3-methylpentane-1,5-diol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was well conducted and described in accordance with GLP standards and according to an OECD guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-methylpentane-1,5-diol

EC Number:

224-709-1

EC Name:

3-methylpentane-1,5-diol

Cas Number:

4457-71-0

Molecular formula:

C6H14O2

IUPAC Name:

3-methylpentane-1,5-diol

Specific details on test material used for the study:

- Substance type: Colourless, clear liquid
- Physical state: liquid
- Analytical purity: 99.8%
- Impurities (identity and concentrations): 0.04% water; less than 0.02% CH3COOH.
- Lot/batch No.:WDK5014
- Stability under test conditions: stable (IR-tested)
- Storage condition of test material: stored in a refrigerator

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- A stock test solution was prepared by adding the test substance to dilution water (c= 10,000 mg/L).
From this stock solution, 4 actual test solutions (for 4 vessels) were prepared by further diluting the solution to 1,000 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: US EPA Environmental research laboratory.
- Age at study initiation : immature
- Method of breeding: 2-4 week old, subcultured daphinas were used as parent for the immatures used in this test.
- Feeding during test:no
- Food type: unicellular algae
- Amount: 0.1 - 0.2 mg carbon/day/water flea.
- Frequency: daily during preparation phase. No feeding during test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

55.6

Test temperature:

20°C +/- 1°C

pH:

7.4 - 8.0

Dissolved oxygen:

8.4 - 8.8 mg/L

Nominal and measured concentrations:

nominal: 1,000 mg/L.

measured:
start of test: 104% of nominal value
end of test: 101% of nominal value

Details on test conditions:

TEST SYSTEM
- Test vessel: tall dish
- Type: open
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water from the city of Kurume
- Chlorine: was analyzed and observed to be lower than 0.02 mg/L

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment, but checking oh pH.
- Light intensity: Room light

TEST CONCENTRATIONS
- Test concentrations: 1,000 mg/L test solution
- Results used to determine the conditions for the definitive study: A 1,000 mg/L test solution was observed to have no harmful effects on daphnia in a preliminary study.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate; EiC50 = 0.283 mg/L after 48h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Any other information on results incl. tables

The total number of immobilized fleas in all test and control vessels was 0.

Applicant's summary and conclusion

Conclusions:

MPD was observed to cause no immobilizing effects on daphnia magnae after 48hrs at a concentration of 1,000 mg/L.

Executive summary:

Toxic effects of MPD were tested towards Daphnia magna in a 48 -hour acute immobilization test. MPD was observed to have no immobilizing effects on Daphnia magna at a concentration of 1,000 mg/L. The NOEC and EiC50 are >1,000 mg/L.