared in reverse osmosis water which had been heated to 70oC. A nominal 500 mg/l solution was prepared by dilution of the 5000 mg/l stock. Test solutions were prepared by the addition of a known volume of these stock solutions to a total volume of 100 ml reverse osmosis water, synthetic sewage and activated sludge. Nominal concentrations were 10, 100 and 1000 mg/l.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge was obtained from Totnes Sewage Works, Totnes, Devon, UK, on the day of exposure of test and reference substances. This facility treats predominantly domestic sewage. The activated sludge was settled and the supernatent decanted. The sludge was fed with 50 ml of OECD synthetic sewage feed per litre of sludge per day and aerated at room temperature until the test. The total filterable solids concentration was determined to be 7759 mg/l. The pH was 6.9, which was adjusted to 7.2 with 2M sodium hydroxide prior to use.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- No information provided
- Test temperature:
- 20± 2oC
- pH:
- 7.4-8.1
- Dissolved oxygen:
- no information provided
- Nominal and measured concentrations:
- Nominal concentrations: 10, 100 and 1000 mg/l
- Details on test conditions:
- See methods below
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlrophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- In test flasks dosed with HB TMP, a non-dosed response was observed and < 13% inhibition was observed in all tested concentrations. Respiration rate of the abiotic control was negligible throughout the test. Based on these results the 3-hour EC50 was determined to be > 1000mg/l, while the 3-hour NOEC was determined to be 1000 mg/l, as percentage inhibition less than or equal to 10% was within the expected variability of the test.
- Results with reference substance (positive control):
- The 3-hour EC50 was found to be 3 mg/l. This was within the expected range for this substance and confirmed the viability of the sludge organisms.
- Reported statistics and error estimates:
- The percentage inhibition was calculated as:
{1-[respiration rate of test flask/Mean respiration rate of control flasks]} x 100 - Validity criteria fulfilled:
- yes
- Conclusions:
- Less than 13% inhibition was observed in al HB TMP concentrations. The 3-hour EC50 was estimated to be >1000 mg/L, and the 3-hour NOEC was 1000 mg/L.
- Executive summary:
A 3 -hour microbial respiration inhibition test was carried out according to OECD 209 using the test substance HB TMP. A stock solution of the test substance was created and appropriate test solutions were created by addition of activated sludge, synthetic sewage media, and reverse osmosis water. Nominal concentrations were 10, 100 and 1000 mg/L. An abiotic control was also included at 1000 mg/l. Test temperature was 20± 2oC and pH ranged between 7.4 and 8.1. The respiration rate in each flask was determined after 3 hours exposure to the test substance.
Less than 13% inhibition was observed in al HB TMP concentrations. The 3-hour EC50 was estimated to be >1000 mg/L, and the 3-hour NOEC was 1000 mg/L.
Reference
Table 1: Experimental design
Test substance | Nominal test concentration (mg/l) |
Volume of activated sludge (mL) | Volume of synthetic sewage feed (mL) | Volume reference substance stock (mL) | Volume of test substance stock (mL) |
Control | Control | 19 | 3.2 | - | - |
3,5 -DCP | 32 (abiotic) | - | 3.2 | 64 | - |
3,5 -DCP | 32 | 19 | 3.2 | 64 | - |
3,5 -DCP | 10 | 19 | 3.2 | 20 | - |
3,5 -DCP | 3.2 | 19 | 3.2 | 6.4 | - |
3,5 -DCP | 1.0 | 19 | 3.2 | 2.0 | - |
Control | Control | 19 | 3.2 | - | - |
HB TMP | 1000 (abiotic) | - | 3.2 | - | 20 |
HB TMP | 1000 | 19 | 3.2 | - | 20 |
HB TMP | 1000 | 19 | 3.2 | - | 20 |
HB TMP | 100 | 19 | 3.2 | - | 20 |
HB TMP | 10 | 19 | 3.2 | - | 20 |
Table 2: Results for pH, respiration rate and inhibition.
Test substance | Nominal test concentration (mg/L) | pH | Respiration rate (mg/L/h) | Percentage inhibition | |
Start | End | ||||
Control | Control | 7.5 | 8.1 | 31.6 | - |
3,5 -DCP | 32 (abiotic) | 7.5 | 7.4 | - | - |
3,5 -DCP | 32 | 7.5 | 8.3 | 4.8 | 86 |
3,5 -DCP | 10 | 7.4 | 8.4 | 8.9 | 74 |
3,5 -DCP | 3.2 | 7.7 | 8.4 | 16.6 | 51 |
3,5 -DCP | 1.0 | 7.6 | 8.3 | 15.2 | 55 |
Control | Control | 7.6 | 8.1 | 36.1 | - |
HB TMP | 1000 (abiotic) | 7.4 | 7.8 | - | - |
HB TMP | 1000 | 7.6 | 8.1 | 29.9 | 12 |
HB TMP | 1000 | 7.5 | 8.1 | 35.8 | <10 |
HB TMP | 100 | 7.5 | 8.0 | 29.7 | 12 |
HB TMP | 10 | 7.6 | 8.0 | 33.0 | <10 |
Description of key information
The 3-hour EC50 was found to be >1000 mg/L, and the 3-hour NOEC was found to be 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
A 3 -hour microbial respiration inhibition test was carried out according to OECD 209 using the test substance. A stock solution of the test substance was created and appropriate test solutions were created by addition of activated sludge, synthetic sewage media, and reverse osmosis water. Nominal concentrations were 10, 100 and 1000 mg/L. An abiotic control was also included at 1000 mg/l. Test temperature was 20± 2oC and pH ranged between 7.4 and 8.1. The respirtaion rate in each flask was determined after 3 hours exposure to the test substance.
Less than 13% inhibition was observed in all concentrations. The 3-hour EC50 was estimated to be >1000 mg/L, and the 3-hour NOEC was 1000 mg/L.
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