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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study, a solubiliser was used. The measured data are below 80 % of nominal.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): RPA 405217 (DMBCP)
- Analytical purity: 95.6 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by dissolving 1.28 g test substance/l in synthetic water wlth Tween 80.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution): 0. 1 g Tween 80/l synthetic water)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: The test animals wre bred at the testing facility since 1991 in synthetic freshwater according to DIN 38 412/L11)
- Age at study initiation (mean and range, SD): < 24 h

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
18.9 - 20.4 °C
pH:
before insertion of Daphnia: 7.8 - 8.0
after insertion of Daphnia: 7.5 - 8.0
Dissolved oxygen:
105 - 114 %
Nominal and measured concentrations:
Nominal test concentrations:
0.50, 1.00, 2.00, 4.00, 8.00, 16.00, 32.00, 64.00 mg/l

Measured test substance concentration:
<0.023 (control), 0.144 (nominal 0.5), 0.561 (nominal 2.0), 2.530 (nominal 8.0), 40.098 (nominal 64.0)


Details on test conditions:
TEST SYSTEM
- Test vessel: glass beakers (150 ml)
- Fill volume: 100 ml
- Renewal rate of test solution (frequency/flow rate): after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 1000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 24 and 48 hours.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Results used to determine the conditions for the definitive study: Total immobility after 24h at 50 mg/l: 80 %
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.26 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.43 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The substance is not stable. The quick photolysis of the active agent is the reason for the decrease of the active agent content between 0 and 24 hours. Starting after 24 hours and at a concentration of 16.0 mg/l a sedimentation was observed.
Results with reference substance (positive control):
EC50(24h): 1.97± 0.15 mg/l

Any other information on results incl. tables

Results based on nominal test concentrations:

LOEC(48h): 1.00 mg/l

NOEC(48h): 0.50 mg/l

EC50(48h): 4.68 ± 0.48 mg/l

Applicant's summary and conclusion