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EC number: 700-172-3 | CAS number: 634-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- ethyl 2-cyano-3-oxobutanoate
- EC Number:
- 700-172-3
- Cas Number:
- 634-55-9
- Molecular formula:
- C7 H9 NO3
- IUPAC Name:
- ethyl 2-cyano-3-oxobutanoate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Doses:
- The animals of the treated group received an effective dose of 2000 or 300 mg/kg body of the test item ETHYL-2-CYANO-3-OXOBUTANOATE :
In a first step of the study, the preparation was administered by gavge, assupplied, under a volume of 1.84 mL/kg body weight (corresponding to 2 g/kg, according to the calculated density) using a suitable syringe graduated fitted with an oesophageal metal canula.
In a second and third step of the study, 0.28 mL of the test item (corresponding to 0.3 g, according to the calculated density) was added to 1.56 mL of olive oil and manually stirred to obtain a yellow solution. The preoparation was administered by gavage under a volume of 1.84 mL/ kg body weight using a suitable syringe graduated fitted with an oesophageal metal canula. - No. of animals per sex per dose:
- Groupe treated (2000 mg/kg) : 3 female rats Rf4285 to Rf4287 (step 1)
Gropu treated (300 mg/kg) : 3 female rats Rf 4288 to 4290 (Step 2) + 3 female rats Rf 4294 to 4296 (Step 3) - Control animals:
- yes
Results and discussion
Effect levelsopen allclose all
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 300 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 500 mg/kg bw
- Mortality:
- It was noted the death of 3 rats treated at 2000 mg/kg b.w. (3/3) between 3 hours 50 minutes and
4 hours post-dose. The mortalities were preceded by an absence or a decrease in spontaneous activity
(3/3), in Preyer’s reflex (2/3), in muscle tone (2/3) and in righting reflex (1/3), a dyspnea or a
bradypnea (2/3), a partial ptosis (2/3) and a mydriasis or a myosis (2/3).
The macroscopical examination of the dead animals revealed a white coloration of the forestomach, a
greenish coloration of the corpus associated with red spots (3/3), and a bright red coloration of the
lungs (1/3).
No mortality occurred in the animals treated at 300 mg/kg b.w.
No clinical signs related to the administration of the test item were observed.
A decrease in the body weight gain was noted on day 2: +4% compared to day 0 versus +15% in the
historical control group. The animals recovered a normal body weight on day 7.
The macroscopical examination of the animals at the end of the study did not reveal treatment-related
changes.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- the LD50 of the test item is highter than 300 mg/kg body weight and lower than 2000 mg/kg body weight by oral route in the rat.
In accordance with the OCDE guideline N° 423, the LD50 cutt-off of the test item may be considered as 500 mg/kg body weight by oral route in the rat.
According to the criteria for classificatin, packing and labelling of dangerous substances and preparations in accodance with the EEC directives 67/548, 2001/59 and 99/45, the test item must be classified R22 " Harmful is swallowed". The item must be characterised by the symbol "Xn" and the warning label "Harmful".
In accordance with the GHS (regulation EC No 1272/2008, the test iem must be classified in categori 4. The signal word "Warning" and hazard statement H302 "Harmful if swallowed" are required.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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