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EC number: 611-056-6 | CAS number: 538313-26-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Due to the required volume for dissolved copper analyses, additional flasks (two per concentration with daphnids) were included in the test protocol to perform analytical measurements at intermediate time of 24 hours.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certificate is incuded in the report
- Analytical monitoring:
- yes
- Details on sampling:
- Analyses were performed on test solutions at T0h, T24h and T48h. Two additional vessels per concentration (with daphnids) were used for analysis for the sampling time 24 hours.
- Vehicle:
- no
- Details on test solutions:
- Available information has shown that the test item is not soluble in water. A stock solution was prepared in the dilution water (nominal concentration: 500.0 mg/L), in the dark under magnetic stirring, one week before initiating the Daphnia magna acute toxicity test. After filtration (PTFE syringe filter 0.45 µm pore size), the stock solution was diluted in the dilution water to achieve the required test concentrations. Most of the copper was undissolved and was removed by filtration.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna Straus 1820 (Crustacea Cladocera) coming from a continuous and balanced breeding carried out in the test facility, aged less than 24 hours at the start of the test.Culture conditions: Medium: Elendt M4Food: TetraMin + green algaeLight: 2500 lux +/- 400 lux ; 16-hours light / 8-hours dark cycleTemperature: 20 ± 2°C
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Dilution water (reconstituted water – NF EN ISO 6341): 239.4 mg/L expressed as CaCO3
- Test temperature:
- between 19.5 and 20.4°C
- pH:
- Control: between 7.7 and 7.8Test solutions: 7.8 and 8.7
- Dissolved oxygen:
- Control: between 8.7 and 8.8 mg O2/LTest solutions: 8.5 and 8.9 mg O2/L
- Salinity:
- Not available
- Conductivity:
- Dilution water (reconstituted water – NF EN ISO 6341): 648 µS/cmStock solution (500 mg/L) after filtration to 0.45 µm: 845 µS/cm
- Nominal and measured concentrations:
- 100.0 – 149.5 - 223.5 - 334.5 – 500.0 mg/L (CuDABT initial nominal concentrations)
- Details on test conditions:
- Vessels: disposable glass tubes filled with 10 ml of test solution.Number of animals: 30 daphnids divided in six groups of five animals, for each test solution and for the control. Test concentrations: 100.0 – 149.5 - 223.5 - 334.5 – 500.0 mg/L (initial nominal concentrations)Loading: 2 mL of test solution for each daphnidFeeding: none within the test periodAeration: none within the test periodEnvironmental conditions: complete darkness; temperature between 19.5 and 20.4°CRenewal of test solution: noneTime of observation: 24 h and 48 hToxic effect: immobilization (Daphnids that are not able to swim within 15 seconds after gentle agitation of the test vessel are considered to be immobilised (even if they can still move their antennae))
- Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 334.5 - < 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: No mortality to 334.5 mg/l and 100% mortality to 500.0 mg/L.
- Remarks:
- Nominal concentration equivalent respectively to 113 mg/L to 5,5’-bi-1H-tetrazole (diammonium salt) and 8.6 µg/L to dissolved copper for 334.5 mg/L and 178 mg/L to 5,5’-bi-1H-tetrazole (diammonium salt) and 13.7 µg/L to dissolved copper for 500.0 mg/L.
- Details on results:
- Stability of samples has been demonstrated during the validation study regarding the analysis of DABT (study 16-003-156922; report DRC 16-156922-07470A).
Samples were stored to 4°C until analysis. - Results with reference substance (positive control):
- The test carried out from November 23rd to 24th 2016 gave the following results: EC50 24h: 1.07 mg/L (1.03 – 1.16 mg/L).The EC50 24 hours was calculated using a logistic regression analysis with a bootstrap estimation of the 95% intervals.The EC50 24h is in the recommended range: 0.6 – 2.1 mg/L. This result confirms the appropriate sensitivity of the strain of Daphnia magna
- Validity criteria fulfilled:
- yes
- Remarks:
- In the control vessels, the immobilization of the daphnids did not exceed 10% at the end of the test. The dissolved oxygen concentration at the end of the test was > 3 mg/L in control and in test concentrations.
- Conclusions:
- In the test conditions, EC50 48h of the test item CuDABT has been determined between 334.5 and 500.0 mg/L (initial nominal concentrations).
EC50 > 334.5 mg/L. - Executive summary:
Young daphnids, aged less than 24 hours at the start of the test, are exposed to the test item CuBABT at a range of concentrations between 100.0 and 500.0 mg/L (initial nominal concentrations) for a period of 48 hours.
Immobilization is recorded at 24 hours and 48 hours.
In the test conditions, EC50 48h of the test item CuDABT have been determinate between 334.5 and 500.0 mg/L (initial nominal concentrations).
As mortamity was 100 % at 500.0 mg/L, EC50 CuDABT 48h > 334.5 mg/L
Reference
Definitive test
Results obtained for the four vessels per test concentrations are presented in tables below:
Observation at 24h
Nominal test concentrations |
Filtered stock solution |
Number of daphnids immobilised |
% of immobilization |
||||
A |
B |
C |
D |
Total |
|||
Controls |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100.0 |
20.0 |
0 |
0 |
0 |
0 |
0 |
0 |
149.5 |
29.9 |
0 |
0 |
0 |
0 |
0 |
0 |
223.5 |
44.7 |
0 |
0 |
0 |
0 |
0 |
0 |
334.5 |
66.9 |
0 |
0 |
0 |
0 |
0 |
0 |
500.0 |
100 |
5 |
5 |
5 |
5 |
20 |
100 |
Observation at 48h
Nominal test concentrations |
Filtered stock solution |
Number of daphnids immobilised |
% of immobilization |
pH |
O2(mg/L) |
||||
A |
B |
C |
D |
Total |
|||||
Controls |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
7.7 |
8.7 |
100.0 |
20.0 |
0 |
0 |
0 |
0 |
0 |
0 |
7.8 |
8.8 |
149.5 |
29.9 |
0 |
0 |
0 |
0 |
0 |
0 |
7.8 |
8.8 |
223.5 |
44.7 |
0 |
0 |
0 |
0 |
0 |
0 |
7.8 |
8.8 |
334.5 |
66.9 |
0 |
0 |
0 |
0 |
0 |
0 |
7.9 |
8.8 |
500.0 |
100 |
5 |
5 |
5 |
5 |
20 |
100 |
8.1 |
8.9 |
Description of key information
In the test conditions, EC50 48h of the test item CuDABT has been determined between 334.5 and 500.0 mg/L (initial nominal concentrations).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 334.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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