Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Rapporteur for the EU risk assessment of (3-chloro-2-hydroxypropyl)trimethylammonium chloride is the National Product Control Agency for Welfare and Health, Finland. The report is the result of in-depth work performed by experts in one Member State, working with counterparts in other Member States, Industry and public interest groups. The information reported is considered reliable.

Data source

Reference
Reference Type:
other: Published secondary source
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
not specified
Total exposure duration:
21 d

Test conditions

Nominal and measured concentrations:
0.17, 0.51, 1.52, 4.56 and 13.7 mg/L
Details on test conditions:
Renewal of the test solutions was performed 3 times a week.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.51 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
1.52 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
reproduction

Any other information on results incl. tables

A decrease in the concentrations of (3 -chloro-2 -hydroxypropyl)trimethylammonium chloride were observed within 48 hours. The amount of 2,3 -epoxypropyltrimethylammonium chloride was found to increase.The recovery rate based on the sum of the test substance (3-chloro-2-hydroxypropyl)trimethylammonium chloride and the conversion product (calculated as (3-chloro-2-hydroxypropyl)trimethylammonium chloride) was >=80%.

Applicant's summary and conclusion

Conclusions:
The long-term toxicity of (3-chloro-2-hydroxypropyl)trimethylammonium chloride to Daphnia magna was determined according to OECD guideline 211 in a semi-static study. The 21 day NOEC for reproduction rate is 0.51 mg/L. The LOEC for reproduction rate is 1.52 mg/L and EC50 is between 1.52 and 4.56 mg/L. The EC50 based on immobilisation was lower (1.03 mg/L) than the EC50 based on reproduction.
Executive summary:

The long-term toxicity of (3-chloro-2-hydroxypropyl)trimethylammonium chloride to Daphnia magna was determined according to OECD guideline 211 in a semi-static study. The 21 day NOEC for reproduction rate is 0.51 mg/L. The LOEC for reproduction rate is 1.52 mg/L and EC50 is between 1.52 and 4.56 mg/L. The EC50 based on immobilisation was lower (1.03 mg/L) than the EC50 based on reproduction.