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EC number: 679-769-5 | CAS number: 2675-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Neurotoxicity
Administrative data
- Endpoint:
- neurotoxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline followed
- Limit test:
- no
- Species:
- mouse
- Strain:
- other: ddY
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Male mice, 5-6 weeks of age and 29 ± 2.2 g body weight at the beginning of the experiments, were randomly placed in plastic cages (5-7 per cage) containing wooden flakes. They were fed laboratory chow and water ad libitum.
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Doses:
- for 10 weeks: 5.5 mmol/kg
- No. of animals per sex per dose:
- 6
- Control animals:
- yes
- Details on study design:
- - Treatment of Animals: Dosed with a blunt tip metal intubation needle, twice weekly; Control animals received a comparable volume of the vehicle.
- Statistics:
- Intergroup comparison was conducted by the Student's t-test.
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 11 other: mmol/kg
- Based on:
- test mat.
- 95% CL:
- > 7.4 - < 17
- Gross pathology:
- The seminiferous tubules show injuries in their epithelia in all cases. Lesions commonly seen in the epithelia are: degeneration of cells, especially of the spermatids and spermatocytes; reduction of the spermatozoa; and the presence of multinucleate giant cells. Sertoli cells and interstitial cells, however, appeared to be unaffected in all cases. Epididymis seemed histologically normal, although their relative weights were slightly reduced in some cases.
- Conclusions:
- LD50 for the test item was 11 mmol/kg (1399 mg/kg) after dosed at 5.5 mmol/kg in olive oil for 10 weeks.
11 mmol/kg = 1399 mg/kg bw
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 981
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
Test animals
- Species:
- mouse
- Strain:
- other: ddY
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Male mice, 5-6 weeks of age and 29 ± 2.2 g body weight at the beginning of the experiments, were randomly placed in plastic cages (5-7 per cage) containing wooden flakes. They were fed laboratory chow and water ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Duration of treatment / exposure:
- 10 weeks
- Frequency of treatment:
- Twice weekly
Doses / concentrations
- Dose / conc.:
- 5.5 other: mmol/kg
- Remarks:
- in olive oil for 10 weeks
- No. of animals per sex per dose:
- 6
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- - Treatment of Animals: Dose levels were so chosen, by preliminary experiments, that they produce either the least acute general symptoms of poisoning or no such symptoms at all. To examine the effect of metabolic activation, sodium phenobarbital (PB), which was prepared from phenobarbital before use, was given intraperitoneally at 50 mg/kg for five successive days per week, from one week before, up until the last week of treatment with the test compounds.
Examinations
- Neurobehavioural examinations performed and frequency:
- For the test experiments, only those animals in which rotarod performance was able to be carried out were preliminarily selected. A modified apparatus, which consisted of a 5 cm diameter, roughly surfaced PVC rod, rotated at 3 revolutions per minute, was used. Arithmetic means of the longest performance periods, in five successive 30 s trials in each rat, were calculated for every test group. For the comparison of neurotoxic potencies among compounds, ID50, a half maximal inhibition dose of the walking performance, was estimated from a plot of time course vs. response as follows: days to half maximal inhibition x 2/7 x single oral dose (mmol/kg).
- Sacrifice and (histo)pathology:
- After treatment with the test compounds for 10 weeks, mice were killed under ether anesthesia for histology and blood examination. The testis was weighed and fixed in 10% neutral formalin, processed, and embedded in paraffin. Ten-micron sections were stained with hematoxylin and eosin. Blood was taken from the right atrium with a heparinized syringe. Measurements of red and white blood cell counts, hemoglobin concentration, and hematocrit value, and differentiation of white blood cells, were conducted by routine methods.
- Statistics:
- Intergroup comparison was conducted by the Student's t-test.
Results and discussion
Results of examinations
- Body weight and weight changes:
- no effects observed
- Description (incidence and severity):
- Body Weight together with testicular weights: No significant change was seen in any of the treated groups when compared to control.
- Haematological findings:
- no effects observed
- Neuropathological findings:
- no effects observed
- Details on results:
- - Effect of PB Treatment:
Group of mice, treated with the test item, were given PB (50 mg/kg, 5/week), which has been known to elevate the activity of drug metabolizing enzymes, from one week before, up until the last week of treatment with the test compounds. Neither weakness nor ataxia developed in the groups treated with test item within 10 weeks following treatment.
Applicant's summary and conclusion
- Conclusions:
- The test item was non-neurotoxic.
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