Acetamide, N,N'-(ethenylmethylsilylene)bis[N-ethyl-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 May - 10 August, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-[ethenyl(N-ethylacetamido)methylsilyl]-N-ethylacetamide

EC Number:

643-078-7

Cas Number:

87855-59-2

Molecular formula:

C11 H22 N2 O2 Si Si(CH3)(CH=CH2)[N(CH2CH3)(C(=O)CH3)]2

IUPAC Name:

N-[ethenyl(N-ethylacetamido)methylsilyl]-N-ethylacetamide

Test material form:

solid: compact

Details on test material:

Test item
Product name:ZMAT Number 4094 103 (supplied as Dow Coming:!> 1-6008 Silane)
Physical Fonn: Liq uid
Color: Amber to brown
Odor: Amine- like odor
Lot number: 091217
Storage: keep container closed in a room temperature (15-25°C)
All the information above is supplied by Dow Corning Corp.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

10 mg/L

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

62.66

St. dev.:

2.05

Sampling time:

2 d

Any other information on results incl. tables

During the 24-hour incubation period designed to purge CO2 from test vessels, the temperature ranged from 21.6 to 22.2 qc. The temperature in the environmental chamber was recorded daily during the study and ranged from 21 ,6 to 22.3 Qc. The pH measurements are presented in Table 3. The pH of the test medium was measured to be 7.39 on day -1 and ranged from 7.23 to 7,35 on day 28.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

Based on the rate of C02 evolution during this study, methylvinylbis(n-ethylacetamido)silane can not be classified as "readily biodegradable" by the criteria above since it did not achieve 60% biodegradation within a 10-day window of reaching 10% biodegradation.
However, methylvinylbis(n-ethylacetamido )silane was extensively mineralized to C02 (i.e., 62.66% by day 28) and therefore can be classified as
'ultimately biodegradable'.

Executive summary:

The purpose of the study was to determine the potential for ultimate biodegradation of methylvinylbis(n-ethylacetamido)silane in water. The test was conducted from May 7 to June 5, 2010. The mean cumulative net percent CO2 evolved (percent biodegradation) fro the aqueous medium fortified with methylvinylbis(n-ethylacetamido)silane at 10 mg C/L was 62.66% on Day 28. The toxicity control on Day 14 was 70.33% which indicates that the test substance was not toxic to the inoculum in the test medium. The cumulative net percent CO2 evolved from the sodium benzoate procedural control was 82.78% of theoretical by day 10, thus exceedin the "pass" criteria of the test (reaching 60% or greater CO2 evolution within a 10-day window of reaching 10% biodegradation). This rapid biodegradation of sodium benzoate confirmed the presence of an active microbial population and system integrity. Based on the rate of CO2 evolution during the study, methylvinylbis(n-ethylacetamido)silane can not be classified as "readily biodegradable" by the friteria above since it did not achieve 60% biodegradation within a 10-day sindow of reaching 10% biodegradation. However, methylvinylbis(n-ethylacetamido)silane was extensively mineralized to CO2 (i.e., 62.66% by Day 28) and therefore can be classified as "ultimately biodegradable".