Dimethyl 2-aminoterephthalate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to current OECD-Guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl 2-aminoterephthalate

EC Number:

226-364-2

EC Name:

Dimethyl 2-aminoterephthalate

Cas Number:

5372-81-6

Molecular formula:

C10H11NO4

IUPAC Name:

1,4-dimethyl 2-aminobenzene-1,4-dicarboxylate

Constituent 2

Reference substance name:

dimethyl-2-aminoterephthalate

IUPAC Name:

dimethyl-2-aminoterephthalate

Details on test material:

purity: > 99.9 % (m/m)
no stock solution was prepared. Due to the low solubility of the material the test item was directly weight into the testing tubes.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- source of inoculum: municipal treatment plant Frankfurt-Sindlingen, Germany
- Preparation of inoculum for exposure: The activated sludge was centrifuged, sediment of activated sludge was washed, centrifuged and resuspended. The sludge was 7 days pre-ventilated to get rid of all C.
- Initial cell/bimass concentration: 10 ml/l activated suldge suspension with 16 g/l of centrifuged sediment. The dry mass of the suspension was 1,9 g/l.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

63.75 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

67 mg/L

Based on:

test mat.

Initial test substance concentration 3

Initial conc.:

65 mg/L

Based on:

test mat.

Initial test substance concentration 4

Initial conc.:

63.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

< 20

Sampling time:

28 d

Details on results:

The mean value of 4 tests in parallel was below 20 %. Therefore no graphic was produced.
The depletion of the reference substance showed an O2-reduction of > 60% witin 10 days. The O2-consumption of blind controls was < 60 mg/l (O2).
There was no significant biological degradation of the test item shown. The O2-consumtopn witin th test period of 28 d was below 20 % of theoretical maxium.

BOD5 / COD results

BOD5 / COD

Parameter:

COD

Value:

8.7 other: mg O2/l

Results with reference substance:

The depletion of the reference substance showed an O2-reduction of > 60% witin 10 days. The O2-consumption of blind controls was < 60 mg/l (O2).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The degradation rate was below 20 % after 28 d. The product is not readily biodegradable.

Executive summary:

The degradation rate was below 20 % after 28 d. The product is not readily biodegradable.