(E)-5[(4-chlorophenyl)methylene]-2,2-dimethylcyclopentanone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study, a solubiliser was used. The measured data are below 80 % of nominal.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

131984-21-9

Cas Number:

131984-21-9

IUPAC Name:

131984-21-9

Details on test material:

- Name of test material (as cited in study report): RPA 405217 (DMBCP)
- Analytical purity: 95.6 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by dissolving 1.28 g test substance/l in synthetic water wlth Tween 80.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution): 0. 1 g Tween 80/l synthetic water)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The test animals wre bred at the testing facility since 1991 in synthetic freshwater according to DIN 38 412/L11)
- Age at study initiation (mean and range, SD): < 24 h

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

18.9 - 20.4 °C

pH:

before insertion of Daphnia: 7.8 - 8.0
after insertion of Daphnia: 7.5 - 8.0

Dissolved oxygen:

105 - 114 %

Nominal and measured concentrations:

Nominal test concentrations:
0.50, 1.00, 2.00, 4.00, 8.00, 16.00, 32.00, 64.00 mg/l

Measured test substance concentration:
<0.023 (control), 0.144 (nominal 0.5), 0.561 (nominal 2.0), 2.530 (nominal 8.0), 40.098 (nominal 64.0)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers (150 ml)
- Fill volume: 100 ml
- Renewal rate of test solution (frequency/flow rate): after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 1000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 24 and 48 hours.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Results used to determine the conditions for the definitive study: Total immobility after 24h at 50 mg/l: 80 %

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.26 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.43 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The substance is not stable. The quick photolysis of the active agent is the reason for the decrease of the active agent content between 0 and 24 hours. Starting after 24 hours and at a concentration of 16.0 mg/l a sedimentation was observed.

Results with reference substance (positive control):

EC50(24h): 1.97± 0.15 mg/l

Any other information on results incl. tables

Results based on nominal test concentrations:

LOEC(48h): 1.00 mg/l

NOEC(48h): 0.50 mg/l

EC50(48h): 4.68 ± 0.48 mg/l