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EC number: 221-453-2 | CAS number: 3101-60-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to the O.E.C.D. test guideline 201 with GLP compliance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2210453-2
- IUPAC Name:
- 2210453-2
- Reference substance name:
- p-tert-butylphenyl-1-(2,3)-epoxypropyl ether
- IUPAC Name:
- p-tert-butylphenyl-1-(2,3)-epoxypropyl ether
- Reference substance name:
- p-tert-butylphenyl 1-(2,3-epoxy)propyl ether
- EC Number:
- 221-453-2
- EC Name:
- p-tert-butylphenyl 1-(2,3-epoxy)propyl ether
- Cas Number:
- 3101-60-8
- Molecular formula:
- C13H18O2
- IUPAC Name:
- 2-[(4-tert-butylphenoxy)methyl]oxirane
- Reference substance name:
- Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-
- IUPAC Name:
- Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-
- Test material form:
- other: Liquid at room temperature.
- Details on test material:
- As per IUCLID5 Sections 1.1. 1.2. and 4.1.
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- A stock solution of the test substance was prepared at a concentration of 20 mg/L by adding the required nominal amount of test substance in the diluent (Algal stock culture medium with EDTA up to 0.300 mg/L) , and mixing the solution for approximately 1.25 hours. Dilution of the test substance stock solution were made as necessary to achieve the required final concentrations.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The green algae, Pseudokirchneriella subcapitata was maintained in culture in Algal stock culture medium (Woods Hole) with EDTA up to 0.300 mg/L by Stillmeadow, Inc. The algal cultures were maintained with constant aeration and a temperature of 24 +/- 2°C with Continuous lighting (400 + 40 fi. c.).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- No data
- Test temperature:
- 22-25°C
- pH:
- 7.2-8.3
- Dissolved oxygen:
- Approximately 100% saturation.
- Salinity:
- Freshwater based algal culture medium.
- Nominal and measured concentrations:
- 0, 0.625, 1.25, 2.5, 5 and 10 mg/L nominal.
- Details on test conditions:
- 250-mL sterile Erlenmeyer flasks with stoppers and aeration tubing were used for test substance. These test containers received constant aeration. The test cultures were divided into 250-mL flasks containing the appropriate concentration of test substance (three flasks for each test concentration and six flasks for the untreated control). A positive control group consisted of three flasks treated with 10 mg/L zinc chloride The flasks were set on a shaker table for constant agitation at approximately 100 cycles/minute. At 24,48 and 72 hours following dosing, observations of inhibition were made for each test container by measuring the mean cell density with a hemacytometer. The temperature was recorded daily, and the pH of each container was recorded at study termination.
- Reference substance (positive control):
- yes
- Remarks:
- Zinc chloride at 10 mg/L.
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 8.3-9.3 mg/L
- Details on results:
- Generally, growth inhibition was not observed until 72 hr following initiating of treatment. After 72 hr of exposure the percent grow
th inhibition at the high test substance concentration of 10 mg/L was 40.4% relatuve to the control value. - Results with reference substance (positive control):
- At 24 hr the mean relative growth of the positive control zinc chloride cultures was 21.8%. After 72 hr of treatment the relative growth of the positive control was 0.56%.
- Reported statistics and error estimates:
- EC50 95% confidence Limits = 8.3-9.3 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Algal growth inhibition was generally not observed until 72 hr of exposure had occured. The EC50 for growth inhibition was 9.0 mg/L with 95% confidence Limits of 8.3 - 9.3 mg/L.
- Executive summary:
The test substance, p-tert-butylphenyl-1-(2,3)-epoxypropyl ether was evaluated for acute toxicity to green algae in an O.E.C.D. test guideline 201 study. Algal growth inhibition was generally not observed until 72 hr of exposure had occured. The EC50 for growth inhibition was 9.0 mg/L with 95% confidence Limits of 8.3 - 9.3 mg/L.
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