Registration Dossier

Administrative data

Description of key information

Skin irritation:
according OECD 404, in compliance with GLP, RL2 (Bouffechoux, 1998): not irritating
Eye irritation:
according OECD 405, in compliance with GLP, RL2 (Bouffechoux, 1995): not irritating

Key value for chemical safety assessment

Skin irritation / corrosion

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Eye irritation

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Justification for grouping of substances and read-across

There are no data available on the skin and eye irritating properties of Multi constituent ester of pentaerythritol 2-ethylhexanoate. In order to fulfil the standard information requirements set out in Annex VII-IX, in accordance with Annex XI, 1.5, of Regulation (EC) No 1907/2006, read-across from structurally related substances was conducted.

In accordance with Article 13 (1) of Regulation (EC) No 1907/2006, "information on intrinsic properties of substances may be generated by means other than tests, provided that the conditions set out in Annex XI are met.” In particular for human toxicity, information shall be generated whenever possible by means other than vertebrate animal tests, which includes the use of information from structurally related substances (grouping or read-across).

Having regard to the general rules for grouping of substances and read-across approach laid down in Annex XI, 1.5, of Regulation (EC) No 1907/2006, whereby physicochemical, toxicological and ecotoxicological properties may be predicted from data for reference substance(s) by interpolation to other substances on the basis of structural similarity,Hexanoic acid, 2-ethyl-, 2,2-bis [ [(2-ethyl-1-oxohexyl)oxy] methyl] -1,3-propanediyl ester(CAS 7299-99-2) is selected as source substance for assessment of in vivo skin and eye irritation

Irritation/Corrosion

CAS

-- (a)

7299-99-2 (b)

Chemical name

Multi constituent ester of pentaerythritol 2-ethylhexanoate

Hexanoic acid, 2-ethyl-, 2,2-bis [ [(2-ethyl-1-oxohexyl)oxy] methyl] -1,3-propanediyl ester

MW

388.5 - 640.9g/mol

640.9 g/mol

Skin irritation

RA: CAS 7299-99-2

Experimental result: not irritating

Eye irritation

RA: CAS 7299-99-2

Experimental result: not irritating

(a) The substance subject to the REACh Phase-in registration deadline of 31 May 2013 is indicated in bold font.

(b) Reference (read-across) substances are indicated in normal font.

The above mentioned substance is considered to be similar on the basis of the similarities in structure, properties and/or activities. The available endpoint information is used to predict the same endpoint for Multi constituent ester of pentaerythritol 2-ethylhexanoate.

A detailed analogue approach justification is provided in the technical dossier (see IUCLID Section 13).

Discussion

Skin irritation

A skin irritation study according to OECD guideline 404 and in compliance with GLP is available for the structural analogue Hexanoic acid, 2-ethyl-, 2,2-bis [ [(2-ethyl-1-oxohexyl)oxy] methyl] -1,3-propanediyl ester (Bouffechoux, 1998). Three male New Zealand White rabbits received a topical application of 0.5 mL of the test substance on previously shaved and abraded skin sites under semi-occlusive conditions. The treated sites were washed 4 h after application and were evaluated 4, 24, 48 and 72 h and 4 and 7 days later. No erythema was noted in any of the animals at any reading time points resulting in the mean score of 0 for all animals. Only slight edema was visible in one animal, but this effect was fully reversible within 48 h. Consequently the mean score for edema was 0.1 in this animal, while the score for the other two animals were 0. Based on the study results, the test substance was considered to be not skin irritating.

Eye irritation

The structural analogue Hexanoic acid, 2-ethyl-, 2,2-bis [ [(2-ethyl-1-oxohexyl)oxy] methyl] -1,3-propanediyl ester was evaluated in a study with three New Zealand White rabbits in accordance with OECD guideline 405 (Bouffechoux, 1996). The readings of the treated eyes 4, 24, 48 and 72 h and 7 days post-instillation revealed slight conjunctival effects in all 3 animals animals (score 2 at 4 h; score 1 at 24 h), which were fully reversible within 48 h. As a consequence, the mean scores over 24, 48, and 72 h and over all 3 animals for redness were 0.3. The mean scores for cornea and iris were 0 in all animals. Based on the study results, the test substance was considered to be not eye irritating.

Conclusion

The available data from the surrogate substance Hexanoic acid, 2-ethyl-, 2,2-bis [ [(2-ethyl-1-oxohexyl)oxy] methyl] -1,3-propanediyl ester for skin and eye irritation indicates that Multi constituent ester of pentaerythritol 2-ethylhexanoate has no skin and eye irritating potential.

 


Justification for selection of skin irritation / corrosion endpoint:
Hazard assessment is conducted by means of read-across from a structural analogue. The selected study is the most adequate and reliable study based on the identified similarities in structure and intrinsic properties between source and target substance and overall quality assessment (refer to the endpoint discussion for further details).

Justification for selection of eye irritation endpoint:
Hazard assessment is conducted by means of read-across from a structural analogue. The selected study is the most adequate and reliable study based on the identified similarities in structure and intrinsic properties between source and target substance and overall quality assessment (refer to the endpoint discussion for further details).

Justification for classification or non-classification

The available data on skin and eye irritation of the substance do not meet the criteria for classification according to Regulation (EC) 1272/2008 or Directive 67/548/EEC, and are therefore conclusive but not sufficient for classification.