Methoxycarbonyloxycyclooct-4-ene

Short-term toxicity to aquatic invertebrates

Description of key information

The short-term toxicity of the substance to aquatic invertebrates was examined in a study according to OECD TG 202 and in compliance with GLP (Huntingdon, 1986). In this study Daphnia magna (2 replicates of 10 animals per concentration) were exposed to the substance at nominal concentrations of 0 (control), 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 under static conditions. A stock solution (1:1) of test substance in 30% Tween 80 (non-ionic surfactant and emulsifier) / dimethylformamide was prepared, and the range of test solutions prepared by direct dilution of this stock. Test substance analysis of the control and the 1.0, 3.2, 10, 32 and 100 mg/L test solutions was performed at the start and at the end of the test. As measured concentrations in the 48 -hour old test medium were found to be not within ± 20% of nominal (ca. 60 - 85% recovery), geometric mean measured concentrations were calculated. Geomean measured concentrations were 0 (control), 0.84, 2.39, 7.35, 25.9, and 87.8 mg/L. Daphnia immobility was recorded after 24 and 48 hours exposure, and the cumulative immobility at 48 hours determined. The validity criteria of the test were met. Test substance induced immobilization in Daphnia magna at nominal test concentrations of ≥5.6 mg/L, and complete immobilization was observed at 100 mg/L. Based on these findings the 48-h EC50 was determined at 21 mg/L (95% CL: 15.1-29.2 mg/L), based on geometric mean measured concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

21 mg/L