Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 31, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
1984
Qualifier:
according to
Guideline:
EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Details on test solutions:
Because the test substance was hardly soluble in water, a suspension of 0.5 g/l was prepared using Tween-80 as additive.

Test organisms

Test organisms (species):
activated sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min

Test conditions

Test temperature:
20.3 °C
pH:
7.4 - 7.7
Nominal and measured concentrations:
- Nominal concentration: 100 mg/L in duplicate
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol: 3.2, 10, 32 mg/l

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
No significant inhibition in respiration rate was recorded between the treatment, control and additive control groups (significant: > 10 %).
Results with reference substance (positive control):
EC50 (30 minutes) = 9 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Not toxic to waste-water bacteria at 100 mg/l upon an exposure time of 30 minutes.